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[표제지]=0,1,1

제출문=1,2,2

요약문=3,4,2

Summary=5,6,2

Contents=7,8,1

목차=8,9,1

List Of Tables=9,10,4

List Of Figures=13,14,4

제1장 서론=17,18,2

제1절 연구 개발의 필요성=19,20,5

제2절 연구 개발의 목적=23,24,4

제3절 연구 범위=26,27,5

제2장 국내외 기술개발 현황=31,32,2

제1절 국내 기술개발 현황=33,34,4

제2절 국외 기술개발 현황=37,38,4

제3장 연구개발수행 내용 및 결과=41,42,2

제1절 대상식물의 유전적 위해성 분석=43,44,1

1. 민들레속=43,44,40

2. 소리쟁이속(Rumex)=83,84,121

3. 서양등골나물(Eupatorium Rugosum)=204,205,27

4. 돼지풀속(Genus Ambrosia)=231,232,25

제2절 대상식물에 대한 생태적 위해성 평가=256,257,49

제4장 연구개발목표 달성도 및 대외 기여도=305,306,2

제1절 연구개발목표 달성도=307,308,3

제2절 대외 기여도=310,311,1

1. 대상식물의 유전적 위해성 분석=310,311,1

가. 민들레속=310,311,7

나. 소리쟁이속=316,317,5

다. 서양등골나물=320,321,5

라. 돼지풀속=324,325,7

2. 대상식물의 생태적 위해성 분석=331,332,6

3. 활용실적=337,338,4

제5장 연구개발결과의 활용계획=341,342,11

제6장 참고문헌=352,353,18

제7장 부록=370,371,2

제1절 연구 성과에 대한 공개 세미나=372,373,5

List Of Tables

Table1-1. Number Of Individuals And Populations Of Taraxacum Species Used In The Experiment=57,58,5

Table1-2. ITS SNP Molecular Markers To Distinguish Native Dandelions From Alien Ones=66,67,1

Table1-3. Trn T-L-F SNP Markers To Distinguish Native Dandelions From Alien Ones=67,68,1

Table1-4. The Degree Of Gene Flow And Reproduction Mode Of Dandelions=73,74,1

Table1-5. The Frequency And Direction Of Hybrid Formation In Facultative Agamospermous Species Of Native Dandelions=74,75,1

Table1-6. The Frequency And Direction Of Hybrid Formation In Facultative Agamospermous Species Of Native And Alien Dandelions=75,76,1

Table1-7. Alteration Of Morpho Type And Genome Type Of Native And Alien Dandelions In Gangwha Populations=77,78,1

Table1-8. Alteration Of Morpho Type And Genome Type Of Native And Alien Dandelions In Myeonggye-ri(Hongchun-gun, Gangwon Province) Population=78,79,1

Table1-9. Alteration Of Morpho Type And Genome Type Of Native And Alien Dandelions In Dong-gang(Gangwon Province) Population=79,80,1

Table2-1. Infrageneric Classification Of The Genus Rumex Proposed By Love(1944, 1956) And Rechinger(1937, 1949b, 1954b, 1990)(이미지참조)=86,87,1

Table2-2. Taxa Of The Genus Rumex In Korea Recognized By Various Authors=87,88,2

Table2-3. List Of Taxa And Populations Of The Genus Rumex Collected During The Study Period=91,92,1

Table2-4. Taxa Of The Genus Rumex Currently Distributed In South Korea=105,106,1

Table2-5. Voucher Information For ITS Sequencing Of The Genus Rumex In Korea=149,150,1

Table2-6. Voucher Information Of Rumex Taxa Used For Sequencing Of cpDNA psbA-trnH IGS And/Or rbcL-accD IGS Region=150,151,3

Table2-7. Voucher Information For PCR-RFLP Analysis Of The Genus Rumex In Korea=161,162,3

Table2-8. Lengths G+C Contents Of ITS Regions From Korean Taxa Of The Genus Rumex=166,167,1

Table2-9. Sequence Divergences Of ITS Regions Among Seven Rumex Taxa In Korea. Observed Number Of Nucleotide Differences And Pairwise Sequence Divergences Calculated By Kimura Two-Parameter Method(Kimura, 1980) Are Given Below And Above The Diagonal, Resp=169,170,2

