본문 바로가기 주메뉴 바로가기
22대 대한민국 국회 국회정보길라잡이 국회의원검색
회원가입 로그인
HOME

정보검색

소장정보 검색

국회도서관 홈으로 정보검색 소장정보 검색

결과 내 검색

동의어 포함

고급검색

인명/단체명

저자정보 상세정보
인명/단체명을 입력하세요.

키워드

  • 대표어

  • 외국어

-

초록보기

Heat shock protein 90 (Hsp90) is ATPase-directed molecular chaperon and affects survival of cancer cell. Inhibitory effect of Hsp90 by inducing cell cycle arrest and apoptosis in the cancer cell was reported. However, its role during oocyte maturation and early embryo development is very insufficient. In this study, we traced the effects of Hsp90 inhibitor, 17-allylamino-17-demethoxygeldanamycin (17-AAG), on meiotic maturation and early embryonic development in pigs. We also investigated several indicators of developmental potential, including structural integrity, gene expression (Hsp90-, cell cycle-, and apoptosis-related genes), and apoptosis, which are affected by 17-AAG. Then, we examined the roles of Hsp90 inhibitor on viability of primary cells in pigs. Porcine oocytes were cultured in the NCSU-23 medium with or without 17-AAG for 44 h. The proportion of GV arrested oocytes was significantly different between the 17-AAG treated and untreated group (78.2 vs 34.8%, p<0.05). After completion of meiotic maturation, the proportion of MII oocytes was lower in the 17-AAG treated group than in the control group (27.9 vs 71.0%, p<0.05). After IVF, the percentage of penetrated oocytes was significantly lower in the 17-AAG treated group (25.2%), resulting in lower normal pronucleus formation (2PN of 14.6%). Therefore, the inhibition of meiotic progression by Hsp90 inhibitor played a critical role in fertilization status. Porcine embryo were cultured in the PZM-3 medium with or without 17-AAG for 6 days. In result, significant differences in developmental potential were detected between the embryos that were cultured with or without 17-AAG. Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) showed that the number of containing fragmented DNA at the blastocyst stage increased in the 17-AAG treated group compared with control (7.5 vs 4.4, respectively). Blastocysts that developed in the 17-AAG treated group had low structural integrity and high apoptotic nuclei than those of the untreated control, resulting in decrease the embryonic qualities of preimplantation porcine blastocysts. The mRNA expressions of cell cycle-related genes were down-regulated in the 17-AAG treated group compared with control. Also, the expression of the pro-apoptotic gene Bax increased in 17-AAG treated group, whereas expression of the anti-apoptotic gene Bcl-XL decreased. However, the expression of ER stress-related genes did not changed by 17-AAG. Cultured pESF cells were treated with or without 17-AAG and used for MTT assay. The results showed that viability of pESF cells were decreased by treatment of 17-AAG (2 μM) for 24 hr. These results indicated that 17-AAG decreased cell proliferation and increased cell death. Expression patterns Hsp90 complex genes (Hsp70 and p23), cell cycle-related genes (cdc2 and cdc25c) and apoptosis-related genes (Bax and Bcl-XL) were significantly changed by using RT-PCR analysis. The spliced form of pXbp-1 product (pXbp-1s) was detected in the tunicamycin (TM) treated cells, but it is not detected in 17-AAG treated cells. In conclusion, Hsp90 appears to play a direct role in porcine early embryo developmental competence including structural integrity of blastocysts. Also, these results indicate that Hsp90 is closely associated with cell cycle- and apoptosis-related genes expression in developing porcine embryos.

권호기사

권호기사 목록 테이블로 기사명, 저자명, 페이지, 원문, 기사목차 순으로 되어있습니다.
기사명 저자명 페이지 원문 목차
다중 역전사 중합효소 연쇄 반응(Multiplex RT-PCR)을 이용한 인간배아 줄기세포 및 유도만능 줄기세포의 효과적인 분화 양상 조사 김정모, 조윤정, 손온주, 홍기성, 정형민 pp.1-8

BMP4 처리에 의한 인간 배아줄기세포 유래 KDR 양성 중배엽성 세포군의 분화 양상 조사 김정모, 손온주, 조윤정, 이재호, 정형민 pp.9-15

Optimization of electrofusion condition for the production of Korean cattle somatic cell nuclear transfer embryos Sewoong Kim ,Daehwan Kim ,Yeon-Gil Jung ,Sangho Roh pp.17-22

Characterizations of cell lineage markers in the bone marrow cells of recloned GFP pigs for possible use of stem cell population Kwang-Wok Park, Sung Sik Choi, Dong Ho Lee, Hwang Lee, Seung Kyu Choi, Chang Sik Park, Sang Ho Lee pp.23-31

