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The effect of fluidic conditions on the bioluminescent detection of ATP in a microfluidic device was surveyed using home-made detector system. The bioluminescent reaction of ATP was directly affected by the retention time and flow rates of the solutions in this diffusion-based mixing and reaction system due to the laminar flow in the microchannel. ATP and enzyme solutions were separately injected into the microfluidic device at different flow rates through 3 inlet ports. The ATP solution was injected through the middle port, while the enzyme solution was injected in the two remaining ports. When the ratio of ATP to enzyme solution was fixed, the optimum flow rates of enzyme, ATP, and enzyme solution was 3.5, 8.0, and 3.5 μL/min, respectively. The optimal total flow rate was 15 μL/min. The detection limit for the concentration of ATP at optimal conditions was less than 10-7 M.

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기사명 저자명 페이지 원문 목차
Heparin/polypyrrole(PPy) composite on gold-coated matrix for the neurite outgrowth of PC12 cells by electrical stimulation Keun-hong Park ;Eun Ae Jo ;Kun Na pp.463-469

The effect of fluidic conditions on the continuous-flow bioluminescent detection of ATP in a microfluidic device Thuan-hieu Tran ;Woo-jin Chang ;Young-bum Kim ;Yoon-mo Koo ;Eun-ki Kim ;Joo-young Yoon ;Jinhwan Kim pp.470-474

Influence of pH and impeller tip speed on the cultivation of Bifidobacterium pseudocatenulatum G4 in a milk-based medium W. Stephenie ;B. M. Kabeir ;M. Shuhaimi ;M. Rosfarizan ;A. M. Yazid pp.475-483

Lipase-catalyzed hydrolysis of fish oil in an optimum emulsion system Hee-guk Byun ;Tae-kil Eom ;Won-kyo Jung ;Se-kwon Kim pp.484-490

Lipase catalyzed production of monoacylglycerols by the esterification of fish oil fatty acids with glycerol Hee-guk Byun ;Tae-kil Eom ;Won-kyo Jung ;Se-kwon Kim pp.491-496

Application of sodium propionate to the suspension culture of Chinese hamster ovary cells for enhanced production of follicle-stimulating hormone Sung Kwan Yoon ;Yong-ho Ahn pp.497-501

Enhanced tolerance against freezing stress in Escherichia coli cells expressing an algal cyclophilin gene Eun Kyung Cho pp.502-507

Cultural characteristics and extraction of the fungal pigment phleichrome from the phytopathogenic fungus Cladosporium phlei Joong-keun Lee ;Beom-tae Kim ;Jung-ae Kim ;Hea-jong Chung ;Seung-moon Park ;Moon-sik Yang ;Ki-jun Hw pp.508-515

Effects of Fomitopsis pinicola extracts on antioxidant and antitumor activities Dubok Choi ;Sang-shin Park ;Ji-lu Ding ;Wol-suk Cha pp.516-524

Effect of mobile phase additives on resolution of some nucleic compounds in high performance liquid chromatography Chun Hua Jin ;Yoon Mo Koo ;Dae-ki Choi ;Kyung Ho Row pp.525-530

Effect of scale-up and seasonal variation on biokinetics in the enhanced bioremediation of petroleum hydrocarbon-contaminated soil Ilwon Ko ;Kyoung-woong Kim ;Cheol-hyo Lee ;Kwang-pyo Lee pp.531-541

Solubilization conditions for hydrophobic membrane protein, oleosin, in soybeans Hyun Kim ;So-young Kim ;Nam Soo Han ;Bernard Y. Tao pp.542-547

Optimization of medium for the production of a novel aquaculture probiotic, Micrococcus MCCB 104 using central composite design R. Preetha ;N. S. Jayaprakash ;Rosamma Philip ;I. S. Bright Singh pp.548-555

Effects of temperature shift strategies on human preproinsulin production in the fed-batch fermentation of recombinant Escherichia coli Young-jin Son ;Kyong-hee Park ;Sang-yong Lee ;Sung-jin Oh ;Chang-kyu Kim ;Byoung-taek Choi ;Yong-che pp.556-561

