본문 바로가기 주메뉴 바로가기
국회도서관 홈으로 정보검색 소장정보 검색

결과 내 검색

동의어 포함

목차보기

표제지

목차

Abstract 9

1장 둥근성게의 Smads와 Estrogen Receptor-related Receptorβ like 1 (ERRβ like 1)의 cDNA 동정 11

서론 11

재료 및 방법 14

결과 16

고찰 18

2장 계절별 및 배발생 stage별 Smad3와 ERRβ like 1 mRNA 발현 20

서론 20

재료 및 방법 22

결과 24

고찰 25

3장 estradiol-17β와 Nonylphenol이 둥근성게의 배발생동안 ERRβ like 1의 mRNA발현에 미치는 영향 27

서론 27

재료 및 방법 29

결과 30

고찰 31

그림 및 표 32

참고문헌 56

감사의 글 64

List of Table

Table 1. Oligonucleotides used for sequence determination and PCR, to obtain cDNAs encoding Smad3, smad4, Smad6, Smad8 and ERR. 32

Table 2. Comparison of Smad3 amino acid homology(%). 40

Table 3. Comparison of Smad4 amino acid homology(%). 41

Table 4. Comparison of Smad6 amino acid homology(%). 42

Table 5. Comparison of Smad8 amino acid homology(%). 43

Table 6. Comparison of ERRβ like 1 amino acid homology(%). 44

Table 7. Oligonucleotides use for sequence determination and Quantitative real-time PCR(QRCR) for Smad 3, ERRβ like 1 and Ubiquitin. 46

Table 8. Embryonic lethality(%) by estradiol-17β and nonyphenol exposure. 54

List of Figure

Fig. 1. Nucleotide and deduced amino acid sequences of the S. nudus Smad3. 33

Fig. 2. Nucleotide and deduced amino acid sequences of the S. nudus Smad4. 34

Fig. 3. Nucleotide and deduced amino acid sequences of the S. nudus Smad6. 35

Fig. 4. Nucleotide and deduced amino acid sequences of the S. nudus Smad8. 36

Fig. 5. Nucleotide and deduced amino acid sequences of the S. nudus ERRβ like 1. 37

Fig. 6. A phylogenetic tree for Smad3, Smad proteins constructed by the neighbor-joining method. 38

Fig. 7. A phylogenetic tree for ERRβ like 1 proteins constructed by the neighbor-joining method. 39

Fig. 8. Transcriptional activity of S. nudus Smad3 in 293T cell(n=3). 45

Fig. 9. Standard curves and typical amplification plot for QPCR analysis. 47

Fig. 10. Expression levels of Smad3 mRNA during seasonal change of Strongylocentrotus nudus. 48

Fig. 11. Expression levels of ERRβ like 1 mRNA during seasonal change of Strongylocentrotus nudus. 49

Fig. 12. Expression levels of Smad3 mRNA in the embryo of Strongylocentrotus nudus. 50

Fig. 13. Expression levels of ERRβ like 1 mRNA in the embryo of Strongylocentrotus nudus. 51

Fig. 14. Morphology of embryonic development in wild-type S. nudus. 52

Fig. 15. Morphology of embryonic development by 17β-estradiol and nonylphenol exposure. 53

Fig. 16. Expression levels of ERRβ like 1 mRNA by estradiol-17β(E₂) and nonylphenol(NP) exposure. 55