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With the growing prevalence of memory impairment and limited treatment options, this study evaluated the cognitive-enhancing potential of an Omija (

Schisandra chinensis

(Turcz). Baill) 50% ethanol extract and soybean mixture (OSM). By integrating network pharmacology with in vivo validation, we aimed to elucidate the molecular mechanisms and therapeutic efficacy of OSM. Network pharmacology was used to identify core pathways and targets associated with the memory-enhancing effects of Omija (gomisin, schizandrin) and soybean. To validate these findings, scopolamine-induced memory-impaired rats (2 mg/kg/day for 8 weeks) through the parasympathetic nervous system (PNS) suppression were administered OSM (100, 250, or 750 mg/kg/day) or dextrin (positive control) alongside a high-fat diet. Behavioral, metabolic, and molecular evaluations were conducted. Network analysis identified key targets involved in neuroinflammation including interleukin (IL)-17 and nuclear factor Kappa B (NF-κB) signaling and neurotransmitter regulation (dopamine synapse), including acetylcholinesterase (AChE), prostaglandin-endoperoxide synthase-2 (PTGS2), glycogen-synthase kinase-3beta (GSK-3β), solute carrier family 6 member 3 (SLC6A3; dopamine transporter), and plasminogen activator (PLAU). In vivo, OSM at 250 and 750 mg/kg significantly improved memory performance across passive avoidance, Y-maze, Morris water maze, and novel object recognition tests. These improvements were associated with suppressed neuroinflammatory genes (

NF-κB, PTGS2, TNF-α, IL-17, IL-1β

), enhanced cholinergic and dopaminergic balance (reduced AChE activity, increased dopamine), improved glucose metabolism, and upregulated neurotrophic factor expression (brain-derived neurotrophic factor

,

ciliary neurotrophic factor). Furthermore, OSM enhanced hippocampal insulin signaling (increased phosphorylation of Akt and GSK-3β), improved intestinal morphology, and increased portal vein butyrate levels, suggesting modulation of the gut-brain axis. In conclusion

,

OSM enhances memory through multiple central (cholinergic, dopaminergic, neurotrophic) and peripheral (anti-inflammatory, metabolic) mechanisms via activating the gut-brain axis. These findings support OSM (250–750 mg/kg) as a promising candidate for cognitive enhancement potentially by inhibiting PNS suppression, warranting further clinical investigation.

Graphical abstract

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참고문헌 (42건) : 자료제공( 네이버학술정보 )

