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동의어 포함
This paper aimed to develop a solvent extraction and purification process to
recover high-purified β-carotene from recombinant Escherichia coli. Cells harvested from the culture broth were treated through numerous steps: dehydration, solvent extraction, crystal formation and separation. To
optimize the extracting condition, experiments were carried out to investigate the effect of cell disruption, temperature, organic solvents, solvent-biomass ratio on the yield of β-carotene extracted from cells. The result
indicated that no significant differences of extraction yield were observed from cells with or without step of cell disruption. Among different extracting solvents, the highest extraction yield of β-carotene, 30.3 mg-β-carotene/
g-dry cells, was obtained with isobutyl acetate at solvent-biomass ratio 25 mL/g-dry cells at 50℃. Notably, in case of acetone, the extraction yield was quite low when using acetone itself, but increased almost up to
the highest value when combining this solvent and olive oil. The purity of β-carotene crystals obtained from crystallization and separation was 89%. The purity degree was further improved up to 98.5% by treating crude
crystals with additional ethanol washing.*표시는 필수 입력사항입니다.
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도서위치안내: 정기간행물실(524호) / 서가번호: 국내12
2021년 이전 정기간행물은 온라인 신청(원문 구축 자료는 원문 이용)
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