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국회도서관 홈으로 정보검색 소장정보 검색

초록보기

M13 bacteriophage is a virus that specifically infects Escherichia coli exhibiting an F pilus. Its structure comprises five different coat proteins surrounding its genome. Since the development of phage display technology, which enables the display of foreign peptides/proteins on phage surfaces, various applications from medicine to nanomaterials have utilized phages. Notably, studies using phages as delivery tools for drugs and protective antigens have garnered significant attention. The delivery of foreign antigens via M13 phage display limits the size of the molecule expressed on the major coat protein (pVIII) to 15 amino acids. In this study, by applying the SpyTag/SpyCatcher system, we displayed a foreign antigen that cannot be displayed on the pVIII with the existing system. The conjugation reaction between SpyTag-displaying phage on the pVIII protein and SpyCatcher-fused protein was confirmed by SDS-PAGE and Western blot. In the immunization experiment in rainbow trout (Oncorhynchus mykiss), the group administered the foreign protein displaying phage showed a higher antibody titer than the group administered the foreign protein alone. These results suggest that large antigens can be displayed on the pVIII protein of M13 phage using SpyTag/SpyCatcher system. Furthermore, unlike subunit vaccines requiring adjuvants to induce a sufficient immune response, the phage can act as a self-adjuvanting agent.

권호기사

권호기사 목록 테이블로 기사명, 저자명, 페이지, 원문, 기사목차 순으로 되어있습니다.
기사명 저자명 페이지 원문 목차
Molecular surveillance of IHNV in rainbow trout farms and marine shellfish in Gangwon Province, Korea Ju Young Seo, Seyi Shin, Yu Dam Kim, Ji Yeong Lee, Seung Ry Choi, Young Chul Kim p. 159-171

Differentially expressed energy metabolism and detoxifying genes of rock bream (Oplegnathus fasciatus) during rock bream iridovirus (RBIV) infection Chamilani Nikapitiya, Sung-Ju Jung, Myung-Hwa Jung p. 173-187

Deferasirox를 활용한 돌돔이리도바이러스에 감염된 돌돔의 폐사율 개선 = Reduction in mortality rate of rock bream (Oplegnathus fasciatus) infected with rock bream iridovirus (RBIV) through the use of deferasirox 임동진, 정명화 p. 189-198

Deferoxamine mesylate salt 기반 돌돔의 혈액생화학적 분석과 돌돔이리도바이러스 감염에 의한 폐사율 개선 = Blood biochemical analysis of rock bream (Oplegnathus fasciatus) following Deferoxamine mesylate salt administration and improvement in survival rate against rock bream iridovirus (RBIV) infection 이다슬, 서한길, 정명화 p. 199-208

해수 및 저질 시료에서의 흰반점바이러스(white spot syndrome virus) 검출을 위한 다양한 전처리 방법 평가 = Evaluation of various extraction methods for white spot syndrome virus (WSSV) from seawater and sediment 김민재, 신동준, 유진솔, 김광일 p. 209-222

극동산 뱀장어(Anguilla japonica)에서 Citrobacter freundii의 분리 및 특성 연구 = Isolation and characterization of Citrobacter freundii from Japanese eel (Anguilla japonica) 이은섭, 김현우, 이승준, 송준영, 권세련 p. 223-237

Polylactide-co-glycolic acid (PLGA)에 캡슐화된 Edwardsiella tarda 포르말린 사멸백신의 이스라엘 잉어(Cyprinus carpio)에 있어서의 효능 = Efficacy of polylactide-co-glycolic acid (PLGA) microparticle encapsulating Edwardsiella tarda formalin killed cell (FKC) vaccine in Israeli carp (Cyprinus carpio) 장태원, 양은총, 김재훈, 최상훈 p. 239-247

새싹 인삼 유래 Lactobacillus rhamnosus의 postbiotics 섭이에 따른 무지개송어(Oncorhynchus mykiss)의 성장 및 항병력 효과 = Effects of postbiotics from ginseng sprout-isolated Lactobacillus rhamnosus on growth performance and disease resistance of rainbow trout (Oncorhynchus mykiss) 최희재, 조성현, 정세진, 이현학, 이기만, 박진홍, 박영민, 김민수, 김준환, 강예재 p. 249-259

Enterocytozoon hepatopenaei (Microsporidia: Enterocytozoonidae) in the frozen black tiger shrimp (Penaeus monodon) imported to Korea Han-Seul Cho, Jae-Young Lee, Jeong-Ho Kim p. 261-268

Experimental infection of Enteromyxum leei to red seabream Pagrus major Young-Ghan Cho, Sung-Pyo Hur, Sang Phil Shin p. 269-275

완도 넙치(Paralichthys olivaceus) 양식장에서 발생한 여윔증 원인체 Enteromyxum leei에 대한 사례 보고 = Case report of Enteromyxum leei, the causative agent of emaciation disease in olive flounder (Paralichthys olivaceus) farm in Wando 이영준, 전려진, 오영은, 이응준, 정준범 p. 277-283

Fusion of M13 bacteriophage pⅧ protein with antigen protein using SpyTag/SpyCatcher system to increase antibody titer in rainbow trout (Oncorhynchus mykiss) Euna Choi, Mariem Bessaid, Ki Hong Kim p. 285-292

(The) effect of different feeding on the gut microbiome of Atlantic salmon (Salmo salar) in Busan, South Korea Najib Abdellaoui, Fariay Faiz, Kyung Hoon Hahm, Min Sun Kim p. 293-302

천심련(Andrographis paniculata) 추출물이 넙치(Paralichthys olivaceus)의 장에 미치는 조직학적 영향 평가 = Evaluation of the histological effects of Andrographis paniculata extract on the intestine of olive flounder (Paralichthys olivaceus) 이주노, 임새연, 김성현, 한소리, 홍지민, 이연경, 박영진 p. 303-311

넙치, Paralichthys olivaceus를 이용한 toltrazuril의 안전성 평가 = Effects of toltrazuril on olive flounder (Paralichthys olivaceus) : acute toxicity, drug metabolism, cytokine gene expression and biochemical parameters : 급성독성, 약물 대사, 사이토카인 유전자 발현 및 생리학적 지표 분석 박상협, 도정완, 조미영, 김명석, 김아란, 김이경 p. 313-329

수인성 니코틴 노출에 의한 금붕어(Carassius auratus)의 폐사율, 혈액학적 성상 및 조직병리학적 변화에서의 영향 = Effects on the mortality rate, hematological parameters and histopathological changes in goldfish, Carassius auratus exposed to waterborne nicotine 이희종, 나정현, 박지원, 권세련, 송준영 p. 331-341

에탄올 고정액의 조직병리학적, 분자생물학적 분석 유효성 평가 = Validity assessment of ethanol for fixatives of histopathological and molecular biological analysis 김민규, 우원식, 김경호, 손하정, 손민영, 박찬일, 강경식 p. 343-367

고수온기에 폐사한 양식생물의 질병 검사 개선 방안 = Improving diagnostic procedures for mass mortality events on aquaculture farms during high temperature periods 조미영, 김아란, 김형준, 이남실, 이경미, 도정완 p. 369-377

First report of Flavobacterium plurextorum infection in rainbow trout (Oncorhynchus mykiss) in Korea Kyung Min Lee, Won Joon Son, Ki Hong Kim p. 379-383

양식산 돌돔의 저수온성 부력이상증후군 증례 = A case report of buoyancy dysfunction syndrome in cultivated rock bream (Oplegnathus fasciatus) 노을빛, 윤수영, 박도건, 최희정, 김재옥, 김보성 p. 385-390