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Soybean (Glycine max (L.) Merr.) varieties with a green seed coat and cotyledon have long been cultivated for their high levels of lutein, which is considered beneficial for eye health. However, mature soybean seeds also contain major antinutritional and allergenic factors, including P34, Kunitz trypsin inhibitor (KTI), lectin, and lipoxygenase proteins. The objective of this research was to develop a new soybean line with a green cotyledon and a green seed coat, and without P34, KTI, lectin, and lipoxygenase proteins. A breeding population was developed using two cultivars and three germplasms. Seventy-four F₂ seeds with a green cotyledon and a green seed coat were obtained. Twenty-four F2 seeds with a tetra null genotype (p34p34-titi-lele-lox1 lox1lox2lox2lox3lox3) were selected and planted in a greenhouse. One F₂ plant that exhibited suitable agronomic traits was selected. The SDS-PAGE and western blot methods were used to determine the absence (tetra null genotype) of P34, KTI, lectin, and lipoxygenase proteins in random F₃ seeds from the F₂ plant selected. The color of the cotyledon and seed coat for the new selection line was green. The stem height was 72 cm and the 100-seed weight was 21.5 g. The new line obtained in this research could be bred into a soybean variety with a green cotyledon, a green seed coat, and fewer allergenic and antinutritional properties.

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기사명 저자명 페이지 원문 목차
Genome-wide identification of oleosin family and expression analysis in response to abiotic stresses in balloon flower (Platycodon grandiflorus) Eunhui Kim, Tae Kyung Hyun p. 71-76

Industrial interests and requirements for improvement of pearl millet, a gluten-free and climate-smart underutilized cereal Leila Riahi, Mériam Ben-Romdhane, Ahmed S. Masmoudi p. 77-88

Evaluating the insecticidal potential of ethanol extracts from Melia azedarach Linn. against Bactrocera cucurbitae : a pest inflicting damage on Momordica charantia Linn. Tran Thi Phuong Nhung, Le Pham Tan Quoc p. 89-99

In Silico identification of dengue NS5 phytochemical inhibitors as potential antiviral drug compounds via molecular docking Earl Adriane Cano, Jovito San Luis Ⅲ, Julia Cassandra Perez, Aleezah Priela, Eiby Grace Ramos, Mhizzy Reyes, Luis Antonio Rico, LJ Sabado p. 100-110

Genetic elements controlling starch biosynthesis and secondary growth in root and tuber crops Haruna Anate Abdulsalami, Yookyung Lim, Hyunwoo Cho p. 111-120

Solanum pinnatisectum 엽록체 전장유전체 구명 및 특이적 분자마커의 개발 = Completion of chloroplast genome sequence and development of PCR-based markers for Solanum pinnatisectum 손서연, 박태호 p. 121-128

식물에서 인의 역할과 인산염 고갈 위기 대응 방안 = Phosphate depletion : research status and challenges in agriculture 신나현, 조래현 p. 129-142

Solanum jamesii의 엽록체 전장 유전체 분석 및 이를 활용한 S. jamesii 특이적 PCR기반의 분자마커 개발 = Complete chloroplast genome of Solanum jamesii and development of PCR-based markers to discriminate between S. jamesii and other Solanum species 조주련, 박태호 p. 143-151

Development of a green soybean line with a green cotyledon and a tetra null genotype for P34, lectin, Kunitz trypsin inhibitor, and lipoxygenase proteins Sarath Ly, Kwon Moon Jeong, Byeong Eon Park, Jong Il Chung p. 152-157

Solanum verrucosum과 가지속 식물의 엽록체 전장 유전체 비교를 통한 PCR기반의 S. verrucosum 특이적 분자마커의 개발 = Development of PCR-based markers for Solanum verrucosum via comparison of the complete chloroplast genome sequence of S. verrucosum with those of other Solanum species 조주련, 박태호 p. 158-166

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