Table2-10. Lengths And G+C Contents Of psbA-trnH IGS Region Of Nine Korean Rumex Taxa And R. Acetosella From Finland=172,173,1

Table2-11. Sequence Divergences Observed In The cpDNA psbA-trnH IGS Region Of Nine Korean Rumex Taxa. Observed Number Of Nucleotide Differences And Pairwise Sequence Divergences Calculated By Kimura Two-Parameter Method(Kimura, 1980) Are Given Below And A=174,175,1

Table2-12. Lengths And G+C Contents of rbcL-accD IGS Region Of Nine Korean Rumex Taxa And R. Acetosella From Finland=177,178,1

Table2-13. Sequence Divergences Of rbcL-accD IGS Region Among Eight Korean Rumex Taxa. Observed Number Of Nucleotide Differences And Pairwise Sequence Divergences Calculated By Kimura Two-Parameter Method(Kimura, 1980) Are Given Below And Above The Diagon=180,181,1

Table2-14. Haplotypes From The Combined Data Set Of cpDNA psbA-trnH And rbcL-accD IGS Sequences From Nine Korean Rumex Taxa And R. Acetosella From Finland=184,185,1

Table2-15. Distribution Of cpDNA rbcL-accD Haplotypes In Populations Of R. Acetosella=191,192,1

Table2-16. ISSR Primer Sequences, Size Of Fragment Scored And Their Polymorphism(%) Generated From 200 Samples Of Rumex Acetosella=197,198,1

Table2-17. ISSR Band Frequencies Observed From Populations Of R. Acetosella=198,199,1

Table3-1. Comparison Of Diagnostic Characters Between Alien And Native Species Of Eupatorium In Korea=209,210,1

Table3-2. Collection Data Of Eupatorium Rugosum In Korea=214,215,1

Table3-3. Collection Data Of Populations Of Eupatorium Rugosum In Hakone And Tokyo In Japan=215,216,1

Table3-4. Individuals Selected From Korea For Analysis Of ITS Sequence=216,217,1

Table3-5. Individuals Selected From Korea For Analysis Of ITS Sequence=217,218,1

Table3-6. Genetic Variation Among Populations Based On The Comparison Of ITS Sequence Data=221,222,1

Table3-7. Molecular Markers Of ITS Sequences=223,224,1

Table3-8. Distribution Patterns Of Various Molecular Markers In Korean Populations=225,226,1

Table3-9. Distribution Patterns Of Various Molecular Markers In Japanese Populations=225,226,1

Table4-1. List Of Species Included In Genus Ambrosia=232,233,1

Table4-2. Collection Data And ITS Types Of Ambrosia Artemisiifolia(Aa) And A. Trifida(At) For The Genetic Variation Study=238,239,3

Table4-3. Information Of The SSR Primers Developed In The Present Study=241,242,1

Table4-4. List Of SSR Primers(UBC) Employed In This Study=243,244,1

Table4-5. Size Of ITS Regions Of A. Artemisiifolia And A. Trifida=244,245,1

Table4-6. List Of SSR Primers And Number Of DNA Fragment After PCR Experiment=251,252,1

Table5-1. Ecological Characteristics Of Alien Plants For Harmful Effect Assessments=258,259,1

Table5-2. Soil Physical And Chemical Characteristics In Fixed Study Site(n=3, Unit=Mean±S.E)=271,272,1

Table5-3. HPLC Analysis Of Phenolic Compounds In Several Alien Plants(Unit=㎎/L)=292,293,1

Table5-4. Removal Efficiency Index Of Studied Species=299,300,1

Table5-5. Removal Efficiency Index Of Eupatorium Rugosum With Different Time And Methods=300,301,1

List Of Figures

Fig.1-1. Location Of Collection Site Of Taraxacum Numerals In Circle Indicates Number Of Individuals Or Populations Collected. Asterisk Indicates Populations Containing Presumable Hybrids=62,63,1

Fig.1-2. Phylogeny Of 500 Individuals Of Dandelions Based On ITS Sequences=69,70,1

Fig.1-3. Phylogeny Of 500 Individuals Of Dandelions Based On trn T-L-F Sequences=71,72,1

