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표제지=0,1,1

제출문=i,2,2

요약문=iii,4,4

SUMMARY=vii,8,6

CONTENTS=xiii,14,2

목차=xv,16,2

제1장 연구 개발과제의 개요=1,18,1

제1절 연구개발의 필요성=1,18,1

1. 기술적 측면=1,18,3

2. 경제ㆍ산업적인 측면=4,21,2

3. 사회ㆍ문화적 측면=6,23,1

제2절 연구개발 목표 및 내용=7,24,1

1. 총괄 연구개발 목표=7,24,2

2. 세부 및 연차별 연구개발 목표와 내용=9,26,1

제2장 국내외 기술개발 현황=10,27,2

제3장 연구개발수행 내용 및 결과=12,29,1

제1절 고성장 형질전환 돼지의 생산=12,29,1

1. 서론=12,29,2

2. 재료 및 방법=14,31,3

3. 결과 및 고찰=17,34,15

4. 결론=32,49,1

5. 참고문헌=33,50,3

제2절 세포질내 정자 직접주입법에 의한 형질전환 수정란의 생산=36,53,1

1. 서론=36,53,1

2. 재료 및 방법=37,54,2

3. 결과 및 고찰=39,56,14

4. 결론=53,70,1

5. 참고문헌=54,71,3

제3절 상동 유전자 재조합 기술에 의한 형질전환 수정란의 생산 및 검정=57,74,1

1. 서론=57,74,1

2. 재료 및 방법=58,75,4

3. 결과 및 고찰=62,79,16

4. 결론=78,95,1

5. 참고문헌=79,96,5

제4장 목표 달성도 및 관련분야에의 기여도=84,101,2

제5장 연구개발결과의 활용계획=86,103,4

영문목차

[title page etc.]=0,1,13

CONTENTS=xiii,14,4

Chapter 1. Overview of project=1,18,1

Section 1. Necessity of the project=1,18,1

1. Technical aspect=1,18,3

2. Economical & industrial aspect=4,21,2

3. Social & culture aspect=6,23,1

Section 2. Objectives of the project=7,24,1

1. Overall objectives=7,24,2

2. Subtitle and year objectlves=9,26,1

Chapter 2. Current status of the technology=10,27,2

Chapter 3. Research contents and results=12,29,1

Section 1. Production of transgenic pigs with high growth efficiency=12,29,1

1. Introduction=12,29,2

2. Materials and methods=14,31,3

3. Results and discussion=17,34,15

4. Conclusion=32,49,1

5. References=33,50,3

Section 2. Production of transgenic embryos by intracytoplasmic sperm Injection=36,53,1

1. Introduction=36,53,1

2. Materials and methods=37,54,2

3. Results and discussion=39,56,14

4. Conclusion=53,70,1

5. References=54,71,3

Section 3. Production and examination of transgenic embryos by enhanced homologous recombination technique=57,74,1

1. Introduction=57,74,1

2. Materials and methods=58,75,4

3. Results and discussion=62,79,16

4. Conclusion=78,95,1

5. References=79,96,5

Chapter 4. Achievements and contribution=84,101,2

Chapter 5. Application of results=86,103,4

칼라목차

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fig. 1 Apoptotic cell death in poreine blastocysts. Green images shows apoptosis.=22,39,1

Figure. 2 In vitro development of transgenic (GFP gene) porcine embryos and their expression.=26,43,1

fig. 1 Chromatin and microtubule organization in poreine oocyte following microinjection of sperm head. Green image shows microtubules; red shows chromatins.=40,57,1

fig. 2 Intensity of BrdU in corporation in pronucleus following injection of pig sperm. Reported values are mean±S.E.(n=5)=43,60,1

fig. 3 Laser scanning confocal microscopic images of DNA synthesis following ICSI (pig/mouse sperm); (x 630). Red image,DNA; Green image,D systhesis; M,male pronucleus; F,female pronucleus; t,tail. a) At 10 h following ICSI (pig sperm),DNA systhesis initiated male pronucleus. A) At 12 h following ICSI (pig sperm),DNA sysnthesis completely occurs in apposed two pronucleus. B) At 9 h following ICSI (mouse sperm),DNA sysnthesis initiated large female pronucleus. B) At 11 h following ICSI (mouse sperm),DNA sysnthesis completely occurs in apposed two pronucleus.=44,61,1

fig. 4 Gene expression of GFP gene. Green colour shows foreign gene expression=49,66,1

fig. 9 Restriction analysis of mutant myostatin DNA with Hpa I enzymes following PCR,M: 100 bp DNA marker,PCR: PCR product,Hpa I: PCR product with Hpa I digestion,arrows: 250 bp (upper),50 bp (lower)=70,87,1

fig. 10 PCR and Hap I digestion of porcine embryos following injection of mutant myostatin DNA-recA complex and culture for 7-9 dats. M: 100 bp marker,1-8: embryo DNA,Allows: wild allele (upper),mutant allele (middle) and Hap I fragment of mutant allele lower)=72,89,1

fig. 12 PCR analysis of pig blastocysts derived from embryos treated by intracellular sperm injction with β-actin-proniter(이미지참조)-IGF-1 DNA. Arrow is correct bands for amplification of transgene.=75,92,1

fig. 14 Southern blot analysis of piglet genomic DNA originated from ICSI with β-actin-proniter(이미지참조)-IGF-1 DNA. 20 marker means equal copy number of 20 transgenes.=77,94,1