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A pentacyclic triterpenoid compound was isolated from the ethyl acetate extract of sedum (Sedum sarmentosum) and identified as D-friedoolean-13-en-3-one (taraxerone) by GC-MS and crystallographic analysis. The extraction yield of taraxerone was 74.12±0.57 mg/kg sedum (dry weight). The IC50 values of taraxerone were 102.34±1.53 μM and 1,763.81±12.63 μM/mL (Trolox equivalent) by the DPPH and ferric reducing ability of plasma (FRAP) assays, respectively. Taraxerone exhibited comparable antioxidant capacities with butylated hydroxytoluene (BHT) by the DPPH (p=0.117) and FRAP (p= 0.179) assays. The production of inducible nitric oxide in lipopolysaccharide-stimulated murine macrophage was inhibited by taraxerone (IC50=38.49±3.77 μM) via downregulation of inducible nitric oxide synthase (iNOS) expression at the transcriptional level. The inhibitory effect of taraxerone on nitric oxide generation was significantly more effective than that of caffeic acid and/or gallic acid.

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번호 참고문헌 국회도서관 소장유무
1 Botterweck AAM, Verhagen H, Goldbohm RA, Kleinjans J, Brandt PAVD. Intake of butylated hydroxyanisole and butylated hydroxytoluene and stomach cancer risk: Results from analyses in the Netherlands cohort study. Food Chem. Toxicol. 38: 599-605 (2000) 미소장
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3 Corlett JL, Clegg MS, Keen CL, Grivetti LE. Mineral content of culinary and medicinal plants by Hmong refugees living in Sacramento, California. Int. J. Food Sci. Nutr. 53: 117-128 (2002) 미소장
4 Mo EK, Kim HH, Kim SM, Cho HH, Sung CK. Production of sedum extract adding jelly and assessment of its physicochemical properties. Korean J. Food Sci. Technol. 39: 619-624 (2007) 미소장
5 Kim CY, Lee MY, Park I. Antioxidant activities of fractions from Sedum sarmentosum. J. Food Sci. Nutr. 11: 6-9 (2006) 미소장
6 Antioxidant activity and cytotoxicity of methanol extracts from aerial parts of Korean salad plants. 네이버 미소장
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10 Use of an aqueous soluble tetrazolium/formazan assay for cell growth assays in culture. 네이버 미소장
11 Dirsch VM, Stuppner H, Vollmar AM. The Griess assay: Suitable for a bio-guided fractionation of anti-inflammatory plant extracts? Planta Med. 64: 423-426 (1998) 미소장
12 Niki E. Assessment of antioxidant capacity in vitro and in vivo. Free Radical Bio. Med. 49: 503-513 (2010) 미소장
13 Updated methodology to determine antioxidant capacity in plant foods, oils and beverages: Extraction, measurement and expression of results 네이버 미소장
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16 Ahmed Y, Sohrab MH, Al-Reza SM, Tareq FS, Hasan CM, Sattar MA. Antimicrobial and cytotoxic constituents from leaves of Sapium baccatum. Food Chem. Toxicol. 48: 549-552 (2010) 미소장
17 Kuljanabhagavad T, Suttisri R, Pengsuparp T, Ruangrungsi N. Chemical structure and antiviral activity of aerial part from Laggera pterodonta. J. Health Res. 23: 175-177 (2009) 미소장
18 Antiparasitic compounds from Cupania cinerea with activities against Plasmodium falciparum and Trypanosoma brucei rhodesiense. 네이버 미소장
19 Wang XG. Isolation, structure identification, and insecticidal activity of compounds from Ficus tsiangii Merr. ex Corner. Acta Entomol. Sinica 53: 525-530 (2010) 미소장