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With the growth of sericulture in China, the production of silkworm excrement (SE) has increased significantly.

SE can be converted into valuable biomass by housefly larvae, but its high content of cellulose, hemicellulose, and other recalcitrant substances severely limits its resource utilization. This study aims to develop an efficient system for treating SE using housefly larvae and to assess the impact of co-digestion with wheat bran (WB). The results indicate that a SE to WB ratio of 2:1 (TG2) yielded the best performance in cellulose degradation. At this ratio, the survival rate of the larvae, waste reduction, and bioconversion rate reached 92.6 %, 19.26 % (dry weight reduction), and 1.39 %, respectively. Analysis of the physicochemical properties and active substance content of the SE substrate showed increases in nutrient levels for the TG2 group (N: 16.88 %; P: 52.02 %; K: 71.29 %). Additionally, the contents of total flavonoids, alkaloids, and 1-Deoxynojirimycin (DNJ) were measured at 1.22 µg/mL, 1.99 µg/mL, and 1.64 µg/mL, respectively. The degradation rates of cellulose and hemicellulose were 85.7 % and 50.8 %, respectively. This study demonstrates that co-digestion of SE with WB enhances the efficient utilization of SE, offering new approaches for managing cellulose-rich, recalcitrant waste.

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