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22대 대한민국 국회 국회정보길라잡이 국회의원검색
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Title page 1

Contents 1

INTRODUCTION 5

Annex : DEFINITIONS 9

APPENDIX I: In Chemico Skin Sensitisation: Direct Peptide Reactivity Assay (DPRA) 13

APPENDIX II: In Chemico Skin Sensitisation: Amino acid Derivative Reactivity Assay (ADRA) 29

APPENDIX III: In Chemico Skin Sensitisation: kinetic Direct Peptide Reactivity Assay (kDPRA) 50

Appendix Tables 19

APPENDIX I: In Chemico Skin Sensitisation: Direct Peptide Reactivity Assay (DPRA) 19

Table 1. Cysteine 1:10/lysine 1:50 prediction model 19

Table 2. Cysteine 1:10 prediction model 19

APPENDIX I, ANNEX 2 27

Table 1. Recommended proficiency substances for demonstrating technical proficiency with the Direct Peptide Reactivity Assay 27

APPENDIX II: In Chemico Skin Sensitisation: Amino acid Derivative Reactivity Assay (ADRA) 37

Table 1. NAC/NAL prediction model 37

Table 2. NAC-only prediction model 38

APPENDIX II, ANNEX 2 47

Table 1. Recommended chemicals for demonstrating technical proficiency with ADRA_4 mM 47

APPENDIX III: In Chemico Skin Sensitisation: kinetic Direct Peptide Reactivity Assay (kDPRA) 57

Table 1. kDPRA prediction model 57

APPENDIX III, ANNEX 2 63

Table 1. Recommended proficiency substances for demonstrating technical proficiency with the kinetic Direct Peptide Reactivity Assay 63

Appendix Figures 20

APPENDIX I: In Chemico Skin Sensitisation: Direct Peptide Reactivity Assay (DPRA) 20

Figure 1. Flow-chart of the DPRA prediction model (mean depletion) taking into account borderline ranges and multiple runs to conclude... 20

APPENDIX II: In Chemico Skin Sensitisation: Amino acid Derivative Reactivity Assay (ADRA) 35

Figure 1. Procedure to assess NAC/NAL depletion in ADRA including a gravimetric approach for mixtures and alternative fluorescent detection... 35

Figure 2. Flow-chart of the ADRA prediction model (mean depletion) taking into account borderline ranges and multiple runs to conclude... 38

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