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The molecular mechanisms underlying myogenic satellite cells (MSCs) differentiation into myotube-formed cells (MFCs) and transdifferentiation into adipocyte-like cells (ALCs) are unclear. As a step towards understanding the molecular mechanisms underlying MSC differentiation and transdifferentiation, we attempted to identify the genes differentially expressed during differentiation and transdifferentiation using gene microarray analysis (GMA). Thirty oligonucleotide arrays were used with two technical replicates and nine and six biological replicates for MFCs vs. MSCs and ALCs vs. MSCs, respectively, to contrast expression profile differences. GMA identified 1,224 differentially expressed genes by at least 2-fold during differentiation and transdifferentiation of MSCs. To select the highly expressed genes for future functional study, genes with a 4-fold expression difference were selected for validation by real time RT-PCR and approximately 96.9% of the genes were validated. The up-regulation of marker genes for myogenesis (MYL2, MYH3) and adipogenesis (PPARγ, and FABP4) was observed during the differentiation and transdifferentiation of MSCs into MFCs and ALCs, respectively. KOG analysis revealed that the most of the genes up-regulated during differentiation and transdifferentiation of MSCs were related to signal transduction. Again the exact location of 109 differentially expressed genes by 4-fold were analyzed by chromosome mapping. Among those, co-localization of 29 genes up-regulated during transdifferentiation with QTL for marbling score and intramuscular fat percentage supports the involvement of these genes in cellular transdifferentiation. Interestingly, some genes with unknown function were also identified during the process. Functional studies on these genes may unfold the molecular mechanisms controlling MSC differentiation and transdifferentiation.

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Expression analysis of lipopolysaccharide-and/or concanavalin A/phorbol myristate acetate-stimulated black rockfish peripheral blood leukocytes using a cDNA microarray to identify up-regulated genes Mun-Gyeong Kwon, Myoung-Ae Park, Jee-Youn Hwang, Gun-Wook Baeck, Jee-Min Jung, Ju-Won Kim, Dae-Won Park, Chan-Il Park pp.111-123

Gene expression profiles analyzed by DNA sequencing of cDNA clones constructed from porcine preadipocytes and adipocytes Dong-Mok Lee, Prati Bajracharya, Eun Jeong Jang, Eun Ju Lee, Sung Hwa Chae, Hong-Seog Park, Se Won Kang, Yong Seok Lee, Ki Ho Lee, Jongsoo Chang … [et al.] pp.125-131

Gene expression profiles during differentiation and transdifferentiation of bovine myogenic satellite cells Eun Ju Lee, Prati Bajracharya, Dong-Mok Lee, Se Won Kang, Yong Seok Lee, Hyun-Jeong Lee, Seong Koo Hong, Jongsoo Chang, Jae Woo Kim, Robert D. Schnabel … [et al.] pp.133-148

Phylogeny and divergence time estimation of Coreoleuciscus splendidus populations(Teleostei: Cypriniformes) endemic to Korea based on complete mitogenome sequences Keun-Yong Kim, Yoon Kwon Nam, In-Chul Bang pp.149-156

Analysis of genetic mapping in a waxy/dent maize RIL population using SSR and SNP markers Kyu Jin Sa, Jong Yeol Park, Kyong-Cheul Park, Ju Kyong Lee pp.157-164

LIPG promoter polymorphism is associated with ischemic stroke in Korean population No Soo Kim, Mi Mi Ko, Min Ho Cha, Ok-Sun Bang pp.165-171

The Drosophila melanogaster retinophilin gene encodes the peripheral membrane protein in photoreceptor cells Sunji Lee, Seong-Baek Lee, Paola Ramirez, Yuree Byun, Joosung Kim, Yongsu Jeong, Kwanghee Baek, Jaeseung Yoon pp.173-178

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In vitro CpG methylation and garcinol reduce PERV LTR promoter activity Hong-Seok Ha, Young-Choon Lee, Sang-Je Park, Yi-Deun Jung, Kung Ahn, Jae-Woo Moon, Kyudong Han, Keon-Bong Oh, Tae-Hun Kim, Hwan-Hoo Seong … [et al.] pp.217-222

In silico identification of potential drug targets in swine pathogen Haemophilus parasuis Zhuofei Xu, Lifang Ma, Huanchun Chen, Rui Zhou pp.223-230

Cholesterol ester transfer protein gene is associated with high-density lipoprotein cholesterol levels in Korean population Jae Woong Sull, Jong-Eun Lee, Myoungsook Lee, Sun Ha Jee pp.231-235

참고문헌 (28건) : 자료제공( 네이버학술정보 )

