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This study aims to investigate the photoprotective properties of a Lomentaria hakodatensis ethanol extract (LHE) against ultraviolet B (UVB) radiationinduced cellular damage in human HaCaT keratinocytes.

LHE exhibited scavenging activity against intracellular reactive oxygen species (ROS), which were generated by either hydrogen peroxide (H2O2) or UVB radiation.

Moreover, LHE scavenged superoxide anion generated by the xanthine/xanthine oxidase system and hydroxyl radical generated by the Fenton reaction (FeSO4 + H2O2).

Furthermore, LHE exhibited UVB absorptive properties and attenuated injury to cellular components (e.g., lipids,proteins and DNA), resulting from UVB-induced oxidative stress. In addition, LHE reduced apoptosis in response to UVB, as shown by decreased DNA fragmentation and the formation of apoptotic bodies. These results suggest that LHE protects human keratinocytes against UVB-induced oxidative stress by scavenging ROS and absorbing UVB rays; thereby reducing damage to biological components.

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참고문헌 (19건) : 자료제공( 네이버학술정보 )

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2 Ramachandran, S., N. Rajendra Prasad, and S. Karthikeyan (2010) Sesamol inhibits UVB-induced ROS generation and subsequent oxidative damage in cultured human skin dermal fibroblasts. Arch. Dermatol. Res. 302: 733-744. 미소장
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6 Maeda, H., T. Tsukui, T. Sashima, M. Hosokawa, and K. Miyashita (2008) Seaweed carotenoid, fucoxanthin, as a multi-functional nutrient. Asia. Pac. J. Clin. Nutr. 1: 196-199. 미소장
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10 Lee, O. H., K. Y. Yoon, K. J. Kim, S. G. You, and B. Y. Lee (2011) Seaweed extracts as a potential tool for the attenuation of oxidative damage in obesity-related pathologies. J. Phycol. 47: 548-556. 미소장
11 Kricker, A., B. K. Armstrong, C. Goumas, M. Litchfield, C. B. Begg, A. J. Hummer, L. D. Marrett, B. Theis, R. C. Millikanm, N. Thomas, H. A. Culver, R. P. Gallagher, T. Dwyer, T. R. Rebbeck, P. A. Kanetsky, K. Busam, L. From, U. Mujumdar, R. Zanetti, and M. Berwick (2007) Ambient UV, personal sun exposure and risk of multiple primary melanomas. Cancer Causes & Control 18: 295-304. 미소장
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13 Shick, J. M. and W. C. Dunlap (2002) Mycosporine-like amino acids and related Gadusols: Biosynthesis, acumulation, and UVprotective functions in aquatic organisms. Annu. Rev. Physiol. 64: 223-262. 미소장
14 de la Coba, F., J. Aguilera, M. V. de Gálvez, M. Alvarez, E. Gallego, F. L. Figueroa, and E. Herrera (2009) Prevention of the ultraviolet effects on clinical and histopathological changes, as well as the heat shock protein-70 expression in mouse skin by topical application of algal UV-absorbing compounds. J. Dermatol. Sci. 55: 161-169. 미소장
15 Torres, A., C. D. Enk, M. Hochberg, and M. Srebnik (2006) Porphyra- 334, a potential natural source for UVA protective sunscreens. Photochem. Photobiol. Sci. 5: 432-435. 미소장
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17 Xia, Q., J. J. Yin, W. G. Wamer, S. H. Cherng, M. D. Boudreau, P. C. Howard, H. Yu, and P. P. Fu (2006) Photoirradiation of retinyl palmitate in ethanol with ultraviolet light--formation of photodecomposition products, reactive oxygen species, and lipid peroxides. Int. J. Environ. Res. Public Health 3: 185-190. 미소장
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19 Vostalova, J., A. Zdarilova, and A. Svobodova (2010) Prunella vulgaris extract and rosmarinic acid prevent UVB-induced DNA damage and oxidative stress in HaCaT keratinocytes. Arch. Dermatol. Res. 302: 171-181. 미소장