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Background: Most lung cancer patients receive systemic chemotherapy at an advanced stage disease. Cisplatin-based chemotherapy is the main regimen for treating advanced lung cancer. Recently, autophagy has become an important mechanism of cellular adaptation under starvation or cell oxidative stress. The purpose of this study was to determine whether or not autophagy can occurred in cisplatin-treated lung cancer cells.

Methods: H460 cells were incubated with RPMI 1640 and treated in 5 μM or 20 μM cisplatin concentrations at specific time intervals. Cells surviving cisplatin treatment were measured and compared using an MTT cell viability assay to cells that underwent apoptosis with autophagy by nuclear staining, apoptotic or autophagic related proteins, and autophagic vacuoles. The development of acidic vascular organelles was using acridine orange staining and fluorescent expression of GFP-LC3 protein in its transfected cells was observed to evaluate autophagy.

Results: Lung cancer cells treated with 5 μM cisplatin-treated were less sensitive to cell death than 20 μM cisplatin-treated cells in a time-dependent manner. Nuclear fragmentation at 5 μM was not detected, even though it was discovered at 20 μM. Poly (ADP-ribose) polymerase cleavages were not detected in 5 μM within 24 hours. Massive vacuolization in the cytoplasm of 5 μM treated cells were observed. Acridine orange stain-positive cells was increased according in time-dependence manner. The autophagosome-incorporated LC3 II protein expression was increased in 5 μM treated cells, but was not detected in 20 μM treated cells. The expression of GFP-LC3 were increased in 5 μM treated cells in a time-dependent manner.

Conclusion: The induction of autophagy occurred in 5 μM dose of cisplatin-treated lung cancer cells.

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기사명 저자명 페이지 원문 목차
Prevention of lung cancer :future perspective with natural compounds Johann C. Brandes ;A.R.M. Ruhul Amin ;Fadlo Khuri ;Dong Moon Shin pp.1-15

폐암세포주에서 저용량 시스플라틴에 의해 유도된 자가포식 신정현 ;장혜연 ;정진수 ;조경화 ;황기은 ;김소영 ;김휘정 ;이삼윤 ;이미경 ; pp.16-23

신종 인플루엔자 A (H1N1) 감염으로 입원한 성인 폐렴 환자의 임상양상 한창훈 ;현여경 ;최유리 ;성나영 ;박윤선 ;이꽃실 ;정재호 pp.24-30

만성폐쇄성폐질환에 폐렴과 급성악화로 입원한 환자의 임상적 특성 및 예후 비교 정승욱 ;이재희 ;최금주 ;황보엽 ;김이영 ;이윤지 ;윤원경 ;김민 ;차승익 ;박 pp.31-38

(A)Case of Mycobacterium massiliense infection presenting as pneumonia resistant to antibiotics in an immunocompetent host Jung-Wan Yoo ;Yong Hee Kim ;Tae Sun Shim pp.39-42

고립성 폐결절로 발현된 비결핵성 마이코박테리움 폐질환 1예 김송이 ;이경종 ;이상훈 ;이상국 ;박병훈 ;정지예 ;손지영 ;윤여운 ;심효섭 ; pp.43-47

CEA상승을 보이는 폐 유육종증의 1예 양미연 ;유양선 ;고희자 ;박세경 ;박종숙 ;박춘식 ;박재성 ;백상현 ;고은석 ; pp.48-51

사지마비로 장기 침상안정 환자에서 폐결핵 발생부위 변화 1예 황헌규 ;정은정 ;임건일 ;양승부 ;임한혁 pp.52-55

참고문헌 (22건) : 자료제공( 네이버학술정보 )

참고문헌 목록에 대한 테이블로 번호, 참고문헌, 국회도서관 소장유무로 구성되어 있습니다.
번호 참고문헌 국회도서관 소장유무
1 Development by self-digestion: molecular mechanisms and biological functions of autophagy. 네이버 미소장
2 Autophagy as a cell death and tumor suppressor mechanism. 네이버 미소장
3 Reduced autophagic activity, improved protein balance and enhanced in vitro survival of hepatocytes isolated from carcinogen-treated rats 네이버 미소장
4 Tissue protein turnover during liver carcinogenesis 네이버 미소장
5 Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. 네이버 미소장
6 Characterization of the proteolytic compartment in rat hepatocyte nodules. 네이버 미소장
7 Overall proteolysis in perfused and subfractionated chemically induced malignant hepatoma of rat: effects of amino acids. 네이버 미소장
8 Cell biology: Autophagy and cancer 네이버 미소장
9 Bursch W, Ellinger A, Kienzl H, Török L, Pandey S,Sikorska M, et al. Active cell death induced by the anti-estrogens tamoxifen and ICI 164 384 in human mammary carcinoma cells (MCF-7) in culture: the role of autophagy. Carcinogenesis 1996;17:1595-607. 미소장
10 Induction of autophagic cell death in malignant glioma cells by arsenic trioxide. 네이버 미소장
11 A novel response of cancer cells to radiation involves autophagy and formation of acidic vesicles. 네이버 미소장
12 Molecular response of human glioblastoma multiforme cells to ionizing radiation: cell cycle arrest, modulation of the expression of cyclin-dependent kinase inhibitors, and autophagy. 네이버 미소장
13 Nonapoptotic Cell Death Associated with S-Phase Arrest of Prostate Cancer Cells via the Peroxisome Proliferator-activated Receptor gamma Ligand, 15-Deoxy-▵^1^2^,^1^4- prostaglandin J~2 네이버 미소장
14 Cisplatin and platinum analogues: recent advances. 네이버 미소장
15 Role of autophagy in breast cancer. 네이버 미소장
16 Unraveling the role of autophagy in cancer. 네이버 미소장
17 Anti- and pro-tumor functions of autophagy. 네이버 미소장
18 Levine B, Abrams J. p53: the Janus of autophagy? Nat Cell Biol 2008;10:637-9. 미소장
19 Autophagy: an emerging target for cancer therapy. 네이버 미소장
20 Role of Autophagy in Cancer: Management of Metabolic Stress 네이버 미소장
21 Control of autophagy by oncogenes and tumor suppressor genes. 네이버 미소장
22 Rubinsztein DC, Gestwicki JE, Murphy LO, Klionsky DJ.Potential therapeutic applications of autophagy. Nat Rev Drug Discov 2007;6:304-12. 미소장

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