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Recombinant-protein production in plants is a more effective system than the mammalian cell system because it can be more easily scaled up, has a low production cost, and is animal pathogen free. In this study, we established a brazzein production system using an air-lift bioreactor and transgenic carrot cells. The system included the introduction of the brazzein gene by Agrobacterium-mediated transformation. First, transgenic carrot callus (TC12) was cultured in a liquid medium. The brazzein content was analyzed during entire culture period. Next, the bioreactor type, volume, and culture period were compared to establish a bioreactor culture system. In addition, abscisic acid (ABA) was treated as an elicitor to increase brazzein bio- synthesis. During the suspension culture, cell proliferation increased rapidly beginning at day 12. At that time, total soluble protein (TSP) and brazzein were 9.0 mg·g FW−1 and 1.2 mg·g FW−1, respectively. Brazzein content was 0.5 µg·g FW−1 (TSP 5.3 mg·g FW−1) in the balloon bioreactor. Cone and column bioreactors produced 1.1 µg·g FW−1 (TSP 5.7 mg·g FW−1) and 1.2 µg·g FW−1 (TSP 7.8 mg·g FW−1), respectively. The cone bioreactor was the most suitable for brazzein cell proliferation (6.1 g brazzein per bioreactor). After 8 h of 50 μM ABA treatment, the brazzein content had increased by 3.3-fold compared to that in the non-treated bioreactor. Overall, the results show that brazzein can be produced from carrot cell culture and an air-lift bioreactor system can be used for industrial mass production.

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기사명 저자명 페이지 원문 목차
Brazzein protein production in transgenic carrot cells using air-lift bioreactor culture Jong-Eun Han, Hyoshin Lee, Thanh-Tam Ho, So-Young Park p. 161-171
(The) effect of gamma-irradiation on the changes of photosynthetic efficiency in Kanpei (Citrus unshiu × C. sinensis) Seong Heo, Jung Min Choi, Anh Tuan Le, Chang-Ho Eun, In-Jung Kim, Yong Suk Chung p. 251-255
Establishment of bioreactor cultures for the production of chlorogenic acid and ferulic acid from adventitious roots by optimization of culture conditions in Angelica acutiloba (Siebold & Zucc.) Kitag Ho-Dong Hwang, Jong-Eun Han, Hosakatte Niranjana Murthy, Hyuk Joon Kwon, Gun-Myung Lee, Jae-Hyuk Shin, So-Young Park p. 173-182
Co-ordinated responses to endogenous and environmental triggers allow a well-timed floral transition in plants Chetan Kaur, Yong Pyo Lim, Geung-Joo Lee p. 145-159
Epigenetic repression and resetting of a floral repressor, FLC, in the life cycle of winter-annual Arabidopsis Dong-Hwan Kim p. 133-143
Image analysis as a potential tool for marker-assisted selection Yun Seok Kim, Yong Suk Chung, Seong Heo p. 243-249
Simple methods for selection of T-DNA-free segregants from offspring of gene-edited Solanum nigrum Min Ji Kim, Hong Kwan Beak, Ji Eun Choi, Eun Song Lee, Keunhwa Kim, Chul Min Kim, Soon Ju Park p. 257-264
Differential expression of ginsenoside biosynthesis-related genes at an early developmental stage of Panax vietnamensis Ly H. Luu, Hang L.B. Pham, Hue T.T. Huynh, Linh N. Nguyen, Trinh T. Vu, Hien T.T. Le p. 215-228
(The) genetic engineering of glycine betaine synthesis in tobacco improves the resistance of the photosynthetic apparatus to drought stress Gui-Ping Wang, Xiao-Min Xue, Xing-hong Yang, Ru Chen, Xue-Ping Han p. 229-242
Abscisic acid synthesis and root water uptake contribute to exogenous methyl jasmonate-induced improved tomato drought resistance Jiakun Yan, Haipeng Li, Yu Li, Nan Zhang, Suiqi Zhang p. 183-193
Peroxidase gene markers revealed genetic diversity and population structure in okra germplasm Mehtap Yildiz, Mehmet Alp Furan, Metin Koçak, Gökhan Sadık, Cansu Kuzğun, Mahdi Al-Khafaji, Noor Maiwan Bahjat p. 195-204
Insights into the evolutionary origin and expansion of the BBX gene family Liyang Yu, Zhongrui Lyu, Hong Liu, Guoyun Zhang, Caiyun He, Jianguo Zhang p. 205-214

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번호 참고문헌 국회도서관 소장유무
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