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Two isolates of Tricholoma matsutake T-008 and T-034, preserved in Entomopathogenic Fungal Culture Collection (EFCC)
of Korea, were used in the present study. The isolates had 100% Bootstrap homology with Tricholoma matsutake U62964
and T. matsutake AB188557 and AF309538 preserved in Gene Bank of NCBI. Mycelial growth of T. matsutake was highest
in TMM and MYA at 25oC. The highest dry wt. of mycelium was obtained after 65 days of culture, when 6 mycelial discs
were inoculated in 100 ml of broth in 250 ml shaking flask. Mycelial mats were observed in clumped condition at the inoculation
sites of pine forest after two weeks of inoculation. After 5 months of inoculation, mycelia mats were observed growing
inside soil and walls of a few inoculation sites, while mycelial mats growth up to 5~8 cm were observed in the roots of pine
tree after 6 months. The survival rate of the inoculum was about 40% of the total inoculation sites. The survival rate was
found below 20% when the mycelium was inoculated in the summer. The reasons for low survival rates of the mycelium
were mainly due to dry season and the soil-borne small animals such as earthworm and mole. After one year of inoculation,
no external difference was observed between the artificially inoculated mycelia and the naturally existing mycelia of T. matsutake.
The present study showed that fruiting bodies of T. matsutake could be produced by artificial inoculation under the
appropriate environmental conditions.권호기사
참고문헌 (26건) : 자료제공( 네이버학술정보 )
| 번호 | 참고문헌 | 국회도서관 소장유무 |
|---|---|---|
| 1 | (2004) Phylogeography and evolution in matsutake and close allies inferred by analyses of ITS sequences and AFLPs, | 미소장 |
| 2 | (1995) A relationship between climatic factors and matsutake production in 29 sites during a 10-year period in Korea, | 미소장 |
| 3 | (1999) Tricholoma matsutake - an assessment of in situ and in vitro infection by observing cleared and stained whole roots, | 미소장 |
| 4 | (2005) Gentic mosaics in the massive persisting rhizosphere colony ？shiro?of the ectomycorrhizal basidiomycete Tricholoma matsutake, | 미소장 |
| 5 | (2001) Characteristics of the amylase and its related enzymes produced by ectomycorrhizal fungus Tricholoma matsutake, | 미소장 |
| 6 | C. H. 1989. Studies on analyzing meteorological elements related with yield of Tricholoma matsutake, | 미소장 |
| 7 | (pinemushroom) conidia to cultural media environment. J. Kor. For. Soc. 64, 33-41. | 미소장 |
| 8 | (2005) Favorable condition for mycelial growth of Tricholoma matsutake, | 미소장 |
| 9 | (1998) Financial analysis of vegetation control for sustainable production of Songyi (Tricholoma matsutake) in Korea, | 미소장 |
| 10 | (1997) Effect of carbon sources and vitamins on mycelial growth of Tricholoma matsutake DGUM 26001, | 미소장 |
| 11 | (1998) The extracellular enzyme activities in culture broth of Tricholoma matsutake, | 미소장 |
| 12 | K. S. 1986. A comparative study on the mushroom populations between matsutake-producing and non-producing Pinus densiflora stands. J. Kor. For. Soc. 72, | 미소장 |
| 13 | Biology of Tricholoma matsutake found at Pinus densiflora communities in the areas of Kyoung Sang Do | 소장 |
| 14 | (1998) The 18s rDNA sequences of the basidiocarps of Tricholoma matsutake in Korea, | 미소장 |
| 15 | (1999) The specific probes confirming the genomic DNA of Tricholoma matsutake in Korea, | 미소장 |
| 16 | (2003) Development of microsatellite markers from an ectomycorrhizal fungus, Tricholoma matsutake, by an ISSR-suppression-PCR method, | 미소장 |
| 17 | (1998) Effect of complex nitrogen source on mycelial growth of Tricholoma matsutake DGUM 26001, | 미소장 |
| 18 | Survey on the Flora and Main Wild Mushroom in Tricholoma matsutake Producing Sites | 소장 |
| 19 | K. W. 1980. Studies on the artificial cultivation of Armillaria matsutake Ito et Imai. J. Kor. For. Soc. No. 50, | 미소장 |
| 20 | (1995) Effects of climatic condition in September on pine-mushroom (Tricholoma matsutake) yield and a method for overcoming the limiting factors in Korea, | 미소장 |
| 21 | (1997) Effects on the pine mushroom yield of controlling environmental conditions at the pine stands in Namwon, Korea, | 미소장 |
| 22 | (1998) Ectomycorrhizal mushroom occurrence around the fairy ring of Tricholoma matsutake at a pine-mushroom forest, | 미소장 |
| 23 | S. K. 1980. A study on multiplication of Tricholoma matsutake. Kor. J. Mycol. 8, | 미소장 |
| 24 | Microfungal flora of Tricholoma matsutake producing and nonproducing sites in the forest of Pinus densiflora | 소장 |
| 25 | (2002) Saprobic potential of Tricholoma matsutake: growth over pine bark treated with surfactants, | 미소장 |
| 26 | (2001) Cultivation of mushrooms of edible ectomycorrhizal fungi associated with Pinus densiflora by in vitro mycorrhizal synthesis, | 미소장 |
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