Hsp90 inhibitor induces cell cycle arrest and apoptosis of early embryos and primary cells in pigs Myeong-Ju Son ,Jin-Mo Park ,Sung-Hun Min ,Joo-Hee Hong ,Humdai Park ,Deog-Bon Koo pp.33-45

Estrogenic Xenobiotics가 돼지 정자의 운동성 및 운동역학에 미치는 영향 오신애, 박유진, 송원희, El-Sayed A. Mohamed, 방명걸 pp.47-54

고양이의 정액 채취 방법이 동결 정액의 생존성에 미치는 영향 하아나 ,윤진호 ,김유곤 ,조아라 ,이경림 ,공일근 pp.55-60

다양한 Percoll 세척 방법이 동결-융해된 한우 정자의 회수율 및 운동역학에 미치는 영향 윤성재 ,박유진 ,정진용 ,정규현 ,김민섭 ,유새미 ,김연희 ,권우성 ,El-Sayed A. Mohamed ,방명걸 pp.61-65

Methylation status of H19 gene in embryos produced by nuclear transfer of spermatogonial stem cells in pig Hyun Seung Lee, Sung Ho Lee, Mukesh Kumar Gupta, Sang Jun Uhm, Hoon Taek Lee pp.67-75

초자화 동결과 1-단계 융해된 체세포 핵이식란의 직접 이식 기술로 제주흑우 복제소 생산 김은영, 박민지, 김재연, 박효영, 노은지, 노은형, 송동환, 오창언, 김영훈, 문성호, 이동선, 고문석, 류기중, 박세필 pp.77-83

Percoll 분리된 미니돼지 정액의 체외 수정 능력에 있어서 동결보존액의 영향 이상희 ,유한준 ,이용승 ,정희태 ,양부근 ,김대영 ,박춘근 pp.85-91

돼지 체세포복제 배반포에서 Pre-1 영역의 Non-CpG 메틸화 양상 고응규 ,임기순 ,이휘철 ,조상래 ,최선호 ,최창용 ,이풍연 ,조창연 ,조재현 ,유용희 pp.93-97

돼지 난자의 체내 및 체외 성숙시 Transducin-like Enhancer Protein 1(TLE-1) mRNA의 발현 장예진 ,김동우 ,이용승 ,정희태 ,양부근 ,박춘근 pp.99-103

Nuclear modeling and developmental potential of bovine somatic nuclear transfer embryos cloned by two different activation methods Byeong-Gyun Jeon ,Gyu-Jin Rho pp.105-113

In Vitro에서 Retrovirus Vector System을 이용한 재조합 hFSH 유전자의 발현 민경헌, 권모선, 김태완, 구본철 pp.115-121

Establishment of porcine embryonic stem cells by aggregation of parthenogenetic embryos Jin Yu Zhang ,Yun Fei Diao ,Reza K. Oqani ,Je Sok Wo ,Dong Il Jin pp.123-129

참고문헌 (37건) : 자료제공( 네이버학술정보 )