Fig.2-1. Rhizome Of R. Acetosella In Jeju Populations=92,93,1

Fig.2-2. Representative Lower Cauline Leaves Of The Genus Rumex In Korea. A.R. Acetosa;B. R. Acetosella;C. R. Longifolius;D. R. Patientia;E. R. Crispus;F. R. Japonicus;G. R. Obtusifolius;H. R. Nipponicus;I. R. Maritimus;J. Ulleung Island Population=95,96,1

Fig.2-3. Inflorescence And Flower Fascicles Found In The Genus Rumex A. Diagram Of An Imflorescence;B. Scanning Electron Micrograph Of A Flower Fascicle Of R. Crispus;C. Diagram Of A Flower Fascicle Of R. Crispus=99,100,1

Fig.2-4. Representative Flower Of The Genus Rumex. A. Flower Of R. Crispus;B. Floral Diagram=102,103,1

Fig.2-5. Scanning Electron Micrographs Of Achene (A) And Achene Surface (B) Of The Genus Rumex=102,103,1

Fig.2-6. Representative Valves Of The Genus Rumex In Korea. A. R. Acetosa;B. R. Acetosella;C. R. Longifolius;D. R. Patientia;E. R. Crispus;F. R. Japonicus;G. R. Obtusifolius;H. R. Nipponicus;I. R. Maritimus;J. Ulleung Island Population=103,104,1

Fig.2-7. Distribution Of R. Acetosella In Korea=107,108,1

Fig.2-8. Distribution Of R. Crispus In Korea=115,116,1

Fig.2-9. Distribution Of R. Obtusifolius In Korea=121,122,1

Fig.2-10. Distribution Of R. Nipponicus In Korea=123,124,1

Fig.2-11. Distribution Of R. Acetosa In Korea=126,127,1

Fig.2-12. Distribution Of R. Longifolius In Korea=131,132,1

Fig.2-13. Distribution Of R. Patientia In Korea=134,135,1

Fig.2-14. Distribution Of R. Japonicus In Korea=135,136,1

Fig.2-15. Distribution Of R. Maritimus In Korea=143,144,1

Fig.2-16. Organization Of The ITS Regions Of Nuclear Ribosomal DNA. Arrows Indicate Orientation And Approximate Position Of Primer Sites=156,157,1

Fig.2-17. psbA-trnH IGS Region Of Chloroplast DNA And Primers Used For PCR And Sequencing Reaction=157,158,1

Fig.2-18. rbcL-accD IGS Region Of Chloroplast DNA And Primers Used For PCR And Sequencing Reaction=157,158,1

Fig.2-19. Aligned Sequences Of ITS Regions Of Seven Taxa Of The Genus Rumex. See Tables 2-5 And 2-8 For Taxon Acronyms. Dashes(-) Indicate Gaps And Dots(.) Indicate Matched Sequences To The First Taxon=167,168,2

Fig.2-20. Aligned Sequences Of psbA-trnH IGS Region From Nine Korean Taxa Of The Genus Rumex. See Table 2-6 And 2-10 For Taxon Acronyms. Dashes(-) Indicates Gaps And Dots(.) Indicate Matched Sequences To The First Taxon=173,174,1

Fig.2-21. Aligned Sequences Of rbcL-accD IGS Region From Nine Korean Taxa Of The Genus Rumex. See Tables 2-6 And 2-12 For Taxon Acronyms. Dashes(-) Indicates Gaps And Dots(.) Indicate Matched Sequences To The First Taxon=178,179,2

Fig.2-22. Neighbor-Joining Tree From The Combined psbA-trnH And rbcL-accD IGS Sequence Data From Korean Rumex Species. See Table 2-6 For Taxon Acronyms. OFF Indicates Rhmex Officinale;ALE R. Alexandrea=185,186,1

Fig.2-23. Two Hypotheses Proposed For The Pollen Capture In R. Crispus, A. Pollen Of R. Crispus Fertilized A Unknown Taxa With Haplotype 7. B. Pollen Of R. Crispus With Haplotype 7 Fertilized R. Patientia, R. Japonicus, And R. Longifolius=186,187,1

Fig.2-24. Agarose Gel Electrophoretic Patterns Of 10 Haplotypes Founded In Korean Rumex Taxa=190,191,1