Preparation of template plasmids for cell-free protein synthesis using a regenerated anion-exchange column Kyeong-ohn Kim ;Ju-young Byun ;Dong-myung Kim pp.562-565

Development of a serial bioreactor system for direct ethanol production from starch using Aspergillus niger and Saccharomyces cerevisiae Bo Young Jeon ;Soo Jin Kim ;Dae Hee Kim ;Byung Kwan Na ;Doo Hyun Park ;Hung Thuan Tran ;Ruihong Zhan pp.566-573

Use of L-buthionine sulfoximine for the efficient expression of disulfide-containing proteins in cell-free extracts of Escherichia coli In-seok Oh ;Tae-wan Kim ;Jin-ho Ahn ;Jung-won Keum ;Cha-yong Choi ;Dong-myung Kim pp.574-578

Antibacterial activity of Ulva lactuca against methicillin-resistant Staphylococcus aureus(MRSA) In Hae Kim ;Dong-gun Lee ;Sang Hyun Lee ;Jong-myung Ha ;Bae-jin Ha ;Sung-koo Kim ;Jae-hwa Lee pp.579-582

참고문헌 (20건) : 자료제공( 네이버학술정보 )

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번호 참고문헌 국회도서관 소장유무
1 Duffy D. C, (1998) Rapid prototyping of microfluidicsystems in poly(dimethylsiloxane), 미소장
2 Stachowiak T. B, (2003) Fabricationof porous polymer monoliths covalently attached to thewalls of channels in plastic microdevices, 미소장
3 Li C, (2005) Isoelectric focusing in cyclic olefin copolymer microfluidic channels coated by polyacrylamide using a UV photograftingmethod, 미소장
4 Sohn, Y.-S, (2004) Protein arraypatterning on cyclic olefin copolymer (COC) for disposableprotein chip, 미소장
5 Weigl, B. H, (2003) Lab-on-a-chip for drug development, 미소장
6 New York, (1992) BioluminescenceAnalysis, 미소장
7 Ahn J.-M, (2006) Characterizationof gltA::luxCDABE fusion in Escherichia coli asa toxicity biosensor, 미소장
8 Min, J, (2007) 17β-Estradiol-stimulated eNOS genetranscriptional activation is regulated through the estrogen-responsive element in eNOS promoter, 미소장
9 Girotti S, (1997) Luminescenttechniques applied to bioanalysis, 미소장
10 Liu B.-F, (2005) Microfluidic chip towardcellular ATP and ATP-conjugated metabolicanalysis with bioluminescence detection, 미소장
11 Kim Y.-B, (2006) Statistical optimization ofthe lysis agents for gram-negative bacterial cells in a microfluidicdevice, 미소장
12 Tsukagoshi, K, (2005) Capillary electrophoresis apparatus equipped with a bioluminescencedetector using a batch- or flow-type detectioncell, 미소장
13 Kirner, T, (2004) Spatiallyresolved detection of miniaturized reactiondiffusionexperiments in chip reactors for educationalpurposes, 미소장
14 Baroud C. N, (2003) Reaction-diffusion dynamics: confrontation betweentheory and experiment in a microfluidic reactor, 미소장
15 Effenhauser C. S, (1997) Integrated capillary electrophoresis onflexible silicone microdevices: analysis of DNA restrictionfragments and detection of single DNA moleculeson microchips, 미소장
16 Whitesides G. M, (2001) Soft lithography in biology andbiochemistry, 미소장
17 Bishop, R. H, (2001) LabVIEW Student Edition 6i, PrenticeHall 미소장
18 Salama K, (2004) Modeling and simulation of luminescence detectionplatforms, 미소장
19 Gomi, K. , (2001) Oxyluciferin, a luminescenceproduct of firefly luciferase, is enzymaticallyregenerated into luciferin, 미소장
20 Wood, (1985) Cloning of firefly luciferase cDNA andthe expression of active luciferase in Escherichia coli, 미소장

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