참고문헌 목록에 대한 테이블로 번호, 참고문헌, 국회도서관 소장유무로 구성되어 있습니다.
번호 참고문헌 국회도서관 소장유무
1 Fertilization and subsequent development in vitro of pig oocytes inseminated in a modified tris-buffered medium with frozen-thawed ejaculated spermatozoa. 네이버 미소장
2 Ansamycin antibiotics inhibit Akt activation and cyclin D expression in breast cancer cells that overexpress HER2. 네이버 미소장
3 Genetic regulation of embryo death and senescence 네이버 미소장
4 Dimerization and N-Terminal Domain Proximity Underlie the Function of the Molecular Chaperone Heat Shock Protein 90 네이버 미소장
5 Functioning of the Hsp90 machine in chaperoning checkpoint kinase I (Chk1) and the progesterone receptor (PR). 네이버 미소장
6 Targeting the 90 kDa heat shock protein improves photodynamic therapy. 네이버 미소장
7 Combination Mammalian Target of Rapamycin Inhibitor Rapamycin and HSP90 Inhibitor 17-Allylamino-17-Demethoxygeldanamycin Has Synergistic Activity in Multiple Myeloma 네이버 미소장
8 In vitro development of in vitro-matured porcine oocytes following chemical activation or in vitro fertilization. 네이버 미소장
9 Inhibition of Hsp90 function by ansamycins causes downregulation of cdc2 and cdc25c and G(2)/M arrest in glioblastoma cell lines. 네이버 미소장
10 Georgakis GV, Li Y, Younes A (2006): The heat shock protein 90 inhibitor 17-AAG induces cell cycle arrest and apoptosis in mantle cell lymphoma cell lines by depleting cyclin D1, Akt, Bid and activating caspase 9. Br J Haematol 135:68-71. 미소장
11 Apoptosis in parthenogenetic preimplantation porcine embryos. 네이버 미소장
12 Tunicamycin sensitizes human melanoma cells to tumor necrosis factor-related apoptosis-inducing ligand-induced apoptosis by up-regulation of TRAIL-R2 via the unfolded protein response. 네이버 미소장
13 Binding of p23 and hsp90 during assembly with the progesterone receptor 네이버 미소장
14 Therapeutic and diagnostic implications of Hsp90 activation 네이버 미소장
15 17-Allylamino-17-demethoxygeldanamycin induces downregulation of critical Hsp90 protein clients and results in cell cycle arrest and apoptosis of human urinary bladder cancer cells. 네이버 미소장
16 Kaufman RJ (1999): Stress signaling from the lumen of the endoplasmic reticulum: coordination of gene transcriptional and translational controls. Genes Dev 13:1211-1233. 미소장
17 The assembly of progesterone receptor-hsp90 complexes using purified proteins. 네이버 미소장
18 Analysis of the roles of cyclin B1 and cyclin B2 in porcine oocyte maturation by inhibiting synthesis with antisense RNA injection. 네이버 미소장
19 Apoptosis in preimplantation mammalian embryo and genetics. 네이버 미소장
20 Li J, Ni M, Lee B, Barron E, Hinton DR, Lee AS (2008): The unfolded protein response regulator GRP78/BiP is required for endoplasmic reticulum integrity and stress-induced autophagy in mammalian cells. Cell Death Differ 15:1460-1471. 미소장
21 Development of early porcine embryos in vitro and in vivo. 네이버 미소장
22 Endoplasmic reticulum stress-induced death of mouse embryonic fibroblasts requires the intrinsic pathway of apoptosis. 네이버 미소장
23 The co-chaperone p23 arrests the Hsp90 ATPase cycle to trap client proteins. 네이버 미소장
24 Heat shock protein 90 네이버 미소장
25 Patterson J, Palombella VJ, Fritz C, Normant E (2008): IPI-504, a novel and soluble HSP-90 inhibitor, blocks the unfolded protein response in multiple myeloma cells. Cancer Chemother Pharmacol 61:923-932. 미소장
26 Culture of pig embryos. 네이버 미소장
27 Schumacher JA, Crockett DK, Elenitoba-Johnson KS, Lim MS (2007) Proteome-wide changes induced by the Hsp90 inhibitor, geldanamycin in anaplastic large cell lymphoma cells. Proteomics 7:2603-2616. 미소장
28 Hsp90 inhibitors cause G2/M arrest associated with the reduction of Cdc25C and Cdc2 in lung cancer cell lines. 네이버 미소장
29 Purification of unactivated progesterone receptor and identification of novel receptor-associated proteins. 네이버 미소장
30 17-Allylamino-17-demethoxygeldanamycin induces the degradation of androgen receptor and HER-2/neu and inhibits the growth of prostate cancer xenografts. 네이버 미소장
31 The influence of ATP and p23 on the conformation of hsp90. 네이버 미소장
32 Mediators of endoplasmic reticulum stress-induced apoptosis. 네이버 미소장
33 Taiyab A, Sreedhar AS, Rao CM (2009): Hsp90 inhibitors, GA and 17AAG, lead to ER stress-induced apoptosis in rat histiocytoma. Biochem Pharmacol 78: 142-152. 미소장
34 Heat shock protein 90 inhibition abrogates hepatocellular cancer growth through cdc2-mediated G2/M cell cycle arrest and apoptosis. 네이버 미소장
35 Hsp90 inhibitor 17-allylamino-17-demethoxygeldanamycin inhibits the proliferation of ARPE-19 cells. 네이버 미소장
36 XBP1 mRNA Is Induced by ATF6 and Spliced by IRE1 in Response to ER Stress to Produce a Highly Active Transcription Factor 네이버 미소장
37 Zhang DX, Cui XS, Kim NH (2010) Molecular characterization and polyadenylation-regulated expression of cyclin B1 and Cdc2 in porcine oocytes and early parthenotes. Mol Reprod Dev 77:38-50. 미소장

추천서가 (다양한 추천 자료를 만나보세요)

권호기사보기

권호기사 목록 테이블로 기사명, 저자명, 페이지, 원문, 기사목차 순으로 되어있습니다.
기사명 저자명 페이지 원문 기사목차

권호

기사명 원문보기 기사목차
닫기