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Abstract 6

서론 8

재료 및 방법 13

1. 실험 재료 13

1-1. 원료 13

1-2. 시약 13

2. 추출 14

3. 방법 14

3-1. 세포배양 14

3-2. 세포독성 측정 15

3-3. Nitric Oxide 생성량 측정 15

3-4. Pro-inflammatory cytokine 분비량 측정 16

3-5. COX-2 발현량 측정 17

4. 통계처리 18

결과 및 고찰 19

1. 세포 증식능 19

2. Nitric oxide 생성량 22

3. Pro-inflammatory cytokine 생성 억제 효과 26

3-1. TNF-α 분비량 26

3-2. IL-6 분비량 30

3-3. IL-1β 분비량 34

4. COX-2 단백질 발현 억제 효과 38

요약 41

참고문헌 43

List of Figures

Fig. 1. Effect of Sargassum fulvellum ethanol extract on the proliferation of macrophage. 20

Fig. 2. Effect of Sargassum sagamianum ethanol extract on the proliferation of macrophage. 21

Fig. 3. Effect of Sargassum fulvellum ethanol extract on nitrite production in LPS-stimulated macrophage. 24

Fig. 4. Effect of Sargassum sagamianum ethanol extract on nitrite production in LPS-stimulated macrophage. 25

Fig. 5. Effect of Sargassum fulvellum ethanol extract on TNF-α production in LPS-stimulated macrophage. 28

Fig. 6. Effect of Sargassum sagamianum ethanol extract on TNF-α production in LPS-stimulated macrophage. 29

Fig. 7. Effect of Sargassum fulvellum ethanol extract on IL-6 production in LPS-stimulated macrophage. 32

Fig. 8. Effect of Sargassum sagamianum ethanol extract on IL-6 production in LPS-stimulated macrophage. 33

Fig. 9. Effect of Sargassum fulvellum ethanol extract on IL-1β production in LPS-stimulated macrophage. 36

Fig. 10. Effect of Sargassum sagamianum ethanol extract on IL-1β production in LPS-stimulated macrophage. 37

Fig. 11. Inhibitory effects of Sargassum fulvellum ethanol extract on the protein expression of COX-2 in RAW264.7 cells. 39

Fig. 12. Inhibitory effects of Sargassum sagamianum ethanol extract on the protein expression of COX-2 in RAW264.7 cells. 40