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Release of prosthesis debris at the tissue-implant interface is a major cause of aseptic loosening, a phenomenon that requires premature replacement of the prosthesis. The main objective of this paper is to propose a step-structured modus operandi for a reliable scanning electron microscopy (SEM) analysis of debris released from prostheses into the surrounding tissues. Following a proven methodology for the analysis of this wear debris would allow research and hospital laboratories to reduce time and obtain results associated with a common protocol and consequently, more comparable results. For developing the methodology, we chose the clinical case of a hip prosthetic revision, in which a Cr-Co head misalignment caused the wearing out of the polyethylene acetabular insert and a partial wear of the Ti-6Al-4 V acetabular cup. Samples of periprosthetic tissues, after being partially digested in a KOH basic solution, were investigated in vivo and in situ with SEM observations and Energy Dispersive X-rays Spectroscopy (EDXS) analyses. Although developed from a specific case study, this methodology is compatible and applicable to other standard cases as well. Regarding the set of samples we selected, a complete set of micro- and nano-structural analysis, compositional spectra and high-resolution images have been acquired, showing the morphology of the debris involved, and the agglomeration phenomena occurring in the tissue. The proposed protocol complements previous studies on tribological phenomena, underlying debris production at the tissue-prosthesis interface, best digestion techniques for fragment isolation, and nanotoxicology.

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Bispecific antibodies and CLEM : an analytical approach to advanced cell imaging for therapeutic strategies Han-ul Kim, Young Kwan Kim p. 1-7

Phase transition of amorphous cobalt hydroxide to crystalline cobalt oxides by electron-beam irradiation Minjeong Lee, Hye Seong Jang, Gayoung Yoon, Gyeong Hee Ryu p. 1-6

Specimen preparation for X-ray micro‑computed tomography of forest pests Eun Jung Ahn, Ki Woo Kim p. 1-12

Optical clearing of apple tissues for in vivo imaging of the pathogenic behavior of the fungus Botryosphaeria dothidea on host surfaces Ki Woo Kim, Eun Woo Park p. 1-6

Morphological characteristic analysis of unrecorded freshwater diatoms in Korea Daeryul Kwon, Kyeong-Eun Yoo, Hyeonjin Cho, Sukmin Yun, Chaehong Park p. 1-17

Technical approaches of 3D reconstruction from protein complex using the mixture of differently stained images : providing suggestive evidence for improving its resolution Yoon Ho Park, Myeong Seon Jeong, Gang San Song, Tak gwonbaek, Young Kwan Kim, Hyun Suk Jung p. 1-9

Advancing atomic electron tomography with neural networks Juhyeok Lee, Yongsoo Yang p. 1-12

Studies on PVA/MAA:EA polymer blend films modified by Fe3+ doping Neeruganti Obularajugari Gopal, Mahammad Hussain Basha, Ojha Pravakar, Yedurupaka Madhav Kumar p. 1-11
Junctional microstructure of the non‑sticky spiral scaffold in the golden orb web spider, Trichonephila clavata SeungMin Lee, MyungJin Moon p. 1-12
Analysis of smart imaging runtime Thomas Athey, Shashata Sawmya, Yaron Meirovitch, Richard Schalek, Pavel Potocek, Ishaan Chandok, Maurice Peemen, Jeff Lichtman, Aravinthan Samuel, Nir Shavit p. 1-8
Electron diffraction and dark-field TEM for structural analysis of 2D van der Waals materials Byunghyun Kim, Daesung Park, Siwon Jeong, Hyobin Yoo p. 1-13
Anatomy and histology of the olfactory organ of Korean amur goby Rhinogobius brunneus (Gobiiformes, Gobiidae) Hyun Tae Kim p. 1-7
Development of electron ptychography from algorithms, detectors to its applications Wonwoo Suh, Jeewon B. Choi, Keun-Yeol Park, Celesta S. Chang p. 1-11
From a clinical case to a general methodology to analyze prosthetic joint failure, by micro- and nano-characterization with SEM of intra-tissue wear debris Stefania Tarter, Lorena Maines, Giandomenico Nollo, Marco Molinari, Stefano Gialanella p. 1-15

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