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목차보기

표제지 1

Abstract 3

목차 5

Ⅰ. 서언 11

Ⅱ. 연구사 14

Ⅲ. 재료 및 방법 18

1. 항바이러스 활성 검정 시료 조제 18

2. 공시바이러스 및 접종원 조제 18

3. 항바이러스 활성검정 식물 19

4. 항바이러스 활성검정 20

5. 국부감염 기주에서의 감염억제효과 21

5-1. PMMoV 감염억제효과 검정 21

5-2. CMV 감염억제효과 검정 22

6. 전신감염 기주에서의 감염억제효과 24

6-1. PMMoV 억제효과검정 24

6-2. CMV 억제효과검정 24

6-3. ZYMV 억제효과검정 24

7. 토양전염 바이러스에 대한 감염억제효과 검정 25

7-1. PMMoV 토양전염 억제효과 검정 25

7-2. CGMMV 토양전염 억제효과 시험 25

8. KN0912의 PMMoV 입자 및 PMMoV RNA에 미치는 영향 26

8-1. PMMoV의 순화 26

8-2. 전자현미경 관찰을 통한 PMMoV 순화 입자에 미치는 KN0912의 효과검정 26

8-3. PMMoV RNA의 분리 27

8-4. PMMoV RNA의 감염성에 대한 KN0912의 효과검정 27

Ⅳ. 결과 및 고찰 28

1. 약용식물체 추출물, KN0912의 바이러스감염 억제 효과 28

2. 국부감염 기주에서의 감염억제효과 29

2-1. PMMoV 억제효과검정 29

2-2. CMV 억제효과검정 33

3. 전신감염 기주에서의 감염억제효과 37

3-1. PMMoV 억제효과검정 37

3-2. CMV 억제효과검정 38

3-3. ZYMV 억제효과검정 39

4. 토양전염 바이러스에 대한 감염억제효과시험 40

4-1. PMMoV 토양전염 억제효과 시험 40

4-2. CGMMV 토양전염 억제효과 시험 41

5. KN0912의 PMMoV 입자 및 PMMoV RNA에 미치는 영향 42

5-1. 전자현미경 관찰을 통한 PMMoV 순화 입자에 미치는 KN0912의 효과검정 42

5-2. PMMoV RNA의 감염성에 대한 KN0912의 효과검정 42

Ⅴ. 결론 44

참고문헌 47

표목차 9

Table 1. Plant inhibitors against virus infection and virus... 13

Table 2. Inhibitory effects of KN0912 against PMMoV infection by... 29

Table 3. Inhibitory effect of pretreatment of KN0912 on the... 30

Table 4. Permeability effect of KN0912 on the infection of PMMoV... 31

Table 5. Persistence test of antiviral activity of KN0912 against... 32

Table 6. Inhibitory effects of KN0912 against CMV infection by... 33

Table 7. Inhibitory effect of pretreatment of KN0912 on the... 34

Table 8. Permeability effect of KN0912 on the infection of CMV to... 35

Table 9. Persistence test of antiviral activity of KN0912 against... 36

Table 10. Inhibitory activity of KN0912 against PMMoV infection... 37

Table 11. Inhibitory activity of KN0912 against CMV infection on... 38

Table 12. Inhibitory activity of KN0912 against ZYMV infection on... 39

Table 13. Inhibitory activity of KN0912 against PMMoV infection... 40

Table 14. Inhibitory activity of KN0912 against CGMMV infection... 41

Table 15. Inhibition effects from KN0912 against PMMoV infection... 42

그림목차 7

Fig. 1. Inhibitory effect of local lesion symptom induced by MeOH... 28

Fig. 2. Inhibitory effect of PMMoV and KN0912 mixture solution of... 29

Fig. 3. Pretreatment effect of KN0912 against PMMoV infection 30

Fig. 4. Permeability effect of KN0912 on PMMoV infection 31

Fig. 5. Persistence test of KN0912 on N. glutinosa 32

Fig. 6. Inhibitory effect of CMV and KN0912 mixture solution of... 33

Fig. 7. Pretreatment effect of KN0912 against CMV infection 34

Fig. 8. Permeability effect of KN0912 on CMV infection 35

Fig. 9. Persistence test of KN0912 on C. amaranticolor 36

Fig. 10. Systemic inhibitory effects of KN0912 against PMMoV... 37

Fig. 11. Systemic inhibitory effects of KN0912 against CMV... 38

Fig. 12. Soil transmission effect of KN0912 against PMMoV... 40

Fig. 13. Inhibitory effects of KN0912 against PMMoV RNA... 43

Fig. 14. Morphological changes of virus particles by a treatment of... 43

초록보기

　Many traditional medicinal plants have been reported to have strong antiviral activity and some of them have already been used to treat animals and people who suffer from viral infection.

For the development of new antiviral inhibitors from methanol extracts of Quercus dentata Thunb of gall was tested against pepper mild mottle virus by local lesion assay on Nicotiana glutinosa. An extract of gall (Quercus dentata Thunb) showed the strong activity against pepper mild mottle virus infection. Based on these results, the inhibitor named as "KN0912" formulated from extract of Quercus dentata Thunb of gall was tested for its inhibitory effects on PMMoV and CMV infection to each local lesion host plant. First of all, mixture treatment effect of KN0912 against infections of each virus (PMMoV and CMV) to local infection plant was measured to be 100% to PMMoV and 100% to CMV in 10mg/ml conc. Pretreatment effect of KN0912 against infections of each virus (PMMoV and CMV) to local infection plant was measured to be 97.9% to PMMoV and 99.4% to CMV in 10mg/ml conc. Permeability effect of KN0912 against infections of each virus (PMMoV and CMV) to local host plant was measured to be 55.7% to PMMoV and 63.8% to CMV in 10mg/ml conc. Duration of inhibitory activity of KN0912 against PMMoV infection on N. glutinosa was maintained for 3 days at 98% inhibition level and CMV infection on C. amaranticolor maintained for 3 days at 95% inhibition level. The incidence of systemic PMMoV infection was　reduced by 100% when PMMoV was inoculated onto the upper surface of leaves of N. tabacum. cv. Samsun at 24 hours after spraying of the KN0912 extract. CMV infection was inhibited by spraying the extract on N. tabacum. cv. Bright yellow by 80% when leaves were mechanically inoculated. The inhibitory effects of the extract on ZYMV infection was 90% in backchungdadagi. Soil infection inhibitory effects of PMMoV and CGMMV from pot, KN0912 was measured to be 80% to PMMoV and 100% to CGMMV in 10mg/ml conc. Observed by electron microscopy, PMMoV particles showed a finely segmented. That is to say, that inhibits the virus showed the effect.

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