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Soybean ( Glycine max L.) Kunitz trypsin inhibitor (KTI) protein is an antinutritional factor. The

objective of this research was to confirm a SSR marker tightly linked to the Ti locus in four different

F5 populations. Four female parents ( Glycine max L. cv. Jinpumkong2, Hannamkong, GS06,

20M183) of TiTi (KTI protein present) genotype and one male parent C242 of titi (KTI protein

absent) genotype were used. Four different populations of F2 plants free of KTI protein were

advanced to the F5 generation. Satt228 marker analysis showed the four female parents had the

allele 1 (AA genotype) while the C242 male parent has the allele 2 (BB genotype). In seed, four

parents had KTI protein and C242 had not KTI protein of 21.5 kDa. A total of 391 F5 plants derived

from the four crosses (273 plants from Jinpunkong2 ?C242, 17 plants from Hannamkong ?C242,

45 plants from GS06 ?C242, and 56 plants from 20M183 ?C242) all have the allele 2 (BB genotype)

for Satt228 marker. The 391 individual F6 seeds harvested from same individual F5 plants are also

absent of the KTI protein. Complete cosegregation between the Satt228 marker allele and the Ti

locus was observed in these four different populations.

권호기사

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참고문헌 (27건) : 자료제공( 네이버학술정보 )

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번호 참고문헌 국회도서관 소장유무
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