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초록보기

The objective of this study was carried out to evaluate the efficiency of sperm collection methods on the post-thaw viability of cat semen. The cat semen was collected by artificial virginal (AV) and electronic ejaculate (EE) methods. The composition of semen extender was consisted of Tris-buffer supplemented with 20% egg yolk and 1% P/S antibiotics in Ext I, and more added 8% glycerol, 1.0% Equex STM paste of total volume in Ext II. The collected semen was adjusted the concentration and then diluted in Ext I for optimal concentration. The diluted semen was cooling to 5℃ temperature in refrigerator for at least 2 hrs and then diluted stepwise with Ext II for at least 1 hrs. After an equilibration for 1 hrs, the cooled semen was packaged in 0.5 ml straw and then freezing on the LN2 vapor over 5 cm above from LN2 and then immersed directly in LN2 for cryopreservation. The frozen semen was thawed in 38℃ water for 15 sec and then evaluated the motility, viability, and morphology. Post-thaw semen were calculated the motility by SMI (sperm motility index). The live-dead sperm was evaluated by Eosin-B and morphological evaluation was by Diff-quik kit staining. The post-thaw concentration (89×106 /ml vs. 128×106 /ml), viability (22.6±10.6% vs. 37.1±26.1%), morphological normality (27.0±50.2% vs. 45.6±123.0%) of EE and AV groups were not significant different, but the post-thaw motility was significant lower in EE than that in AV group (53.1±3.6 vs. 73.6±5.7) (p<0.05). In conclusion, semen collection methods did not significant different between EE and AV groups except of post-thaw motility and so both semen collection methods could be applied in feline semen collection methods.

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기사명 저자명 페이지 원문 목차
다중 역전사 중합효소 연쇄 반응(Multiplex RT-PCR)을 이용한 인간배아 줄기세포 및 유도만능 줄기세포의 효과적인 분화 양상 조사 김정모, 조윤정, 손온주, 홍기성, 정형민 pp.1-8

BMP4 처리에 의한 인간 배아줄기세포 유래 KDR 양성 중배엽성 세포군의 분화 양상 조사 김정모, 손온주, 조윤정, 이재호, 정형민 pp.9-15

Optimization of electrofusion condition for the production of Korean cattle somatic cell nuclear transfer embryos Sewoong Kim ,Daehwan Kim ,Yeon-Gil Jung ,Sangho Roh pp.17-22

Characterizations of cell lineage markers in the bone marrow cells of recloned GFP pigs for possible use of stem cell population Kwang-Wok Park, Sung Sik Choi, Dong Ho Lee, Hwang Lee, Seung Kyu Choi, Chang Sik Park, Sang Ho Lee pp.23-31

Hsp90 inhibitor induces cell cycle arrest and apoptosis of early embryos and primary cells in pigs Myeong-Ju Son ,Jin-Mo Park ,Sung-Hun Min ,Joo-Hee Hong ,Humdai Park ,Deog-Bon Koo pp.33-45

Estrogenic Xenobiotics가 돼지 정자의 운동성 및 운동역학에 미치는 영향 오신애, 박유진, 송원희, El-Sayed A. Mohamed, 방명걸 pp.47-54

고양이의 정액 채취 방법이 동결 정액의 생존성에 미치는 영향 하아나 ,윤진호 ,김유곤 ,조아라 ,이경림 ,공일근 pp.55-60

다양한 Percoll 세척 방법이 동결-융해된 한우 정자의 회수율 및 운동역학에 미치는 영향 윤성재 ,박유진 ,정진용 ,정규현 ,김민섭 ,유새미 ,김연희 ,권우성 ,El-Sayed A. Mohamed ,방명걸 pp.61-65

Methylation status of H19 gene in embryos produced by nuclear transfer of spermatogonial stem cells in pig Hyun Seung Lee, Sung Ho Lee, Mukesh Kumar Gupta, Sang Jun Uhm, Hoon Taek Lee pp.67-75

초자화 동결과 1-단계 융해된 체세포 핵이식란의 직접 이식 기술로 제주흑우 복제소 생산 김은영, 박민지, 김재연, 박효영, 노은지, 노은형, 송동환, 오창언, 김영훈, 문성호, 이동선, 고문석, 류기중, 박세필 pp.77-83

Percoll 분리된 미니돼지 정액의 체외 수정 능력에 있어서 동결보존액의 영향 이상희 ,유한준 ,이용승 ,정희태 ,양부근 ,김대영 ,박춘근 pp.85-91

