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An amperometric glucose biosensor was designed for the detection of glucose in blood, urine, beverages, and fermentation systems. In typical glucose biosensors that employ enzymes, mediators are used for efficient electron transfer between the enzymes and the electrode. However, some of these mediators are known to be toxic to the enzymes and also must be immobilized on the surface of the electrode. We propose a mediator-free glucose biosensor that uses a glucose oxidase immobilized on a tin oxide electrode. Direct electron transfer is possible in this system because the tin oxide has redox properties similar to those of mediators. The method for immobilization of the glucose oxidase onto the tin oxide is also very simple. Tin oxide was prepared by the anodization and annealing of pure tin, and this provides a large surface area for the immobilization step because of its porosity. Glucose oxidase was immobilized onto the tin oxide using the membrane entrapment method. The proposed method provides a simple process for fabricating the enzyme electrode. Glucose oxidase immobilized onto the tin oxide, prepared in accordance with this method, has a relatively large current response when compared to those of other glucose biosensors. The sensitivity of the biosensor was 19.55 μA/mM, and a linear response was observed between 0~3 mM glucose. This biosensor demonstrated good reproducibility and stability.

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Amperometric determination of glucose, based on the direct electron transfer between glucose oxidase and tin oxide Jae Yeon Park ;Yang Hee Kim ;Andrew Seong ;Young Je Yoo pp.431-435

Identification and characterization of the bacterial D-gluconate dehydratase in achromobacter xylosoxidans Seonghun Kim ;Sun Bok Lee pp.436-444

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참고문헌 (20건) : 자료제공( 네이버학술정보 )

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번호 참고문헌 국회도서관 소장유무
1 Wang, J., (2008) Electrochemical glucose biosensors, Chem. Rev 미소장
2 Pickup, J., (1993) Developing glucose sensors for in vivo use, Trends Biotechnol 미소장
3 Hecht, H. J., (1993) Crystal structure of glucose oxidase from Aspergillus niger refined at 2·3 Å resolution, J. Mol. Biol 미소장
4 Luo, X., (2006) Reagentless glucose biosensor based on the direct electrochemistry of glucose oxidase on carbon nanotubemodified electrodes, Electroanalysis 미소장
5 Ghica, M.E. , (2005) A glucose biosensor using methyl viologen redox mediator on carbon film electrodes, Anal. Chim. Acta 미소장
6 Liu, X., (2004) Enzymatic activity of glucose oxidase covalently wired via viologen to electrically conductive polypyrrole films, Biosens. Bioelectron 미소장
7 유영제 ; Seung Hwan Kim ; Seung Hoon Song, (2005) Selection of Mediators for Bioelectrochemical Nitrate Reduction, Biotechnology and Bioprocess Engineering 미소장
8 Hendry, S. P., (1993) Polyferrocenes as mediators in amperometric biosensors for glucose, Anal. Chim. Acta 미소장
9 김학성 ; Hyun C. Yoon, (2004) Bioelectrocatalyzed Signal Amplification for Affinity Interactions at Chemically Modified Electrodes, Biotechnology and Bioprocess Engineering 미소장
10 Shan, D., (2007) Electrochemical study of ferrocenemethanol-modified layered double hydroxides composite matrix: Application to glucose amperometric biosensor, Biosens. Bioelectron 미소장
11 Kotzian, P., (2006) Amperometric glucose biosensor based on rhodium dioxide-modified carbon ink, Electroanalysis 미소장
12 Warren, S., (2005) Investigation of novel mediators for a glucose biosensor based on metal picolinate complexes, Bioelectrochemistry 미소장
13 Liu, Y., (2005) The direct electron transfer of glucose oxidase and glucose biosensor based on carbon nanotubes/chitosan matrix, Biosens. Bioelectron 미소장
14 Pan, D., (2005) An amperometric glucose biosensor based on glucose oxidase immobilized in electropolymerized poly(o-aminophenol) and carbon nanotubes composite film on a gold electrode, Anal. Sci 미소장
15 Zhang, F., (2006) Gold nanoparticle-based mediatorless biosensor prepared on microporous electrode, Electroanalysis 미소장
16 Wu, J., (2005) Electrochemical performances of C/Fe nanocomposite and its use for mediator-free glucose biosensor preparation, Talanta 미소장
17 Salimi, A., (2007) Immobilization of glucose oxidase on electrodeposited nickel oxide nanoparticles: Direct electron transfer and electrocatalytic activity, Biosens. Bioelectron 미소장
18 Choi, H. N., (2005) Amperometric glucose biosensor based on sol-gel-derived metal oxide/Nafion composite films, Anal. Chim. Acta 미소장
19 Shin, H.-C., (2004) Porous tin oxides prepared using an anodic oxidation process, Adv. Mater 미소장
20 Laviron, E., (1979) General expression of the linear potential sweep voltammogram in the case of diffusionless electrochemical systems, J. Electroanal. Chem 미소장

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