본문 바로가기 주메뉴 바로가기
22대 대한민국 국회 국회정보길라잡이 국회의원검색
회원가입 로그인
HOME

정보검색

소장정보 검색

국회도서관 홈으로 정보검색 소장정보 검색

결과 내 검색

동의어 포함

고급검색

인명/단체명

저자정보 상세정보
인명/단체명을 입력하세요.

키워드

  • 대표어

  • 외국어

-

목차보기

Contents

Development of Microsatellite Markers to Distinguish South Korean and Chinese ginseng / Chang-Ho Ahn ; Boo-Bae Kim ; Eui-Soo Yoon ; Yong-Eui Choi 1

Abstract 1

Introduction 1

Materials and Methods 2

1. Plant materials and extraction of genomic DNA 2

2. Construction of a microsatellite-enriched library from Korean wild ginseng 2

3. Library screen 3

4. SSR-specific primer design and PCR amplification 3

5. Genotyping of the microsatellites 3

6. Electrophoresis and silver staining 3

Results and Discussion 3

1. Development of SSR markers 3

2. Authentication of Korean ginseng from Chinese ginseng 4

3. Analysis in Yangyang-gun samples 7

Acknowledgements 7

Literature Cited 7

초록보기

Korean wild and forest cultivated ginseng has long been accepted as high medicinal values compared to field cultivated ginseng. Owing to the high price of Korean wild ginseng, Chinese wild and forest cultivated ginseng were smuggled and sold as Korean wild and forest cultivated ginseng. Therefore, an efficient method is required to distinguish Korean ginseng from Chinese ginseng. Microsatellites, simple sequence repeats (SSRs), are highly polymorphic loci present in DNA that consist of repeating units of base pairs. Thus SSR markers are highly advantageous for detection of small genetic variances of intra-species. In the present study, we constructed a microsatellite-enriched genomic library from South Korean wild Panax ginseng. After sequence analysis of 992 randomly picked positive colonies, 126 (12.7%) of the colonies were found to contain microsatellite sequences, and 38 primer pairs were designed. By polymorphism assessment using 36 primer pairs, 4 primers (PG409, PG450, PG491, and PG582) were shown to be polymorphic to distinguish the South Korean ginseng from the Chinese ginseng. These 4 microsatellite markers will provide powerful tools to authenticate South Korean ginseng from Chinese ginseng.

권호기사

권호기사 목록 테이블로 기사명, 저자명, 페이지, 원문, 기사목차 순으로 되어있습니다.
기사명 저자명 페이지 원문 목차
Researches on Populus in Korea for various purposes Chul-Woo Kim ;Du-Song Cha ;Yong-Eui Choi ;Yeong-Bon Koo ;Wan-Yong Choi ;Jae-Heon Oh ;Jae-Seon Yi pp.513-523

보기
The importance of using correct names in taxonomy :a case study of "The Genera of Vascular Plants of Korea" and other recent published literature in Korea Kae Sun Chang ;Heung Soo Lee ;Chin-Sung Chang pp.524-530

보기
A preliminary population genetic study of an overlooked endemic ash, Fraxinus chiisanensis in Korea using allozyme variation Heung Soo Lee ;Chin-Sung Chang ;Hui Kim ;Do Yeol Choi pp.531-538

보기
Biodiversity, spore density and root colonization of arbuscular mycorrhizal fungi at expressway cut-slopes in Korea Kyung Joon Lee ;Kyu Hwa Lee ;Evangeline Tamolang-Castillo ;Sri Wilarso Budi pp.539-547

보기
Households' characteristics, forest resources dependency and forest availability in central Terai of Nepal Menaka Panta ;Kyehyun Kim ;Cholyoung Lee pp.548-557

보기
Growth, carbon and nitrogen status of container grown black pine (Pinus thunbergii) seedlings at various levels of foliar fertilization Choonsig Kim ;Jaeyeob Jeong ;Tae-Shik Moon ;Jun-Ho Park ;Jong-Taek Lim ;Jong-Ik Kim ;Gwan-Hyo Goo pp.558-562

보기
Differences in bird communities between before and after forest fire in tropical dry Dipterocarp forest of the Northeastern Cambodia Shin-Jae Rhim ;Seung Hun Son ;Eun Jae Lee ;Woo-Shin Lee ;Bunnat Pech ;Masphal Kry pp.563-567

보기
Development of microsatellite markers to distinguish South Korean and Chinese ginseng Chang-Ho Ahn ;Boo-Bae Kim ;Eui-Soo Yoon ;Yong-Eui Choi pp.568-575

보기
Estimation model and vertical distribution of leaf biomass in Pinus sylvestris var. mongolica plantations Zhaogang Liu ;Guangze Jin ;Ji Hong Kim pp.576-583

보기
Impact of pruning intensity on tree growth and closure of pruning wounds of Pinus strobus L. and Acer palmatum Thunb Kyu Hwa Lee ;Kyung Joon Lee pp.584-592

