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A screening for cellobiohydrolase (CBH) activity was

performed and Fomitopsis pinicola KMJ812 was selected

for further characterization as it produced a high level of

CBH activity. An extracellular CBH was purified to

homogeneity by sequential chromatography of F. pinicola

culture supernatants. The molecular mass of the F. pinicola

CBH was determined to be 64 kDa by SDS-PAGE and by

size-exclusion chromatography, indicating that the enzyme

is a monomer. The F. pinicola CBH showed a t1/2 value of

42 h at 70oC and catalytic efficiency of 15.8 mM?1 s?1 (kcat/

Km) for p-nitrophenyl-β-D-cellobioside, one of the highest

levels seen for CBH-producing microorganisms. Its internal

amino acid sequences showed a significant homology with

hydrolases from glycoside hydrolase family 7. Although

CBHs have been purified and characterized from other

sources, the F. pinicola CBH is distinguished from other

CBHs by its high catalytic efficiency and thermostability.

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권호기사 목록 테이블로 기사명, 저자명, 페이지, 원문, 기사목차 순으로 되어있습니다.
기사명 저자명 페이지 원문 목차
Purification and Characterization of a Thermostable Cellobiohydrolase from Fomitopsis pinicola 신게움, 김윤희, Marimuthu Jeya, 이정걸, 김영숙 pp.1681-1688

A Genetic Comparison of Brucella abortus Isolates from Animals and Humans by Using an MLVA Assay 허문, 강성일, 김종완, 김지연, 황인영, 정숙찬, 박상희, 박미연, 유한상 pp.1750-1755

Assessment of Root-Associated Paenibacillus polymyxa Groups on Growth Promotion and Induced Systemic Resistance in Pepper Phi, Quyet-Tien, Yu-Mi Park, Keyung-Jo Seul, Choong-Min Ryu, Seung-Hwan Park, Jong-Guk Kim, Sa-Youl Ghim pp.1605-1613

Antigenicity of Partial Fragments of Recombinant Pasteurella multocida Toxin 이정민, 우희종 pp.1756-1763

Cloning and Expression of a Thermostable α-Galactosidase from the Thermophilic Fungus Talaromyces emersonii in the Methylotrophic Yeast Pichia pastoris Similä, Janika, Anita Gernig, Patrick Murray, Maria G. Tuohy, Sara Fernandes pp.1653-1663

Bacterial Community Composition and Diversity of a Full-Scale Integrated Fixed-Film Activated Sludge System as Investigated by Pyrosequencing 권순동, 김택성, 유기현, 정준홍, 박희등 pp.1717-1723

Comparison of the Genomes of Deinococcal Species Using Oligonucleotide Microarrays 정선욱, 조민호, 임성훈, 김동호, 임상용 pp.1637-1646

Repeated Random Mutagenesis of α-Amylase from Bacillus licheniformis for Improved pH Performance Priyadharshini, Ramachandran, Shankar Manoharan, Devaraj Hemalatha, Paramasamy Gunasekaran pp.1696-1701

Cold-Adapted and Rhizosphere-Competent Strain of Rahnella sp. with Broad-Spectrum Plant Growth-Promotion Potential Vyas, Pratibha, Robin Joshi, K. C. Sharma, Praveen Rahi, Ashu Gulati, Arvind Gulati pp.1724-1734

Gibberellin-Producing Endophytic Fungi Isolated from Monochoria vaginalis Ahmad, Nadeem, Abdul Latif Khan, 이인정, 신동현, Sumera Afzal Khan, Muhammad Hamayun pp.1744-1749

Identification of Streptomyces sp. KH29, Which Produces an Antibiotic Substance Processing an Inhibitory Activity Against Multidrug-Resistant Acinetobacter baumannii 이경호, 이기형, 김계웅 pp.1672-1676

Purification and Characterization of a Ubiquitin-like System for Autophagosome Formation 배주영, 박현호 pp.1647-1652

Identification and Characterization of a Pantothenate Kinase(PanK-sp) from Streptomyces peucetius ATCC 27952 Mandakh, Ariungerel, Narayan Prasad Niraula, 김응필, 송재경 pp.1689-1695

Selection of Peptides Binding to HCV E2 and Inhibiting Viral Infectivity 홍혜원, 이성욱, 명희준 pp.1769-1771