Fig.2-25. Distribution Of cpDNA rbcL-accD IGS Haplotypes Of R. Acetosella In 2% Agarose Gel Electrophoresis. Stars(*) Indicate Haplotype 4(562 bp PCR Fragment). The Rest Are Haplotype 5(552 bp PCR Fragment)=192,193,2

Fig.2-26. Locations Of 24 R. Acetosella Populations Collected From Korea. Frequency Of Haplotype 4 Is Followed By That Of Haplotype. 5 After A Slash In Each Population=194,195,1

Fig.2-27. UPGMA Phenogram Based On ISSR Analysis Of 200 Rumex Acetosella Samples. Star(*) Indicates The Location Where The Tree Was Broken. Sample Numbers, Population Names, And Chloroplast Haplotype Numbers Are Given=199,200,1

Fig.3-1. Distribution Of Eupatorium Rugosum In Korea=207,208,1

Fig.3-2. External Morphology Of Eupatorium Rugosum=210,211,1

Fig.3-3. Rhizome Type Of Eupatorium Rogosum. Short Rhizomes With Two Aerial Stems, Short Rhizomes With 3 Aerial Stems, And Long Rhizomes=212,213,1

Fig.4-1. Leaf Shape Of Ambrosia Artemisiifolia(A) And A. Trifida(B)=233,234,1

Fig.4-2. Male Head(A) And Flower(B), Female Head Of Ambrosia Artemisiifolia(Right Side)=234,235,1

Fig.4-3. Achene Of A. Artemisiifolia Enclosed By Involucere(A). 5-6 Spines Are Developed As The Schene Matures. Lenth Of Achene Is Ca. 4㎜. About 40-200 Achenes Are Produced From Single Individual=235,236,1

Fig.4-4. Development Of Achene In Ambrosia Tifida. 1-3 Female Flowers Are Enclosed By Involucre. 5-6 Spines Begin To Grow After Fertilization. Note Long Stigma In The Female Flower(Center). Achenes Grow Upto 8 ㎜ And 100-500 Achenes Are Produced From Sing=235,236,1

Fig.4-5. Result Of PCR-RFLP(AluI, HaeIII) For 10 Representative Individuals(Left->Right : Aa25, Aa35, Aa38, Aa40, Aa47, Aa54, Aa58, Aa60, Aa64, Aa69) Of Ambrosia Artemisiifolia In Korea. Monomorphic Patterns Are Evident In Two Different Restriction Enzyme=241,242,1

Fig.4-6. SSR Sequence Data Of Representative Individuals Of Ragweed. Mono-Allelic Pattern Is Evident=242,243,1

Fig.4-7. Aligned ITS Sequences From A. Artemisiifolia And A. Trifida. ITS 1 : 1-263;5.8S : 265-425;ITS 2 : 426-652=246,247,2

Fig.4-8. Types Of ITS Sequences Detected From A. Artemisiifolia(Left) And A. Trifida(Right). Refer Table 4-2 For Detailed Information On The ITS Types=248,249,1

Fig.4-9. Phylogenetic Relationship Of ITS Types Detected From A. Artemisiifolia(A-a) And A. Trifida(A-t). Type 1, 9, 11 Appeared As Most Ancient Types Among The 18 Types Included For The Analyses=249,250,1

Fig.4-10. Distribution Of A. Artemisiifolia ITS Types In Korea=252,253,1

Fig.4-11. UPGMA Tree Of A. Artemisiifolia And A. Trifida Based Of ISSR Data=254,255,1

Fig.5-1. Chemical Structure Of Toxic Tremetone=259,260,1

Fig.5-2. Pollen Picture Of Ambrosia Artemisiaefolia(×100)=261,262,1

Fig.5-3. Diurnal Pollen Deposition Of Ambrosia Artemisiaefolia(grains/㎠/hour)=263,264,1

Fig.5-4. Pollen Deposition Of Ambrosia Artemisiaefolia With Distance(grains/㎠/day)=264,265,1

Fig.5-5. Pollen Deposition Of Ambrosia Artemisiaefolia With Direction(grains/㎠/day)=265,266,1

Fig.5-6. Removal Experiment Sites Of Rumex Acetocella a) Before Removal, b) After Removal=269,270,1