보기
Changes in forest disturbance patterns from 1976 to 2005 in South Korea Pil Sun Park ;Kyu Hwa Lee ;Mun Ho Jung ;Hanna Shin ;Woongsoon Jang ;Kikang Bae ;Jongkoo Lee ;Don Koo pp.593-601

보기
Cadmium accumulation, phosphorus concentration and growth response of Cd-treated ectomycorrhizal poplar cuttings Sim-Hee Han ;Du-Hyun Kim ;Nelly S. Aggangan ;Pan-Gi Kim ;Kyung Joon Lee pp.602-608

보기
Ecological indicators of forest degradation after forest fire and clear-cutting in the Siberian larch (Larix sibirica) stand of Mongolia Yeong Dae Park ;Don Koo Lee ;John A. Stanturf ;Su Young Woo ;Damdinjav Zoyo pp.609-617

보기
Impact of transplanting on tree growth and compartmentalization of pruning wounds in Acer palmatum Thunb Kyu Hwa Lee ;Kyung Joon Lee ;Ki-Seob Gwak ;In-Gyu Choi pp.618-629

보기

참고문헌 (24건) : 자료제공( 네이버학술정보 )

참고문헌 목록에 대한 테이블로 번호, 참고문헌, 국회도서관 소장유무로 구성되어 있습니다.
번호 참고문헌 국회도서관 소장유무
1 Blears, M.J., Grandis. S.A., Lee, H. and Trevors, J.T.1998. Amplified fragment length polymorphism (AFLP): a review of the procedure and its applications. J. lnd.Microbiol. Biotechnol. 21: 99-114. 미소장
2 Organization of American and Asian Ginseng Germplasm Using Randomly Amplified Polymorphic DNA (RAPD) Markers 네이버 미소장
3 RFLP Maps Based on a Common Set of Clones Reveal Modes of Chromosomal Evolution in Potato and Tomato. 네이버 미소장
4 Development of Species Specific AFLP-Derived SCAR Marker for Authentication of Panax japonicus C. A. MEYER(Molecular and Cell Biology) 네이버 미소장
5 Genetic heterogeneity of ribosomal RNA gene and matK gene in Panax notoginseng. 네이버 미소장
6 Authentication of Panax ginseng and Panax quinquefolius using amplified fragment length polymorphism (AFLP) and directed amplification of minisatellite region DNA (DAMD). 네이버 미소장
7 Isolation and characterization of repetitive DNA sequences from Panax ginseng. 네이버 미소장
8 Plant Biotech 네이버 미소장
9 Kim, J., Jo, B.H., Lee, K.L., Yoon, E.S., Ryu, G.H. and Chung, K.W. 2007. Identification of new microsatellite markers in Panax ginseng. Mol. Cells 24: 60-68. 미소장
10 Kim, S.M. and Sohn, J.K. 2005. Identification of a rice gene (Bph 1) conferring resistance to brown planthopper (Nilaparvata lugens Stal) using STS marker. Mol. Cells 20: 30-34. 미소장
11 Kurata, N., Umehara, Y., Tanoue, H. and Sasaki, T. 1997. Physical mapping of the rice genome with YAC clones. Plant Mol. Biol. 35: 101-113. 미소장
12 Focus 네이버 미소장
13 Ma, K.H., Dixit, A., Kim, Y.C., Lee, D.Y., Kim, T.S., Cho, E.G. and Park, Y.J. 2007. Development and characterization of new microsatellite markers for ginseng (Panax ginseng C. A. Meyer). Conserv. Genet. 8: 1507- 1509. 미소장
14 Level of polymorphism and genetic mapping of AFLP markers in rice 네이버 미소장
15 Map-Based Cloning of a Protein Kinase Gene Conferring Disease Resistance in Tomato 네이버 미소장
16 DNA identification of commercial ginseng samples. 네이버 미소장
17 Molecular authentication of Panax species 네이버 미소장
18 Powell, W.W., Machery, G.C. and Provan, J. 1996. Polymorphism revealed by simple sequence repeats. Trends Genet. 1: 215-222. 미소장
19 Schluter, C. and Punja, Z.K. 2000, Genetic diversity among natural and cultivated populations and seed lots of American ginseng (Panax quinquefolius L.) in Canada. Int. J. Plant Sci. 163: 27-439. 미소장
20 Molecular Differentiation of Panax Species by RAPD Analysis 소장
21 Advanced backcross QTL analysis in a cross between an elite processing line of tomato and its wild relative L. pimpinellifolium. 네이버 미소장
22 Turnpenny, P. and Ellard, S. 2005. Emery's Elements of Medical Genetics, 12th. ed. Elsevier, London. 미소장
23 AFLP: a new technique for DNA fingerprinting. 네이버 미소장
24 Biotechnological studies in the Far-Eastern Region of Russia. 네이버 미소장

추천서가 (다양한 추천 자료를 만나보세요)

권호기사보기

권호기사 목록 테이블로 기사명, 저자명, 페이지, 원문, 기사목차 순으로 되어있습니다.
기사명 저자명 페이지 원문 기사목차

권호

기사명 원문보기 기사목차
닫기