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To better understand the actual status of volatile organic compound (VOC) emissions from the livestock industry in Japan, air samples were collected near the exhaust vents of a windowless commercial layer hen facility located in the northern Kanto region. Environmental parameters including temperature, relative humidity, and wind speed at the ventilation outlets were also recorded. The average VOC concentration inside the shed ranged from 151 to 448 μg m⁻3, consistent with those observed in educational hen sheds. Volatile fatty acids (VFAs) were the most abundant VOCs across all months, followed by ketones and aldehydes, together accounting for 72–91% of total VOCs. Regarding atmospheric impacts, VFAs contributed most to hydroxyl radical reactivity (OHR), while aldehydes dominated ozone formation potential (OFP). VOC emission rates (ERs) ranged from 271 to 1240 μg h⁻1 kg⁻1, with the highest value recorded in June, and were positively correlated with in-shed temperature. The estimated annual VOC emissions from commercial layer hen facilities across Japan totaled approximately 455 tons, representing ~0.1% of the country’s anthropogenic VOC emissions.

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