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22대 대한민국 국회 국회정보길라잡이 국회의원검색
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For stable silkworm breeding and high-quality sericulture product production, the detection of Pebrine disease in silkworm eggs is critical. Current diagnostic methods can be timeconsuming and complex. This study aimed to develop a simplified and rapid diagnostic method using loop-mediated isothermal amplification (LAMP) technology to detect pebrine infection in silkworm mother moths. Eight primer candidates targeting the ribosomal gene region of microsporidia were designed and evaluated for specificity and detection sensitivity.

A simplified nucleic acid extraction method was established, and isothermal amplification was performed using the selected primers. Of these, primers ID30 and ID45 showed no polymerization, while ID5, ID18, and ID76 exhibited nonspecific reactions, making them unsuitable. Primers ID1, ID6, ID45, and ID82 successfully amplified DNA only in the presence of pebrine, with ID82 demonstrating the best reproducibility and sensitivity, detecting as low as 2.5 pg/ul of DNA through electrophoresis and 5 pg/ul via a colorimetric change with phenol red. The entire process, from nucleic acid extraction to detection, was completed within 60 min. The use of the ID82 primer set in LAMP technology offers a promising and efficient approach for the rapid diagnosis of pebrine disease, potentially enhancing quality control in sericulture.

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기사명 저자명 페이지 원문 목차
Insect-originated functional food : nutritional benefits and applications Yoon-Jo Lee, HaeYong Kweon, You-Young Jo, Seong-Gon Kim p. [1-8, 실은 99-106]
Comparison of chemical and physical extraction methods of steamed-mature silkworm (Hongjam) protein Ji Hae Lee, Jong Woo Park, Seong-Wan Kim, Sang Kuk Kang, Seul Ki Park, Hyeok Gyu Kwon, Seong Ryul Kim p. [1-7, 실은 107-113]
Discovery of the vagrant darter, Sympetrum vulgatum (Odonata: Libellulidae) in Korea Jeong Sun Park, Jong Moon Kim, Hyeong Gi Jeon, Sung Soo Kim, Jee-Young Pyo, Iksoo Kim p. [1-10, 실은 114-123]
Field monitoring and population genetic analysis of the dragon swallowtail Sericinus montela (Lepidoptera: Papilionidae), a vulnerable species in South Korea Seung Hyun Lee, Jeong Sun Park, Jee-Young Pyo, Sung-Soo Kim, Heon Cheon Jeong, Iksoo Kim p. [1-15, 실은 124-138]
Application and evaluation of LAMP-PCR for the diagnosis of silkworm pebrine disease Jong Woo Park, Pu Reun Kook, Jeong Sun Park, Yeong Hee Cho, Seul Ki Park, Hyeok Gyu Kwon, Ji Hae Lee, Sang Kuk Kang, Seong-Wan Kim, Kee Young Kim, Seong-Ryul Kim p. [1-8, 실은 139-146]
Analyzing the nutritional value of Protaetia brevitarsis larvae feeding on coffee and oyster mushroom cultivation by-products Min Ji Park, Hyung Joo Yoon, Sun Young Kim p. [1-9, 실은 147-155]
Comparative analysis of Bombyx batryticatus and Bombyx mori on α-glucosidase inhibition and their bioactive compositions Hwa Lee, Jong-Hoon Kim p. [1-7, 실은 156-162]

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