돼지 체세포복제 배반포에서 Pre-1 영역의 Non-CpG 메틸화 양상 고응규 ,임기순 ,이휘철 ,조상래 ,최선호 ,최창용 ,이풍연 ,조창연 ,조재현 ,유용희 pp.93-97

돼지 난자의 체내 및 체외 성숙시 Transducin-like Enhancer Protein 1(TLE-1) mRNA의 발현 장예진 ,김동우 ,이용승 ,정희태 ,양부근 ,박춘근 pp.99-103

Nuclear modeling and developmental potential of bovine somatic nuclear transfer embryos cloned by two different activation methods Byeong-Gyun Jeon ,Gyu-Jin Rho pp.105-113

In Vitro에서 Retrovirus Vector System을 이용한 재조합 hFSH 유전자의 발현 민경헌, 권모선, 김태완, 구본철 pp.115-121

Establishment of porcine embryonic stem cells by aggregation of parthenogenetic embryos Jin Yu Zhang ,Yun Fei Diao ,Reza K. Oqani ,Je Sok Wo ,Dong Il Jin pp.123-129

참고문헌 (21건) : 자료제공( 네이버학술정보 )

참고문헌 목록에 대한 테이블로 번호, 참고문헌, 국회도서관 소장유무로 구성되어 있습니다.
번호 참고문헌 국회도서관 소장유무
1 Sperm morphology is better in the second ejaculate than in the first in domestic cats electroejaculated twice during the same period of anesthesia 네이버 미소장
2 Morphology of spermatozoa in the cauda epididymidis before and after electroejaculation and a comparison with ejaculated spermatozoa in the domestic cat 네이버 미소장
3 Sperm Morphology in the Domestic Cat, and its Relation with Fertility: A Retrospective Study 네이버 미소장
4 Bader H, Hoppen HO, Wokener A, Merkt H (1988): Case studies in stallions with fertility problems: Endocrine and spermatological aspects. Proc 11th Int Cong Anim Reprod Artif Insem (3):367. 미소장
5 Barth AD, Oko RJ (1989): Abnormal Morphology of Bovine Spermatozoa. Lowa State University Press Inc., Ames IA, USA. 미소장
6 Effects of Electroejaculation and Ketamine‐HCl on Serum Cortisol, Progesterone, and Testosterone in the Male Cat 네이버 미소장
7 Semen characteristics of genetically identical male cats cloned via somatic cell nucleus transfer 네이버 미소장
8 Effect of method of collection on seminal characteristics of the domestic cat. 네이버 미소장
9 Retrograde flow of spermatozoa into the urinary bladder of cats during electroejaculation, collection of semen with an artificial vagina, and mating. 네이버 미소장
10 Teratospermic and Normospermic Domestic Cats: Ejaculate Traits, Pituitary—Gonadal Hormones, and Improvement of Spermatozoal Motility and Morphology After Swim‐Up Processing 네이버 미소장
11 Zona pellucida filtration of structurally abnormal spermatozoa and reduced fertilization in teratospermic cats. 네이버 미소장
12 Semen Characteristics of the Captive Indian Leopard, Panthera pardus 네이버 미소장
13 Migaki G (1982): Compendium of inherited metabolic diseases in animals. Prog Clin Biol Res 94:473-501. 미소장
14 Comparison between different markers for sperm quality in the cat: Diff-Quik as a simple optical technique to assess changes in the DNA of feline epididymal sperm 네이버 미소장
15 Pregnancy in the domestic cat after artificial insemination with previously frozen spermatozoa. 네이버 미소장
16 Scott P, Cats in Hafez ES, editors (1970): Reproduction and Breeding Techniques for Laboratory Animals. Philadelphia: Lea and Febi-Ger 205. 미소장
17 Artificial insemination in the cat (Felis catus L.). 네이버 미소장
18 Reproductive physiology of the clouded leopard: I. Electroejaculates contain high proportions of pleiomorphic spermatozoa throughout the year. 네이버 미소장
19 Wildt DE, Phillips LG, Simmons LG, Chakraborty PK, Brown JL, Howard JG, Teare A, Bush MA (1988): Comparative analysis of ejaculate and hormonal characteristics of the captive male cheetah, tiger, leopard, and puma. BioI Reprod 38(2):245-255. 미소장
20 Semen collection in cats: Techniques and analysis 네이버 미소장
21 Effects of ketamine or medetomidine administration on quality of electroejaculated sperm and on sperm flow in the domestic cat 네이버 미소장

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