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동의어 포함
Title Page
Contents
Abstract 6
요약 7
I. Introduction 8
II. Materials and Methods 19
1. Bacterial culture and induction treatments 19
2. Biochemical characterization of strain EB1 20
3. 16S rDNA analysis 21
4. Plant material and growth conditions 22
5. GUS histochemical assay 22
6. IAA production assay in strain EB1 23
7. Quantification of target gene expression by real-time PCR 24
8. Auxin biosynthesis inhibition assay 26
9. Plasposon random mutagenesis 27
10. Statistical analysis 28
III. Results 29
1. Strain EB1 is phyto-auxin stimulator 29
2. Strain EB1 treatment modulates root systemic architecture (RSA) of Arabidopsis 31
3. Strain EB1 belongs to genus of Enterobacter sp. 34
i) Biochemical characteristics 34
ii) 16S rDNA gene sequence analysis 37
4. Strain EB1 produces indole-3-acetic acid (IAA) 39
5. Strain EB1 promotes the growth of Arabidopsis 41
6. Strain EB1 up-regulates auxin responsive gene in Arabidopsis 43
7. Strain EB1 modulates RSA via canonical auxin signaling pathway in Arabidopsis 46
8. Phyto-auxin biosynthesis inhibitor blocked bacterial effects on root development 49
9. Mutagenesis of strain EB1 to isolate the gene regulating the expression of DR5 52
IV. Discussion 54
1. Auxin might be the key factor on strain EB1-triggered Arabidopsis growth and developmental responses 54
2. Enterobacter sp. EB1 modulates the Arabidopsis auxin signaling via ARF7 and ARF19, gate-keepers of canonical auxin signaling pathway 55
3. Enterobacter sp. EB1 induces auxin signal in Arabidopsis 55
V. References 57
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