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Apple (Malus domestica) is a major fruit crop in Korea, but its production is threatened by viral infections and bacterial diseases such as fire blight. To increase production sustainability, fire blight-resistant apple rootstocks from the Cornell-Geneva (Geneva® series), particularly G11 and G30, have been studied for their disease resistance and cold tolerance. This study aimed to establish an optimized in vitro propagation protocol using apical meristem culture, as no established medium composition was available for the efficient micropropagation of fire blight-resistant apple rootstocks. Meristems (0.2-0.3 mm) were excised from the axillary buds of G11 and G30 and cultured on Murashige and Skoog (MS) medium supplemented with various plant growth regulators (PGRs; 0.5, 1.0, 1.5 mg/L BAP, 0.5 mg/L zeatin, 0.2 mg/L kinetin, 0.5, 1.0 mg/L GA₃, 0.2 mg/L 2ip, and 0.1, 0.2, 0.5 mg/L IBA) MS medium containing 2.0 mg/L BAP and 0.05 mg/L NAA showed high shoot induction rates of 95.8% (G11) and 100% (G30), with an average of 3.8 leaves per explant. The highest shoot proliferation rate (100%) and number of shoots (6.0 per explant) were obtained on MS medium supplemented with 0.5 mg/L BAP, 0.5 mg/L zeatin, 0.2 mg/L IBA, and 0.1 mg/L GA₃. Rooting rates of 100% (G11) and 75% (G30) were achieved on 1.1 mg/L MS medium containing 0.2 mg/L IBA and 2.0 mg/L IAA. Genetic stability was assessed using 15 simple sequence repeat (SSR) markers, which detected 30 alleles across 20 micropropagated plantlets. Genetic stability was confirmed by the fact that all SSR profiles were monomorphic and matched those of the donor plants. These results support the effectiveness of employing meristem culture and SSR markers for the virus-free propagation and genetic authentication of apple rootstocks.

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방풍 구조물 설치에 따른 옥수수 화분 비산 및 교차수분 저감 효과 연구 = Study of the effect of windbreaks on reducing maize pollen dispersal and cross-pollination 진병준, 한태희, 김형규, 차예진, 노동현, 서해랑, 이수진, 장예나, 김수빈, 박성열, 김민석, 정재요, 백동원, 전현진, 김민철 p. 184-196

Genetic stability analysis of in vitro micropropagated fire blight-resistant apple rootstocks Young Hee Kwon, Ga Ram Lee, Cheol Ku Yoon, So Young Park p. 197-205

Identification and functional characterization of FLOWERING LOCUS T and TWIN SISTER OF FT floral integrator genes in Pak-choi (Brassica rapa ssp. Chinensis) Hwa Hyun Jeong, Young-Cheon Kim, Hee Tae Lee, Jeong Hwan Lee p. 206-214

경정배양을 통한 딸기 품종 ‘킹스베리’와 ‘하이베리’의 조직배양묘 대량생산 = Mass production of tissue-cultured plantlets of strawberry cultivars 'kingsberry' and 'highberry' through shoot tip culture 모숙연, 오수아, 최수빈, 박한용 p. 215-222

엽록체 전장유전체 정보를 이용한 PCR 기반의 S. iopetalum 및 S. mochiquense 특이적 분자마커의 개발 = Development of PCR-based markers specific to Solanum iopetalum and S. mochiquense by using complete chloroplast genome sequences of Solanum species 조주련, 박태호 p. 223-232

Solanum kurtzianum 엽록체 전장유전체 분석 및 이를 이용한 S. kurtzianum 특이적 PCR 기반의 분자마커 개발 = Completion of chloroplast genome sequence of Solanum kurtzianum and development of PCR-based markers specific to S. kurtzianum 손서연, 박태호 p. 233-240

감자 근연야생종 Solanum vernei 엽록체 전장유전체 구명과 이를 이용한 S. vernei 특이적 분자마커의 개발 = Identification of chloroplast genome sequence of Solanum vernei and development of PCR-based markers specific to S. vernei 손서연, 박태호 p. 241-248

Stress-inducible FBS1 family regulates cell division and salt stress tolerance in Arabidopsis thaliana Kyoug Rok Geem, Wonsil Bae, Hojin Ryu p. 249-255

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