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An experiment was conducted to examine the effects of Cordyceps militaris mycelia on microbial populations and fibrolytic enzyme activities in vitro. C. militaris mycelia was added to buffered rumen fluid with final concentrations of 0.00, 0.10, 0.15, 0.20, 0.25 and 0.30 g/L and incubation times were for 3, 6, 9, 12, 24, 36, 48 and 72 h. At all incubation times, the supplementation of C. militaris mycelia linearly increased the number of total viable and celluloytic bacteria; maximum responses were seen with 0.25 g/L supplementation of C. militaris mycelia. The addition of C. militaris mycelia above the level of 0.20 g/L significantly (p<0.01) increased the number of total and cellulolytic bacteria compared with the control. On the other hand, the response of fungal counts to the supplementation of C. militaris mycelia showed a linear decrease; the lowest response was seen with 0.30 g/L supplementation of C. militaris mycelia. It would seem that C. militaris mycelia possess a strong negative effect on rumen fungi since the lowest level of C. militaris mycelia supplementation markedly decreased fungal counts. Carboxylmethyl cellulase activities were linearly increased by the addition of C. militaris mycelia except at 3 and 9 h incubation times. At all incubation times, the supplementation of C. militaris mycelia linearly increased the activities of xylanase and avicelase. In conclusion, the supplementation of C. militaris mycelia to the culture of mixed rumen microorganisms showed a positive effect on cellulolytic bacteria and cellulolytic enzyme activities but a negative effect on fungi.

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참고문헌 (20건) : 자료제공( 네이버학술정보 )

참고문헌 목록에 대한 테이블로 번호, 참고문헌, 국회도서관 소장유무로 구성되어 있습니다.
번호 참고문헌 국회도서관 소장유무
1 Ahn, Y. J., S. J. Park, S. G. Lee, S. C. Shin and D. H. Choi. 2000. Cordycepin: selective growth inhibitor derived from liquid culture of Cordyceps militaris against Clostridium spp.. J. Agric. Food Chem. 48:2744-2748. 미소장
2 Inhibition of the cellulolytic activity of Neocallimastix frontalis by Ruminococcus flavefaciens 네이버 미소장
3 Dehority, B. A., P. A. Tirabasso and A. P. Grifo Jr. 1989. Most probable number procedures for enumerating ruminal bacteria, including the simultaneous estimation of total and cellulolytic numbers in one medium. Appl. Environ. Microbiol. 55:2789- 2792. 미소장
4 Holdman, L. V., E. P. Gato and W. E. C. Moore. 1977. Anaerobic laboratory manual, 4th ed. Virginia Polytechnic Institute and State University, Blacksburg, Virginia. 미소장
5 Hungate, R. E. 1966. The rumen bacteria. In: The rumen and its microbes (Ed. R. E. Hungate). Academic Press, New York and London, p. 8. 미소장
6 Kneifel, H., W. A. Konig, W. Loeffler and R. Muller. 1977. Ophiocordin, an antifungal antibiotic of Cordyceps ophioglossoides. Arch. Microbiol. 113:121-130. 미소장
7 Biotechnology: Promethean Science or Obsession? 네이버 미소장
8 BIOTECHNOLOGY IN AGRICULTURE: GOOD OR BAD? 네이버 미소장
9 Quality control of Cordyceps sinensis, a valued traditional Chinese medicine 네이버 미소장
10 Menke, K. H. and H. Steingass. 1988. Estimation of the energetic feed value obtained from chemical analysis and gas production using rumen fluid. Anim. Res. Dev. 28:7-55. 미소장
11 Measurement of carboxymethylcellulase activity 네이버 미소장
12 Inhibitory effect of Cordyceps sinensis on spontaneous liver metastasis of Lewis lung carcinoma and B16 melanoma cells in syngeneic mice. 네이버 미소장
13 Oripin, C. G. and K. N. Joblin. 1997. The rumen anaerobic fungi. In: The Rumen Microbial Ecosystem (Ed. P. N. Hobson and C. S. Stewart). Chapman and Hall, London, UK, pp. 140-195. 미소장
14 SAS Institute, 1996. SAS User’s Guide: Statistics. SAS Institute, Inc., Cary, NC, USA. 미소장
15 Williams, P. E. V., C. J. Newbold, A. Walker and R. J. Wallace. 1990. Rumen probiotics: The effects of including yeast culture (Saccharomyces ceresisiae plus growth medium) in the diet for sheep fed continuously or in meal fed steers. J. Dairy Sci. 72 (Suppl. 1):521 (Abstract). 미소장
16 Yeo, J. M., S. J. Lee, S. M. Lee, S. H. Shin, S. H. Lee, J. K. Ha, W. Y. Kim and S. S. Lee. 2009. Effects of Cordyceps militaris mycelia on in vitro rumen microbial fermentation. Asian-Aust. J. Anim. Sci. 22:201-205. 미소장
17 Yeon, S. H., J. R. Kim and Y. J. Ahn. 2007. Comparison of growth-inhibiting activities of Cordyceps militaris and Paecilomyces japonica cultured on Bombyx mori pupae towards human gastrointestinal bacteria. J. Sci. Food Agric. 87:54-59. 미소장
18 Pharmacological activities of stromata of Cordyceps scarabaecola 네이버 미소장
19 Isolation and biological properties of polysaccharide CPS-1 from cultured Cordyceps militaris 네이버 미소장
20 Isolation, purification and identification of polysaccharides from cultured Cordyceps militaris 네이버 미소장