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Acinetobacter baumannii BD5 was isolated from waters of

Baek-du mountain, and the lipase gene was cloned using a

PCR technique. The deduced amino acid sequence of the

lipase and lipase chaperone were found to encode proteins

of 325 aa and 344 aa with a molecular mass of 35 kDa and

37 kDa, respectively. The lipase gene was cloned and

expressed in Escherichia coli BL21 (trxB) as an inclusion

body, which was subsequently solubilized by urea, and

then purified using Ni-affinity chromatography. After being

purified, the lipase was refolded by incubation at 4oC in the

presence of a 1:10 molar ratio of lipase:chaperone. The

maximal activity of the refolded lipase was observed at a

temperature of 35oC and pH 8.3 when p-NP caprate (C10)

was used as a substrate; however, 28% of the activity

observed at 35oC was still remaining at 0oC. The stability of

the purified enzyme at low temperatures indicates that it is

a cold-adapted enzyme. The refolded lipase was activated

by Ca2+, Mg2+, and Mn2+, whereas Zn2+ and Cu2+ inhibited

it. Additionally, 0.1% Tween 20 increased the lipase

activity by 33%, but SDS and Triton X-100 inhibited the

lipase activity by 40% and 70%, respectively.

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기사명 저자명 페이지 원문 목차
Genetic and phenotypic diversity of fenitrothion-degrading bacteria isolated from soils Kim, Kyung-Duk ;Jae-Hyung Ahn ;Taesung Kim ;Seong Chan Park ;Chi Nam Seong ;Hong-Gyu Song ;Jong-Ok K pp.113-120

Identification and functional characterization of an afsR homolog regulatory gene from Streptomyces venezuelae ATCC 15439 Maharjan, Sushila ;Tae-Jin Oh ;Hei Chan Lee ;Jae Kyung Sohng pp.121-127

Gene cloning, purification, and characterization of a cold-adapted lipase produced by Acinetobacter baumannii BD5 Park, In-Hye ;Sun-Hee Kim ;Yong-Seok Lee ;Sang-Cheol Lee ;Yi Zhou ;Cheol-Min Kim ;Soon-Cheol Ahn ;Yo pp.128-135

Functional expression of SAV3818, a putative TetR-family transcriptional regulatory gene from Streptomyces avermitilis, stimulates antibiotic production in Streptomyces species Duong, Cac Thi Phung ;Han-Na Lee ;Si-Sun Choi ;Sang Yup Lee ;Eung-Soo Kim pp.136-139

Development of an analysis program of type I polyketide synthase gene clusters using homology search and profile hidden Markov model Tae, Hongseok ;Jae Kyung Sohng ;Kiejung Park pp.140-146

Gene cloning, expression, and characterization of a new carboxylesterase from Serratia sp. SES-01 :comparison with Escherichia coli BioHe enzyme Kwon, Min-A ;Hyun Suk Kim ;Joon Young Oh ;Bong Keun Song ;Jae Kwang Song pp.147-154

Kaempferol isolated from Nelumbo nucifera stamens negatively regulates FcεRI expression in human basophilic KU812F cells Shim, Sun-Yup ;Jae-Sue Choi ;Dae-Seok Byun pp.155-160

Hydrothermal acid pretreatment of Chlamydomonas reinhardtii biomass for ethanol production Nguyen, Minh Thu ;Seung Phill Choi ;Jinwon Lee ;Jae Hwa Lee ;Sang Jun Sim pp.161-166

Optimization and scale-up of succinic acid production by Mannheimia succiniciproducens LPK7 Oh, In Jae ;Dong Hyun Kim ;Eun Kyoung Oh ;Sang Yup Lee ;Jinwon Lee pp.167-171

Cloning of a gene encoding dextranase from Lipomyces starkeyi and its expression in Pichia pastoris Kang, Hee-Kyoung ;Ji-Young Park ;Joon-Seob Ahn ;Seung-Heuk Kim ;Doman Kim pp.172-177

Screening and characterization of probiotic lactic acid bacteria isolated from Korean fermented foods Lim, Sung-Mee ;Dong-Soon Im pp.178-186

Identification of novel esterase from metagenomic library of Yangtze River Wu, Chao ;Baolin Sun pp.187-193

Expression of HpaG_Xooc_ protein in Bacillus subtilis and its biological functions Wu, Huijun ;Shuai Wang ;Junqing Qiao ;Jun Liu ;Jiang Zhan ;Xuewen Gao pp.194-203

Antibacterial and synergistic activity of isocryptomerin isolated from Selaginella tamariscina Lee, Juneyoung ;Yunjung Choi ;Eun-Rhan Woo ;Dong Gun Lee pp.204-207

Differential expression of nuclear receptors in T helper cells Hwang, Soo Suk ;Young Uk Kim ;Wonyong Lee ;Gap Ryol Lee pp.208214

참고문헌 (28건) : 자료제공( 네이버학술정보 )

