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The present study investigated the antiproliferative effect of Litsea japonica in HL-60/ADR, adriamycin resistant
human promyelocytic leukemia cells. The 80 % ethanol extract of L. japonica markedly inhibited the growth of HL-60/ADR
cells. When HL-60/ADR cells were treated with the extract, several apoptosis events like as DNA fragmentation, chromatin
condensation and the increase of the population of sub-G1 hypodiploid cells were observed. In the mechanism of apoptosis
induction by L. japonica, we examined the changes of Bcl-2 and Bax protein expression levels, and activation of caspases. After
the HL-60/ADR cells were treated with the extract, the Bcl-2 expression was decreased, whereas the expression of Bax was
increased in a time-dependent manner compared to the control. In addition, the active forms of caspase-9 and -3 were increased
and the cleavage of poly (ADP-ribose) polymerase, a vital substrate of effector caspase, was observed. The results suggest that
the inhibitory effect of L. japonica on the growth of the HL-60/ADR appears to arise from the induction of apoptosis via the
down-regulation of Bcl-2 and the activation of caspases.| 번호 | 참고문헌 | 국회도서관 소장유무 |
|---|---|---|
| 1 | Decreased accumulation of [14c]carboplatin in human cisplatin‐resistant cells results from reduced energy‐dependent uptake ![]() |
미소장 |
| 2 | Multidrug resistance in cancer: role of ATP-dependent transporters. ![]() |
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| 3 | Modulators and substrates of P-glycoprotein and cytochrome P4503A coordinately up-regulate these proteins in human colon carcinoma cells. ![]() |
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| 4 | Significance of Nuclear Glutathione S‐Transferase π in Resistance to Anti‐cancer Drugs ![]() |
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| 5 | Characterization of a novel topoisomerase I mutation from a camptothecin-resistant human prostate cancer cell line. ![]() |
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| 6 | Butanolides from Litsea japonica ![]() |
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| 7 | Components of the Lauraceae family—I ![]() |
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| 8 | Lactones from the leaves of Litsea japonica and their anti-complement activity. ![]() |
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| 9 | Flavonoids from the leaves of Litsea japonica and their anti‐complement activity ![]() |
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| 10 | Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing. ![]() |
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| 11 | Initiation of Apoptosis versus Necrosis by Photodynamic Therapy with Chloroaluminum Phthalocyanine ![]() |
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| 12 | A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding ![]() |
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| 13 | The Effect of Litsea japonica on the Apoptosis Induction of HL-60 Leukemia Cells | 소장 |
| 14 | Mitochondrial control of apoptosis ![]() |
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도서위치안내: 정기간행물실(524호) / 서가번호: 국내06
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