Fig.5-7. Mean Numbers Of Taraxacum Officinale In Fixed Study Sites(Plot Size=50×50㎝;Con.=Control Plots, Exp.=Experimental Plots, n=10)=272,273,1

Fig.5-8. Relative Cover Of Taraxacum Officinale In Fixed Study Sites(Plot Size=50×50㎝;Con.=Control Plots, Exp.=Experimental Plots, n=10)=273,274,1

Fig.5-9. Relative Cover Of Rumex Acetocella In Fixed Study Sites(Plot size=50×50㎝;Con.=Control Plots, Exp.=Experimental Plots, n=10)=274,275,1

Fig.5-10. Numbers Of Eupatorium Rugosum In Fixed Study Sites(Plot size=1×1m;Con.=Control Plots, Exp.=Experimental Plots, n=10)=276,277,1

Fig.5-11. Relative Cover Of Eupatorium Rugosum In Fixed Study Sites(Plot Size=1×1m;Con.=Control Plots, Exp.=Experimental Plots, n=10)=276,277,1

Fig.5-12. Numbers of Ambrosia Artemisiaefolia In Fixed Study Sites(Plot Size=1×1 m;Con.=Control Plots, Exp.=Experimental Plots, n=10)=277,278,1

Fig.5-13. Relative Cover Of Ambrosia Artemisiaefolia In Fixed Study Sites(Plot Size=1×1m;Con.=Control Plots, Exp.=Experimental Plots, n=10)=278,279,1

Fig.5-14. Species Diversity Index In Control And Removal Sites. a) : Taraxacum Officinale b) : Rumex Acetocella c) : Eupatorium Rugosum d) : Ambrosia Artemisiaefolia=280,281,2

Fig.5-15. Relative Cover Presented By Life Forms. a) : Taraxacum Officinale b) : Rumex Acetocella c) : Eupatorium Rugosum d) : Ambrosia Artemisiaefolia(TO : Taraxacum Officinale, RA : Rumex Acetocella, ER : Eupatorium Rugosum, AA : Ambrosia Artemisiaefoli=283,284,2

Fig.5-16. Allelopathic Experiments With Phenolic Compounds=289,290,1

Fig.5-17. Phenolic Compount Contents In Several Alien Plants(Unit=㎎/g, Mean±S.E., n=3) (ER : Eupatorium Rugosum, OO : Oenothera Odorata, EC : Erigeron Canadensis, AA : Ambrosia Aremisiaefolia, EH : Erechtites Hieracifolia, BF : Bidens Frondosa, AP : A=290,291,1

Fig.5-18. Germination Rates Of Tested Plants With Several Phenolic Compound Treatments(Zoysia Sp., Lactuca Sativa, Raphanus Sativus)=294,295,2

Fig.5-19. Root Growth Of Tested Plants With Several Phenolic Compound Treatments=296,297,2

영문목차

[title page etc.]=0,1,5

Summary=5,6,2

Contents=7,8,2

List Of Tables=9,10,4

List Of Figures=13,14,4

I. Introduction=17,18,2

1. Necessity=19,20,5

2. Aims=23,24,4

3. Scope=26,27,5

II. Current Status Of Development=31,32,2

1. Domestic Status=33,34,4

2. Foreign Countries=37,38,4

III. Results=41,42,2

1. Analysis Of Genetic Harmful Effects=43,44,1

1) Taraxacum=43,44,40

2) Rumex=83,84,121

3) Eupatorium=204,205,27

4) Ambrosia=231,232,25

2. Analysis Of Ecological Harmful Effects=256,257,49

IV. Achievements And Contributions To The Public=305,306,2

1. Achievements=307,308,3

2. Contributions To The Public=310,311,1

1) Analysis Of Genetic Harmful Effects=310,311,1

(1) Taraxacum=310,311,7

(2) Rumex=316,317,5

(3) Eupatorium=320,321,5

(4) Ambrosia=324,325,7

2) Analysis Of Ecological Harmful Effects=331,332,6

3) Publication And Presentation=337,338,4

V. Application Plan=341,342,11

VI. Literature Cited=352,353,18

VII. Appendix=370,371,2

1. Open Seminar=372,373,5

칼라목차

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Fig. 5-15. Relative Cover Presented By Life Forms=283,284,2