참고문헌 목록에 대한 테이블로 번호, 참고문헌, 국회도서관 소장유무로 구성되어 있습니다.
번호 참고문헌 국회도서관 소장유무
1 Alquati, C., L. De Gioia, G. Santarossa, L. Alberghina, P. Fantucci, and M. Lotti. 2002. The cold-active lipase of Pseudomonas fragi. Eur. J. Biochem. 269: 3321-3328. 미소장
2 Bacterial lipolytic enzymes: classification and properties 네이버 미소장
3 Prospecting for novel lipase genes using PCR. 네이버 미소장
4 Breuil, C. and D. J. Kushner. 1974. Partial purification and characterization of the lipase of a facultatively psychrophilic bacterium (Acinetobacter O16). Can. J. Microbiol. 21: 434-441. 미소장
5 Choo, D. W., T. Kurihara, T. Suzuki, K. Soda, and N. Esaki. 1998. A cold-adapted lipase of an alaskan psychrotroph, Pseudomonas sp. strain B11-1: Gene cloning and enzyme purification and characterization. Appl. Environ. Microbiol. 64: 486-491. 미소장
6 Expression and characterization of a novel enantioselective lipase from Acinetobacter species SY-01 네이버 미소장
7 Hong, M. C. and M. C. Chang. 1998. Purification and characterization of an alkaline lipase from a newly isolated Acinetobacter radioresistens CMC-1. Biotechnol. Lett. 20: 1027-1029. 미소장
8 Bacterial biocatalysts: molecular biology, three-dimensional structures, and biotechnological applications of lipases. 네이버 미소장
9 Biotechnology-overexposed? 네이버 미소장
10 Sequence-based approach to finding functional lipases from microbial genome databases 네이버 미소장
11 Biotechnological studies in the Far-Eastern Region of Russia. 네이버 미소장
12 A Novel Lipase from Pseudomonas fluorescens HU380: Gene Cloning, Overproduction, Renaturation-Activation, Two-Step Purification, and Characterization 네이버 미소장
13 Characterization of the extracellular lipase, LipA, of Acinetobacter calcoaceticus BD413 and sequence analysis of the cloned structural gene. 네이버 미소장
14 Characterization of a Novel DNA Minor-Groove Complex 네이버 미소장
15 Kumar, S., K. Kikon, A. Upadhyay, S. S. Kanwar, and R. Gupta. 2005. Production, purification, and characterization of lipase from thermophilic and alkaliphilic Bacillus coagulans BTS-3. Protein Expr. Purif. 41: 38-44. 미소장
16 Biotechnology and bioeconomy in China. 네이버 미소장
17 Quyen, D. T., T T. G. Le, T. T. Nguyen, T. K. Oh, and J. K. Lee. 2005. High-level heterologous expression and properties of a novel lipase from Ralstonia sp. M1. Protein Expr. Purif. 39: 97-106. 미소장
18 Quyen, D. T., T. T. Nguyen, T. T. G. Le, H. K. Kim, T. K. Oh, and J. K. Lee. 2004. A novel lipase/chaperone pair from Ralstonia sp. M1: Analysis of the folding interaction and evidence for gene loss in R. solanacearum. Mol. Gen. Genomics 272: 538-549. 미소장
19 Rashid, N., Y. Shimada, S. Ezaki, H. Atomi, and T. Imanaka. 2001. Low-temperature lipase from psychrotrophic Pseudomonas sp. strain KB700A. Appl. Environ. Microbiol. 67: 4064-4069. 미소장
20 Biotechnology and bioeconomy in China. 네이버 미소장
21 Schlieben, N. H., K. Niefind, and D. Schomburg. 2004. Expression, purification, and aggregation studies of His-tagged thermoalkalophilic lipase from Bacillus thermocatenulatus. Protein Expr. Purif. 34: 103-110. 미소장
22 Snellman, E. A. and R. R. Colwell. 2004. Acinetobacter lipases: Molecular biology, biochemical properties and biotechnological potential. J. Ind. Microbiol. Biotechnol. 31: 391-400. 미소장
23 Purification and properties of the extracellular lipase, LipA, of Acinetobacter sp. RAG-1. 네이버 미소장
24 Cloning and sequence analysis of the lipase and lipase chaperone-encoding genes from Acinetobacter calcoaceticus RAG-1, and redefinition of a Proteobacterial lipase family and an analogous lipase chaperone family 네이버 미소장
25 Suzuki, T., T. Nakayama, D. W. Choo, Y. Hirano, T. Kurihara, T. Nishino, and N. Esaki. 2003. Cloning, heterologous expression, renaturation, and characterization of a cold-adapted esterase with unique primary structure from a psychrotroph Pseudomonas sp. strain B11-1. Protein Expr. Purif. 30: 171-178. 미소장
26 Cold-Active Lipolytic Activity of Psychrotrophic Acinetobacter sp. Strain No. 6. 네이버 미소장
27 Tang, S. J., K. H. Sun, G. H. Sun, T. Y. Chang, and G. C. Lee. 2000. Recombinant expression of the Candida rugosa lip4 lipase in Escherichia coli. Protein Expr. Purif. 20: 308-313. 미소장
28 Biotechnological studies in the Far-Eastern Region of Russia. 네이버 미소장

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