참고문헌 목록에 대한 테이블로 번호, 참고문헌, 국회도서관 소장유무로 구성되어 있습니다.
번호 참고문헌 국회도서관 소장유무
1 Damaging effects of a high-fat diet to the brain and cognition: A review of proposed mechanisms 미소장
2 Acute scopolamine treatment decreases dopamine metabolism in rat hippocampus and frontal cortex 미소장
3 Role of Tegmental Cholinergic Neurons in Dopaminergic Activation, Antimuscarinic Psychosis and Schizophrenia 미소장
4 Cholinergic modulation of the hippocampal region and memory function 미소장
5 Amelioration of Scopolamine‐Induced Learning and Memory Impairment by α‐Pinene in C57BL/6 Mice 미소장
6 Elevated Markers of Inflammation Are Associated With Longitudinal Changes in Brain Function in Older Adults 미소장
7 Brain-Derived Neurotrophic Factor in Brain Disorders: Focus on Neuroinflammation 미소장
8 Soybean isoflavone ameliorates cognitive impairment, neuroinflammation, and amyloid β accumulation in a rat model of Alzheimer’s disease 미소장
9 The effect of soya consumption on inflammatory biomarkers: a systematic review and meta-analysis of clinical trials 미소장
10 Scopolamine-Induced Memory Impairment in Mice: Neuroprotective Effects of Carissa edulis (Forssk.) Valh (Apocynaceae) Aqueous Extract 미소장
11 Protection against Alzheimer's disease by luteolin: Role of brain glucose regulation, anti‐inflammatory activity, and the gut microbiota‐liver‐brain axis 미소장
12 Natural products in drug discovery: advances and opportunities 미소장
13 Schisandra chinensis protects against dopaminergic neuronal oxidative stress, neuroinflammation and apoptosis via the BDNF/Nrf2/NF-κB pathway in 6-OHDA-induced Parkinson's disease mice 미소장
14 The relationship between cholinergic system brain structure and function in healthy adults and patients with mild cognitive impairment 미소장
15 Long-Term Effect of Porcine Brain Enzyme Hydrolysate Intake on Scopolamine-Induced Memory Impairment in Rats 미소장
16 Network Pharmacology Approach for Medicinal Plants: Review and Assessment 미소장
17 Danggui-Shaoyao-San Attenuates Cognitive Impairment via the Microbiota–Gut–Brain Axis With Regulation of Lipid Metabolism in Scopolamine-Induced Amnesia 미소장
18 High-fat diet exacerbates cognitive decline in mouse models of Alzheimer's disease and mixed dementia in a sex-dependent manner 미소장
19 Network Pharmacology and Bioinformatics Approach Reveals the Multi-Target Pharmacological Mechanism of Fumaria indica in the Treatment of Liver Cancer 미소장
20 Systemic inflammatory markers in relation to cognitive function and measures of brain atrophy: a Mendelian randomization study 미소장
21 Explore the Therapeutic Composition and Mechanism of Schisandra chinensis-Acorus tatarinowii Schott on Alzheimer’s Disease by Using an Integrated Approach on Chemical Profile, Network Pharmacology, and UPLC-QTOF/MS-Based Metabolomics Analysis 미소장
22 Vascular and Nonvascular Mechanisms of Cognitive Impairment and Dementia 미소장
23 The intestinal barrier in disorders of the central nervous system 미소장
24 Conventional Versus New Treatment: Comparing the Effects of Acetylcholinesterase Inhibitors and N-Methyl-D-Aspartate Receptor Antagonist With Aducanumab 미소장
25 Minocycline alleviated scopolamine-induced amnesia by regulating antioxidant and cholinergic function 미소장
26 AD−1 Small Molecule Improves Learning and Memory Function in Scopolamine-Induced Amnesic Mice Model through Regulation of CREB/BDNF and NF-κB/MAPK Signaling Pathway 미소장
27 Short chain fatty acids: the messengers from down below 미소장
28 Understanding the Gut–Brain Axis and Its Therapeutic Implications for Neurodegenerative Disorders 미소장
29 Microbiota–gut–brain axis and its therapeutic applications in neurodegenerative diseases 미소장
30 The Role of PNPLA3_rs738409 Gene Variant, Lifestyle Factors, and Bioactive Compounds in Nonalcoholic Fatty Liver Disease: A Population-Based and Molecular Approach towards Healthy Nutrition 미소장
31 2024 Alzheimer's disease facts and figures 미소장
32 Vagus Nerve Suppression in Ischemic Stroke by Carotid Artery Occlusion: Implications for Metabolic Regulation, Cognitive Function, and Gut Microbiome in a Gerbil Model 미소장
33 Moderate capsaicin-containing kochujang alleviates memory impairment through the gut-brain axis in rats with scopolamine-induced amnesia 미소장
34 The communication mechanism of the gut-brain axis and its effect on central nervous system diseases: A systematic review 미소장
35 Nanoligomers targeting NF-κB and NLRP3 reduce neuroinflammation and improve cognitive function with aging and tauopathy 미소장
36 Gut–Brain Axis and Neuroinflammation: The Role of Gut Permeability and the Kynurenine Pathway in Neurological Disorders 미소장
37 Efficacy and Mechanism of Schisandra chinensis Fructus Water Extract in Alzheimer’s Disease: Insights from Network Pharmacology and Validation in an Amyloid-β Infused Animal Model 미소장
38 An overview of the relationship between inflammation and cognitive function in humans, molecular pathways and the impact of nutraceuticals 미소장
39 Natural compounds for Alzheimer's prevention and treatment: Integrating SELFormer-based computational screening with experimental validation 미소장
40 Obesity-associated memory impairment and neuroinflammation precede widespread peripheral perturbations in aged rats 미소장
41 Clinical Practice Guidelines for Dementia: Recommendations for Cholinesterase Inhibitors and Memantine 미소장
42 Elucidating the specific mechanisms of the gut-brain axis: the short-chain fatty acids-microglia pathway 미소장