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소장정보 검색

국회도서관 홈으로 정보검색 소장정보 검색
결과 내 검색 동의어 포함
결과 내 검색 동의어 포함

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표제지=0,1,1

제출문=1,2,1

요약문=2,3,8

SUMMARY=10,11,5

CONTENTS=15,16,2

목차=17,18,2

제1장. 연구개발과제의 개요=19,20,1

제2장. 국내외 기술개발 현황=20,21,2

제3장. 연구개발수행 내용 및 결과=22,23,1

제1절. 연구개발 수행 내용=22,23,2

제2절. 연구재료 및 방법=23,24,6

제3절. 연구개발 결과=29,30,1

1. 소의 인공수정 및 실험동물 사육=29,30,1

2. 정상소 및 임신소의 오줌 채취=29,30,3

3. 정상소,임신소의 오줌단백질 분리=32,33,7

4. 임신기간별 임신특이 단백질의 발현량 변화조사=39,40,2

5. 선발된 특이단백질의 amino acid microsequencing 분석을 통한 단백질 정성분석=41,42,4

6. 정상소,임신소의 오줌단백질의 protein fingerprint database 구축=44,45,12

7. 오줌단백질의 proteomic map 작성 및 특이단백질 선발=56,57,4

8. 선발된 특이단백질의 mass spectrometry 분석=60,61,19

9. 소임신특이 단백질에 대한 polyclonal antibody 제조 및 Western blotting[원본불량;p.84]=79,80,15

10. 소임신특이 단백질에 대한 monoclonal antibody 제조 및 Western blotting=94,95,15

11. 소의 임신진단용 Kit의 성능검정=109,110,2

제4장. 목표달성도 및 관련분야에의 기여도=111,112,1

제1절. 연구개발의 최종목표=111,112,1

제2절. 연구평가의 착안점=111,112,1

제3절. 연구개발 목표의 달성도=112,113,1

1. 제1차년도=112,113,2

2. 제2차년도=113,114,3

3. 제3차년도=115,116,3

제5장. 연구개발결과의 활용계획=118,119,2

제6장. 연구개발과정에서 수집한 해외과학기술정보=119,120,1

제7장. 참고문헌=120,121,4

영문목차

[title page etc.]=0,1,15

CONTENTS=15,16,4

Chapter 1. Overview=19,20,1

Chapter 2. Background=20,21,2

Chapter 3. Subjects and results=22,23,1

[1] Subjects=22,23,2

[2] Materials and methods=23,24,6

[3] Results=29,30,1

1. Artificial insemination and breeding of cow=29,30,1

2. Urine sampling of non-pregnant and pregnant cow=29,30,3

3. Isolation of urine proteins from non-pregnant and pregnant cow=32,33,7

4. Time-dependent comparison of cow's pregnant-specific urine proteins=39,40,2

5. Quality analysis of bovine pregnancy-associated proteins through amino acid microsequencing=41,42,4

6. Establishment of protein fingerprint database for the urine proteins of non-pregnant and pregnant cow=44,45,12

7. Proteomic Mapfor the bovine urine proteins and selection of its pregnancy-associated proteins=56,57,4

8. Mass spectrometry analysis of the bovine pregnancy-associated proteins=60,61,19

9. Production of polyclonal antibody for the bPAP 2 and Western blotting[원본불량;p.84]=79,80,15

10. Production of monoclonal antibody for the bPAP 2 and Western blotting=94,95,15

11. Efficiency evaluation of cow pregnancy tests by using diagnostic Kit=109,110,2

Chapter 4. Level of accomplishment and contribution in rerated fields=111,112,1

[1] Research goal=111,112,1

[2] Factors for research evaluation=111,112,1

[3] Level of accomplishment=112,113,1

1. 1st year=112,113,2

2. 2nd year=113,114,3

3. 3rd year=115,116,3

Chapter 5. Application plans of results=118,119,2

Chapter 6. Information from foreign researchers=119,120,1

Chapter 7. References=120,121,4

칼라목차

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Fig.4. Comparison of the urine proteins pelleted at 50% ammonuum sulfate saturation on PH 3~10 IPG gels.The non-pregnant cows was #50(A),and the cows #48(B) was pregnant for 5 months at the time of sampling.The arrow indicates the pregnancy-associated protein.=36,37,1

Fig.6. Comparison of the urine proteins pelleted at 50% ammonuum sulfate saturation on PH 4~7 IPG gels.The non-pregnant cows was #30,and the cows #46 was obtained from a kyungpook National University's dairy farm. The cows #30(A) non-pregnant and #46(B) was pregnant for 4 months at the time of sampling. The urine #K30 and #K33 was obtained from a kunwee-Gun dairy farm. The cows #K30(C) was non-pregnant and #K33(D) was pregnant for 6 months at the time of samling.=38,39,1

Fig.7. Time-dependent comparison of the pregnant cow's urine proteins pelleted at 50% ammonium sulfate saturation on PH 3~10 IPG gels. The cows No. 46 was pregnant for 1.5 (A),4(B) and 5 months(C) at the time of samling. The arrow indicates the pregnancy-associated protein.=40,41,1

Fig.10. Match set using 2-D image analysis program.The cows #K30(A) was non-pregnant and #K33(B) was pregnant for 6 months at the time of sampling.=57,58,1

Fig.12. Urine proteome map of pregnant cow.Number uses for the identification of each proteins in Table 9.=61,62,1

Table14. Result of ELISA (Enzyme-Linked lmmunosorbent Assay) test of hybridima cells after cloning process of selected clones which were previously immunized by N-terminal peptide(A) and internal peptide of bPAP 2(B),respectively,to screen a specific monoclonal antibody=101,102,1

Fig.33. Comparison of normal 2-D gel(A) and Westem blot analysis of the pregnant bovine urint sample incubated with Mono-1 monoclonal antibody as a primary antibody and anti-rabbit antibody as a second one.=108,109,1

Fig.34. Pregnancy tests by using progesterone-based diagnostic Kit made from Green Cross Life Science Co.Ltd.Korea.=110,111,1

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