Characterization of Yakju Brewed from Glutinous Rice and Wild-Type Yeast Strains Isolated from Nuruks 김혜련, 김재호, 배동훈, 안병학 pp.1702-1710

Mutations in the gyrB, parC, and parE Genes of Quinolone-Resistant Isolates and Mutants of Edwardsiella tarda 김명숙, Hyun Do Jeong, 전류진, 박명애, 정성희, Kwangil Kim, 신순범, Kyung Ho Moon pp.1735-1743

Repressed Quorum Sensing by Overexpressing LsrR Hampers Salmonella Evasion from Oxidative Killing Within Macrophages 최정준, 박주원, 유상렬 pp.1624-1629

Genetic and Phenotypic Diversity of Plant Growth Promoting Rhizobacteria Isolated from Sugarcane Plants Growing in Pakistan Mehnaz, Samina, Deeba N. Baig, George Lazarovits pp.1614-1623

Response of Saccharomyces cerevisiae to Ethanol Stress Involves Actions of Protein Asr1p Ding, Junmei, Xiaowei Huang, Na Zhao, Feng Gao, Ke-Qin Zhang, Qian Lu pp.1630-1636

Antibiotic Resistance Profiles of Staphylococcus pseudintermedius Isolates from Canine Patients in Korea 윤장원, 이기종, 이소영, 박재건, 박희명, 유종현, 채민주 pp.1764-1768

Characterization of a Paenibacillus woosongensis β-Xylosidase/α-Arabinofuranosidase Produced by Recombinant Escherichia coli 김연아, 윤기홍 pp.1711-1716

Bioproduction and Anticancer Activity of Biosurfactant Produced by the Dematiaceous Fungus Exophiala dermatitidis SK80 Chiewpattanakul, Paramaporn, Sirinet Phonnok, Alain Durand, Emmanuelle Marie, Benjamas Wongsatayanon Thanomsub pp.1664-1671

The Effect of Protectants and pH Changes on the Cellular Growth and Succinic Acid Yield of Mannheimia succiniciproducens LPK7 오영훈, 정창규, 오인재, 이상엽, 이진원 pp.1677-1680

참고문헌 (38건) : 자료제공( 네이버학술정보 )