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번호 참고문헌 국회도서관 소장유무
1 Asakura A, Komaki M, and Rudnicki M (2001) Muscle satellite cellsare multipotential stem cells that exhibit myogenic, osteogenic,and adipogenic differentiation. Differentiation 68: 245-253. 미소장
2 Buckingham M, Bajard L, Chang T, Daubas P, Hadchouel J, MeilhacS, Montarras D, Hadchouel J, Meilhac S, and Montarras D (2003)The formation of skeletal muscle: from s&omite to limb. J. Anat.202: 59-68. 미소장
3 A novel function for fatty acid translocase (FAT)/CD36: involvement in long chain fatty acid transfer into the mitochondria. 네이버 미소장
4 Casas E, Shackelford SD, Keele JW, Koohmaraie M, Smith TPL,and Stone RT (2003) Detection of quantitative trait loci for growthand carcass composition in cattle. J. Anim. Sci. 81: 2976-2983. 미소장
5 Coburn CT, Knapp Jr. FF, Febbraio M, Beets AL, Silverstein RL,and Abumrad NA (2000) Defective uptake and utilization oflong-chain fatty acids in muscle and adipose tissues of CD36knockout mice. J. Biol. Chem. 275: 32523-32529. 미소장
6 CD36 mediates both cellular uptake of very long chain fatty acids and their intestinal absorption in mice. 네이버 미소장
7 Dual‐regulated expression of C/EBP‐α and BMP‐2 enables differential differentiation of C2C12 cells into adipocytes and osteoblasts 네이버 미소장
8 Development and Application of Bovine and Porcine Oligonucleotide Arrays with Protein-Based Annotation 네이버 미소장
9 Hawke TJ, and Garry DJ (2001) Myogenic satellite cells: physiologyto molecular biology. J. Appl. Physiol. 91: 534-551. 미소장
10 Hu E, Tontonoz P, and Spiegelman BM (1995) Transdifferentiationof myoblasts by the adipogenic transcription factors PPAR gammaand C/EBPα. Proc. Natl. Acad. Sci. USA 92: 9856-9860. 미소장
11 Kook SH, Choi KC, Son YO, Lee KY, Hwang IH, Lee HJ, ChangJS, Choi IH, and Lee JC (2006) Satellite cells isolated from adultHanwoo muscle can proliferate and differentiate into myoblastsand adipose-like cells. Mol. Cells 22: 239-245. 미소장
12 Lee DM, Bajracharya P, Lee EJ, Lee HJ, Kim JE, Lee HJ, ChunT, Kim J, Cho K H, Chang J et al. (2011). In Vitro Cell. Develop.Biol. - Animal 47: 438-444. 미소장
13 Mauro A (1961) Satellite cells of skeletal muscle fibres. J. Biophys.Biochem. Cytol. 9: 493-495. 미소장
14 A genome scan for quantitative trait loci influencing carcass, post‐natal growth and reproductive traits in commercial Angus cattle 네이버 미소장
15 Endocrinology Reviewers, 2007 네이버 미소장
16 Moran JL, Li Y, Hill AA, Mounts WM, and Miller CP (2002) Geneexpression changes during mouse skeletal myoblast differentiationrevealed by transcriptional profiling. Physiol. Genomics 10: 103-111. 미소장
17 Poulos SP, and Hausman GJ A (2006) comparison of thiazolidinedione-induced adipogenesis and myogenesis in stromal-vascularcells from subcutaneous adipose tissue or semitendinosus muscleof postnatal pigs. J. Anim. Sci. 84: 1076-1082. 미소장
18 Cell 네이버 미소장
19 Shen X, Collier JM, Hlaing M, Zhang L, Delshad EH, Bristow J,and Bernstein HS (2003) Genome-wide examination of myoblastcell cycle withdrawal during differentiation. Dev. Dyn. 226: 128-138. 미소장
20 Singh NK, Chae HS, Hwang IH, Yoo YM, Ahn CN, Lee SH, LeeHJ, Park HJ, and Chung HY (2007) Transdifferentiation of porcinesatellite cells to adipoblasts with ciglitizone. J. Anim. Sci.85: 1126-1135. 미소장
21 Sterrenburg E, Turk R, ’ Hoen PAC, van Deutekom JCT, Boer JM,van Ommen GJ, and den Dunnen JT (2004) Large-scale geneexpression analysis of human skeletal myoblast differentiation.Neuromuscul. Disord. 14: 507-518. 미소장
22 Tajbakhsh S, Rocancourt D, and Buckingham M (1996) Muscle progenitorcells failing to respond to positional cues adopts non-myogenicfates in myf-5 null mice. Nature 384: 266-270. 미소장
23 Tan SH, Reverter A, Wang YH, Byrne KA, McWilliam SM, andLehnert SA (2006) Gene expression profiling of bovine in vitroadipogenesis using a cDNA microarray. Funct Integr Genomics.6: 235-249. 미소장
24 Candidate gene analysis of GH1 for effects on growth and carcass composition of cattle. 네이버 미소장
25 Thiazolidinediones and fatty acids convert myogenic cells into adipose-like cells. 네이버 미소장
26 Tomczak KK, Marinescu VD, Tamoni MF, Sanoudou D, MontanaroF, Han M, Kunkel LM, Kohane IS, and Beggs AH (2003)Expression profiling and identification of novel genes involvedin myogenic differentiation. The FASEB J. 18: 403-405. 미소장
27 Wheeler TL, Cundiff LV, and Koch RM (1994) Effect of marblingdegree on beef palatability in Bos taurus and Bos indicus cattle.J. Anim. Sci. 72: 3145-3151. 미소장
28 Zhou J, Febbraio M, Zhai Y, Kuruba R, Wada T, Khadem S, RenS, Li S, Silverstein RL, and Xie W (2008) LXR, PXR, and PPARγcooperate in regulating fatty acid transporter CD36 and promotinghepatic lipogenesis. Gastroenterology 134: 556-567. 미소장

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