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This study examined the motility of either the unattached(upper) or attached(lower) Hanwoo sperm to bovine oviduct epithelial cell(BOEC) monolayers to determine whether there are any changes in their motility during co-culture.

The cleavage and blastocyst development rate were compared among different preincubation methods in-vitro, after oocytes were fertilized in-vitro with Hanwoo sperm on BOEC monolayers. The motility of frozen-thawed sperm in BOEC co-culture group was significantly higher than controls, especially at 5 hours and 6 hours (p<0.05) of incubation, in sperm treatment medium without heparin and caffeine. The motility of frozen-thawed sperm in BOEC co-culture group was significantly higher than controls, especially at 3 hours (p<0.05) and 6 hours (p<0.01), in sperm treatment medium containing heparin and caffeine. The motility of the attached( lower) sperm was significantly higher than the unattached(upper) sperm during co-culture with BOEC at all times(p<0.01 or p<0.05), except for 6 hours.

After Hanwoo oocytes were fertilized in-vitro with the sperm that had been co-cultured with BOEC in sperm treatment medium containing heparin and caffeine, we determined the cleavage and blastocyst development rate, according to the preincubation methods. Both the cleavage and blastocyst development rate from 2 hour preincubation group were the highest, but significant difference was not recognized. These results show that BOEC plays an important role on sperm hyperactivation related to capacitation regardless of heparin and caffeine in sperm treatment medium. However, oviduct epithelial cell had no significant effect on the development of embryos after in-vitro fertilization in the presence of added heparin and caffeine in sperm treatment medium.

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참고문헌 (25건) : 자료제공( 네이버학술정보 )

참고문헌 목록에 대한 테이블로 번호, 참고문헌, 국회도서관 소장유무로 구성되어 있습니다.
번호 참고문헌 국회도서관 소장유무
1 Different signaling pathways in bovine sperm regulate capacitation and hyperactivation. 네이버 미소장
2 Capacitation of rabbit spermatozoa in vitro. 네이버 미소장
3 Fertilizing ability of bovine spermatozoa cocultured with oviduct epithelial cells. 네이버 미소장
4 Roles of the oviduct in mammalian fertilization. 네이버 미소장
5 Hyperactivated sperm progress in the mouse oviduct. 네이버 미소장
6 Carbohydrates mediate the adherence of hamster sperm to oviductal epithelium. 네이버 미소장
7 Role of carbohydrates in the attachment of equine spermatozoa to uterine tubal (oviductal) epithelial cells in vitro. 네이버 미소장
8 Spermadhesin AQN1 is a candidate receptor molecule involved in the formation of the oviductal sperm reservoir in the pig. 네이버 미소장
9 Behavior of bull spermatozoa in bovine uterine tube epithelial cell co-culture: An in vitro model for studying the cell interactions of reproduction 네이버 미소장
10 Selection of highly fertilization-competent bovine spermatozoa through adhesion to the Fallopian tube epithelium in vitro. 네이버 미소장
11 PDC-109 (BSP-A1/A2) promotes bull sperm binding to oviductal epithelium in vitro and may be involved in forming the oviductal sperm reservoir. 네이버 미소장
12 Hwang WS, Roh SH and Lee BC. 1998. Fertilizing ability of bovine spermatozoa following oviduct epithelial cell coculture in vitro. J. Embryo Transf. 13: 227-233. 미소장
13 Ignotz G, Lo M, Perez C, Gwathmey T and Suarez S. 2001. Characterization of a fucose-binding protein from bull sperm and seminal plasma that may be responsible for formation of the oviductal sperm reservoir. Biol. Reprod. 64:1806- 1811. 미소장
14 Iqbal N and Hunter AG. 1995. Effect of various capacitation systems on bovine sperm motion characteristics, acrosome integrity, and induction of hyperactivation. J. Dairy Sci. 78 :91-102. 미소장
15 Effect of capacitation on bull sperm binding to homologous oviductal epithelium. 네이버 미소장
16 Hyperactivation may assist human spermatozoa to detach from intimate association with the endosalpinx 네이버 미소장
17 Differences in the role of cyclic adenosine 3',5'-monophosphate during capacitation of bovine sperm by heparin or oviduct fluid. 네이버 미소장
18 Fertilizing Capacity of Bovine Sperm may be Maintained by Binding to Oviductal Epithelial Cells1 네이버 미소장
19 Roh SH, Lee BC and Hwang WS. 1999. The ability of in vitro cultured bovine oviduct epithelial cells in binding and maintaining motility of bull sperm. Korean J. Vet. Res. 39: 220- 225. 미소장
20 Relationship between bull field fertility and in vitro embryo production using sperm preparation methods with and without somatic cell co-culture 네이버 미소장
21 Attachment and release of spermatozoa from the caudal isthmus of the hamster oviduct. 네이버 미소장
22 Sperm binding properties and secretory activity of the bovine oviduct immediately before and after ovulation 네이버 미소장
23 Sperm motility hyperactivation facilitates penetration of the hamster zona pellucida. 네이버 미소장
24 Sperm transport and motility in the mouse oviduct: observations in situ. 네이버 미소장
25 Res 네이버 미소장

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