참고문헌 목록에 대한 테이블로 번호, 참고문헌, 국회도서관 소장유무로 구성되어 있습니다.
번호 참고문헌 국회도서관 소장유무
1 Baldeian, P. and V. Valaskova. 2008. Degradation of cellulose by basidiomycetous fungi. FEMS Microbiol. Rev. 32: 501-521. 미소장
2 Degradation of cellulose and hemicelluloses by the brown rot fungus Piptoporus betulinus--production of extracellular enzymes and characterization of the major cellulases. 네이버 미소장
3 Cellulose, cellulases and cellulosomes. 네이버 미소장
4 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding 네이버 미소장
5 Kjeldahl Method for Organic Nitrogen 네이버 미소장
6 Bukhtojarov, F. E., B. B. Ustinov, T. N. Salanovich, A. L. Antonov, A. V. Gusakov, O. N. Okunev, and A. P. Sinitsyn. 2004. Cellulose complex of the fungus Chrysosporium lucknowense: Isolation and characterization of endogluconases and cellobiohydrolases. Biochemistry 69: 542-551. 미소장
7 Purification and some properties of �-glucosidase from the ectomycorrhizal fungus Pisolithus tinctorius strain SMF 네이버 미소장
8 An assay for selective determination of exo-1,4,-β-glucanases in a mixture of cellulolytic enzymes 네이버 미소장
9 The Three-Dimensional Crystal Structure of the Catalytic Core of Cellobiohydrolase I from Trichoderma reesei 네이버 미소장
10 Isolation of fungal cellobiohydrolase I genes from sporocarps and forest soils by PCR. 네이버 미소장
11 Three-dimensional structure of a thermostable native cellobiohydrolase, CBH IB, and molecular characterization of the cel7 gene from the filamentous fungus, Talaromyces emersonii. 네이버 미소장
12 Biotechnological studies in the Far-Eastern Region of Russia. 네이버 미소장
13 Gusakov, A. V., A. P. Sinitsyn, T. N. Salanovich, F. E. Bukhtojarov, A. V. Markov, B. B. Ustinov, C. van Zeijl, P. Punt, and R. Burlingame. 2005. Purification, cloning and characterization of two forms of thermostable and highly active cellobiohydrolase I (Cel7A) produced by the industrial strain of Chrysosporium lucknowense. Enz. Microbial Technol. 36: 57-69. 미소장
14 Haakana, H., A. Miettinen-Oinonen, V. Joutsjoki, A. Mantyla, P. Suominen, and J. Vehmaanpera. 2004. Cloning of cellulose genes from Melanocarpus albomyces and their efficient expression in Trichoderma reesei. Enz. Microbial Technol. 34: 159-167. 미소장
15 Purification, Characterization and Gene Analysis of Exo-Cellulase II(Ex-2)from the White Rot Basidiomycete Irpex lacteus 네이버 미소장
16 A classification of glycosyl hydrolases based on amino acid sequence similarities. 네이버 미소장
17 Biotechnology: Promethean Science or Obsession? 네이버 미소장
18 Cloning of the cbhI and cbhII genes involved in cellulose utilisation by the straw mushroom Volvariella volvacea. 네이버 미소장
19 Biodegradative mechanism of the brown rot basidiomycete Gloeophyllum trabeum: evidence for an extracellular hydroquinone-driven fenton reaction 네이버 미소장
20 Cloning, sequencing, and heterologous expression of a cellulase-encoding cDNA (cbhl) from Penicillium janthinellum 네이버 미소장
21 Konstantinidis, A. K., I. Marsden, and M. L. Sinnott. 1993. Hydrolyses of alpha- and beta-cellobiosyl fluorides by cellobiohydrolases of Trichoderma reesei. J. Biochem. 291: 883-888. 미소장
22 Cleavage of structural proteins during the assembly of the head of bacteriophage T4. 네이버 미소장
23 Biotechnological studies in the Far-Eastern Region of Russia. 네이버 미소장
24 Cloning and characterization of two cellulase genes from Lentinula edodes 네이버 미소장
25 Liete, R. S. R., E. Gomes, and R. Da-Silva. 2007. Characterization of β-glucosidases from a mesophilic Aureobasidium pullulana and thermophilic Thermoascus aurantiacus. Process Biochem. 42: 1101-1106. 미소장
26 Limam, F., S. E. Chaabouni, R. Ghrir, and N. Marzouki. 1995. Two cellobiohydrolases of Penicillium occitanis mutant Pol 6: Purification and properties. Enz. Microbial Technol. 17: 340-346. 미소장
27 Purification and biochemical characteristics of �-D-glucosidase from a thermophilic fungus, Thermomyces lanuginosus. SSBP 네이버 미소장
28 Li, Y. L., D. C. Li, and F. C. Teng. 2006. Purification and characterization of a cellobiohydrolase from the thermophilic fungus Chaetomium thermophilus CT2. Wei Sheng Wu Xue Bao 46: 143-146. 미소장
29 Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar 네이버 미소장
30 Muñoz, I. G., W. Ubhayaseker, H. Henriksson, I. Szabó, G. Pettersson, G. Johansson, S. L. Mowbray, and J. Ståhlberg. 2001. Family 7 cellobiohydrolases from Phanerochaete chrysosporium: Crystal structure of the catalytic module of Cel7D (CBH58) at 1.32 A resolution and homology models of the isozymes. J. Mol. Biol. 314: 1097-1111. 미소장
31 Thermostability and irreversible activity loss of exoglucanase/xylanase Cex from Cellulomonas fimi. 네이버 미소장
32 Crystal structures of Melanocarpus albomyces cellobiohydrolase Cel7B in complex with cello‐oligomers show high flexibility in the substrate binding 네이버 미소장
33 Biotechnology and bioeconomy in China. 네이버 미소장
34 Purification and properties of two enzymes from Dichomitus squalens which exhibit both cellobiohydrolase and xylanase activity 네이버 미소장
35 Purification and Characterization of Two Exo-cellobiohydrolases from the Brown-Rot Fungus Coniophora puteana (Schum ex Fr) Karst. 네이버 미소장
36 Crystalline cellulose degradation: new insight into the function of cellobiohydrolases 네이버 미소장
37 Homologous domains in Trichoderma reesei cellulolytic enzymes: Gene sequence and expression of cellobiohydrolase II 네이버 미소장
38 Kinetic parameters and mode of action of the cellobiohydrolases produced by Talaromyces emersonii 네이버 미소장

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