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초록보기
Rotaviruses have been known to be a major etiological agent of gastroenteritis in both infants and young animals. Subsequently new rotaviruses, which were morphologically indistinguishable but antigenically and electrophoretically distinct with each other, were reported from several animals throughout world including Korea. These new rotaviruses were named as non-group A or group B or group C rotaviruses and so on. It has been very difficult to isolate and grow the non-group A rotaviruses in vitro, and this has greatly limited the characterizations of non-group A rotaviruses and serological studies. In this study, monoclonal antibodies (MAbs) against porcine non-group A rotavirus were produced and characterized. The VP6 gene of porcine group C rotavirus Korean isolate(#06-52-1) was cloned and expressed. For expression of VP6 gene, baculovirus expression system was applied. The VP6 gene and expressed protein in the recombinant virus were confirmed by polymerase chain reaction (PCR), indirect fluorescence antibody (IFA) test and Western blot, respectively. The expressed VP6 was used for MAbs production. The MAbs produced in this study would be promising as diagnostic reagents for detection of group C rotavirus infection.
권호기사
참고문헌 (23건) : 자료제공( 네이버학술정보 )
| 번호 | 참고문헌 | 국회도서관 소장유무 |
|---|---|---|
| 1 | Rotavirus infection in adults  |
미소장 |
| 2 | Bridger JC. 1987. Novel rotaviruses in animals and man. Ciba Found Symp 128: 5-23. | 미소장 |
| 3 | Detection and characterization of group C rotaviruses in asymptomatic piglets in Ireland. |
미소장 |
| 4 | Rotavirus gene structure and function. |
미소장 |
| 5 | Synthesis and immunogenicity of the rotavirus major capsid antigen using a baculovirus expression system. |
미소장 |
| 6 | Detection and molecular characterization of porcine group C rotaviruses in South Korea |
미소장 |
| 7 | Biochemical characterization of the structural and nonstructural polypeptides of a porcine group C rotavirus. |
미소장 |
| 8 | Reactivity of VP4-specific monoclonal antibodies to a serotype 4 porcine rotavirus with distinct serotypes of human (symptomatic and asymptomatic) and animal rotaviruses. |
미소장 |
| 9 | Characterization of group C rotaviruses associated with diarrhea outbreaks in feeder pigs. |
미소장 |
| 10 | Biochemical and immunologic comparison of virus-like particles for a rotavirus subunit vaccine |
미소장 |
| 11 | Ojeh CK, Jiang BM, Tsunemitsu H, Kang SY, Weilnau PA, Saif LJ. 1991. Reactivity of monoclonal antibodies to the 41-kilodalton protein of porcine group C rotavirus with homologous and heterologous rotavirus serogroups in immunofluorescence tests. J Clin Microbiol 29: 2051- 2055. | 미소장 |
| 12 | Modifications of hybridoma technology which improve the yield of monoclonal antibody producing cells |
미소장 |
| 13 | Global illness and deaths caused by rotavirus disease in children. |
미소장 |
| 14 | Genetically Diverse Group C Rotaviruses Cause Sporadic Infection in Korean Calves |
미소장 |
| 15 | Peralta A, Molinari P, Taboga O. 2009. Chimeric recombinant rotavirus- like particles as a vehicle for the display of heterologous epitopes. J Virol 6: 192-201. | 미소장 |
| 16 | Detection of a rotavirus-like agent associated with diarrhea in an infant. |
미소장 |
| 17 | Serial propagation of porcine group C rotavirus (pararotavirus) in a continuous cell line and characterization of the passaged virus. |
미소장 |
| 18 | Comparison of atypical rotaviruses from calves, piglets, lambs and man. |
미소장 |
| 19 | Thouless ME, Beards GM, Flewett TH. 1982. Serotyping and subgrouping of rotavirus strains by the ELISA test. Arch Virol 73: 219-230. | 미소장 |
| 20 | Expression of the major capsid protein VP6 of group C rotavirus and synthesis of chimeric single-shelled particles by using recombinant baculoviruses. |
미소장 |
| 21 | Electrophoretic Transfer of Proteins from Polyacrylamide Gels to Nitrocellulose Sheets: Procedure and Some Applications |
미소장 |
| 22 | Immunogenicity of baculovirus expressed recombinant proteins of Japanese encephalitis virus in mice | 소장 |
| 23 | Enzyme-Linked Immunosorbent Assay for Identification of Rotaviruses from Different Animal Species |
미소장 |
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