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목차

I. 서론 8

II. 실험재료 및 방법 13

1. 시험물질 및 실험동물 13

2. 실험동물의 사육환경 14

3. 실험방법 15

가. 시험물질 투여 및 실험동물 군구성 15

나. 실험동물의 임상관찰 16

다. 체중변화 측정 17

라. 사료 섭취량변화 측정 17

마. 흡입챔버내 환경 모니터링 17

바. 부검 18

III. 실험 결과 21

1. 시험물질 폭로농도 monitoring 21

2. 실험동물의 임상증상 23

3. 동물의 체중변화 23

4. 사료 섭취량 25

5. 뇨 검사 결과 27

6. 병리조직학적 및 혈액학적 검사 결과 29

가. 장기의 무게변화 29

나. 혈액분석 결과 38

다. 육안해부학적 및 조직학적 관찰결과 43

IV. 고찰 66

V. 결론 76

VI. 참고문헌 78

VII. Appendixes 81

표목차

[Table 1] Environmental condition in inhalation chamber during the experiment 15

[Table 2] Experimental design on the study of inhalation toxicity of Multiple organic solvents 16

[Table 3] Abbreviation, unit and analytical methods of the items 19

[Table 4] Concentration of multiple organic solvents by exposure groups 23

[Table 5] Urinary analysis in SD rats after inhaled Multiple organic solvents for 4 weeks 28

[Table 6] Effect of toluene, n-Hexane and MIBK complex inhalation on the weight of organ in the SD male Rats 30

[Table 7] Effect of toluene, n-Hexane and MIBK complex inhalation on the weight of organ in the SD female Rats 31

[Table 8] Hematological results in male SD rats after inhaled toluene, n-Hexane and MIBK for 4 weeks 39

[Table 9] Hematological results in female SD rats after inhaled toluene, n-Hexane and MIBK for 4 weeks 40

[Table 10] Biochemical serum values in male SD rats after inhaled toluene, n-Hexane and MIBK for 4 weeks 41

[Table 11] Biochemical serum values in female SD rats after inhaled toluene, n-Hexane and MIBK for 4 weeks 42

그림목차

[Photo 1] Generator of multiple organic solvents 14

[Figure 1] Changes of concentration in inhalation chamber during the experiment 22

[Figure 2] Body weight changes in male rats during of the Multiple organic solvents inhalation 24

[Figure 3] Body weight changes in female rats during of the Multiple organic solvents inhalation 25

[Figure 4] Total mean food consumption of male SD rats inhaled multiple organic solvents during the experiment 26

[Figure 5] Total mean food consumption of female SD rats inhaled multiple organic solvents during the experiment 27

[Figure 6] Effect of toluene, n-Hexane and MIBK complex inhalation on the weight of Liver 32

[Figure 7] Effect of toluene, n-Hexane and MIBK complex inhalation on the weight of Lung 33

[Figure 8] Effect of toluene, n-Hexane and MIBK complex inhalation on the weight of spleen 34

[Figure 9] Effect of toluene, n-Hexane and MIBK complex inhalation on the weight of pancreas 35

[Figure 10] Effect of toluene, n-Hexane and MIBK complex inhalation on the weight of stomach 36

[Figure 11] Effect of toluene, n-Hexane and MIBK complex inhalation on the weight of kidney 37

[Figure 12] Effect of toluene, n-Hexane and MIBK complex inhalation on the weight of Testis 38

[Figure 13] Macroscopic photographs of the liver. There were no pathological changes in the control group and experimental group. A: Control group, B: G3 43

[Figure 14] Light microscopic photographs of the liver 45

[Figure 15] High Magnification of light microscopic photographs in the portal triad of the liver 46

[Figure 16] Macroscopic photographs of the pancreas 47

[Figure 17] Light microscopic photographs of the pancreas 48

[Figure 18] Light microscopic photographs in the Langerhan's islet of the pancreas 49

[Figure 19] Macroscopic photographs of the lung 50

[Figure 20] Light microscopic photographs of the lung 51

[Figure 21] Magnification of light microscopic photographs in the lung 52

[Figure 22] Magnification of light microscopic photographs in the bronchus of the lung 53

[Figure 23] Macroscopic photographs of the kidney 54

[Figure 24] Light microscopic photographs in the cortex of the kidney 55

[Figure 25] Magnification of light microscopic photographs in the glomerulus of the kidney 56

[Figure 26] Light microscopic photographs in the medulla of the kidney 57

[Figure 27] Macroscopic photographs of the stomach 58

[Figure 28] Light microscopic photographs of the stomach 59

[Figure 29] Macroscopic photographs of the spleen 60

[Figure 30] Light microscopic photographs of the spleen 61

[Figure 31] Macroscopic photographs of the testis 62

[Figure 32] Light microscopic photographs of the seminiferous tubule in the testis 63

[Figure 33] Light microscopic photographs of the sciatic nerve 65

[Figure 34] 복합유기용제 발생장치 (I) 68

[Figure 35] 복합유기용제 발생장치 (II) 시스템 형성도 69

[Figure 36] 복합유기용제 발생장치 (II) 발생장치 구조도 70

[Figure 37] 복합유기용제 발생장치 (II) 기화기 구조도 71

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