대표어
한국학술지인용색인(NRF)
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초록보기
Accurate methods for the identification of closely related species or breeds in raw and processed meats must be developed
in order to protect both consumers and producers from mislabeling and fraud. This paper describes the development of DNA
markers for the discrimination and improvement of Korean native pig (KNP) meat. The KIT gene is related to pig coat color
and is often used as a candidate marker. A 538 bp fragment comprising intron 19 of the pig KIT gene was amplified by PCR
using specific primers, after which the PCR amplicons of a number of meat samples from KNP and three major improved
breeds (Landrace, Duroc and Yorkshire) were sequenced in order to find a nucleotide region suitable for PCR-RFLP analysis.
Sequence data showed the presence of two nucleotide substitutions, g.276G>A and g.295A>C, between KNP and the
improved pig breeds. Digestion of KIT amplicons with AccII enzyme generated characteristic PCR-RFLP profiles that
allowed discrimination between meats from KNP and improved pig. KNP showed three visible DNA bands of 264/249,
199, and 75 bp, whereas DNA bands of 249, 199, and 90 bp were detected in the three improved pig breeds. Therefore, the
75 bp DNA fragment was specific only to KNP, whereas the 90 bp DNA fragment was specific to the improved breeds. The
breed-specific DNA markers reported here that target the KIT gene could be useful for the identification of KNP meat from
improved pig meats, thus contributing to the prevention of falsified breed labeling.권호기사
참고문헌 (28건) : 자료제공( 네이버학술정보 )
| 번호 | 참고문헌 | 국회도서관 소장유무 |
|---|---|---|
| 1 | Technical note: A quick and more sensitive method to identify pork in processed and unprocessed food by PCR amplification of a new specific DNA fragment.  |
미소장 |
| 2 | Cho, S. H., Park, B. Y., Kim, J. H., Kim, M. J., Seong, P. N., Kim, Y. J., Kim, D. H., and Ahn, C. N. (2007) Carcass yields and meat quality by live weight of Korean native black pigs. Korean J. Anim. Aci. Technol. 49, 523-530. | 미소장 |
| 3 | Choi, Y. S., Park, B. Y., Lee, J. M., and Lee, S. K. (2005) Comparison of carcass and meat quality characteristics between Korean native black pigs and commercial crossbred pigs. Korean J. Food Sci. Ani. Resour. 25, 322-327 | 미소장 |
| 4 | Chung, E. R., Kim, W. T., Kim, Y. S., and Han, S. K. (2000) Identification of Hanwoo meat using PCR-RFLP marker of MC1R gene associated with bovine coat color. Korean J. Anim. Sci. Technol. 42. 379-380. | 미소장 |
| 5 | Colgan, S., O’Brien, L., Maher, M., Shilton, N., McDonnell, K., and Ward, S., (2001) Development of a DNA-based assay for species identification in meat and bone meal. Fleischwirtschaft 34, 409-414. | 미소장 |
| 6 | PCR identification of meats from chamois ( Rupicapra rupicapra), pyrenean ibex ( Capra pyrenaica), and mouflon ( Ovis ammon) targeting specific sequences from the mitochondrial D-loop region |
미소장 |
| 7 | Meat species identification by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of mitochondrial 12S rRNA gene |
미소장 |
| 8 | Giuffra, E., Evans G., Tornsten, A., Wales, R., Day A., Looft, H., Plastow, G., and Andersson, L. (1999) The belt mutation in pigs is an allele at the dominant white (I/KIT) locus. Mamm. Genome 10, 1132-1136. | 미소장 |
| 9 | Jin, S. K., Kim, C. W., Song, Y. M., Jang, W. H., Kim, Y. B., Yeo, J. S., Kim, J. W., and Kang, K. H. (2001) Physicochemical characteristics of longissimus muscle between the Korean native pig and Landrace. Korean J. Food Sci. Anim. Resour. 21, 142-148. | 미소장 |
| 10 | Johansson Moller, M., Chaudhary, R., Hellmen, E., Hoyheim, B., and Chowdhary, B., (1996) Pigs with the dominant white coat color phenotype carry a duplication of the KIT gene encoding the mast/stem cell growth factor receptor. Mamm. Genome 7, 822-830. | 미소장 |
| 11 | Kang, S. M., Kang, C. G., and Lee, S. K. (2007) Comparison of quality characteristics of korean native black pork and modern genotype pork during refrigerated storage after thawing. Korean J. Food. Sci. Ani. Resour. 27, 1-7. | 미소장 |
| 12 | PCR assay for the identification of animal species in cooked sausages |
미소장 |
| 13 | Melanocortin receptor 1 (MC1R) mutations and coat color in pigs. |
미소장 |
| 14 | Klungland, H., Vage, D. I., Gomez-Raya, L., Adalsteinsson, S., and Lien, S., (1995) The role of melanocyte-stimulating hormone (MSH) receptor in bovine coat colour determination. Mamm. Genome 6, 636-639. | 미소장 |
| 15 | Random amplified polymorphic DNA (RAPD) fingerprints for identification of red meat animal species |
미소장 |
| 16 | Lee, S. K., Ju, M. K., Kim, Y. S., Kang, S. M., and Choi, Y. S. (2005) Quality comparison between Korean native black groun pork and modern genotype pork during refrigerated storage. Kor J. Food Sci. Ani. Resour. 25, 71-77. | 미소장 |
| 17 | Determination of mitochondrial cytochrome B gene sequence for red deer ( Cervus elaphus) and the differentiation of closely related deer meats |
미소장 |
| 18 | A quick and simple method for the identification of meat species and meat products by PCR assay |
미소장 |
| 19 | Marklund, S., Moller, M., Sandberg, K., and Andersson, L. (1999) Close association between sequence polymorphism in the KIT gene and the roan coat color in horses. Mamm. Genome 10, 283-288. | 미소장 |
| 20 | Molecular basis for the dominant white phenotype in the domestic pig. |
미소장 |
| 21 | Mitochondrial markers for the detection of four duck species and the specific identification of Muscovy duck in meat mixtures using the polymerase chain reaction |
미소장 |
| 22 | Meyer, R., Hoefelein, C., Luethy, J., and Candrian, U. (1995) Polymerase chain reaction-restriction fragment length polymorphism analysis: a simple method for species identification in food. J. AOAC Intl. 78, 1542-1551. | 미소장 |
| 23 | Direct and highly species-specific detection of pork meat and fat in meat products by PCR amplification of mitochondrial DNA. |
미소장 |
| 24 | Okumura, N., Kobayashi, E., Suzuki, H., Morozumi, T., Hamashima, N., and Mitsuhashi, T. (2000) Breed specific mutations in MC1R and KIT genes in pigs. Anim. Sci. J. 8, 222-234. | 미소장 |
| 25 | Park, J. K., Shin, K. H., Shin, S. C., Chung, K. Y., and Chung, E. R. (2007) Identification of meat species using species- specific PCR-RFLP fingerprint of mitochondrial 12S rRNA gene. Korean J. Food Sci. Ani. Resour. 27, 209-215. | 미소장 |
| 26 | PCR-based fingerprinting techniques for rapid detection of animal species in meat products |
미소장 |
| 27 | Development of DNA markers for discrimination between domestic and imported beef |
미소장 |
| 28 | Shin, S. C., Chung, K. Y., and Chung, E. R. (2006) Identification of meat species using PCR-RFLP marker of cytochrome b gene. Korean J. Food. Sci. Ani. Resour. 26, 375- 379. | 미소장 |
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