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Because conventional methods for detecting emetic-toxinproducing

B. cereus are laborious and costly, various PCR

assays, which are easy and cheap, have recently been

reported. Therefore, this study estimated and compared

the ability of various PCR assays to detect emetic-toxinproducing

B. cereus strains isolated in Korea. The PCR

assays were performed on 160 B. cereus strains, including

40 emetic-toxin-producing strains. Although the speciesspecific

PCR assays were all shown to be highly specific,

the sensitivities varied greatly. The accuracies of the

primers were 97.5% (CER), 95.6% (EM1), 96.3% (RE234),

89.4% (CES), and 83.1% (Ces3R/CESR2). Moreover, the

CER primer had a higher sensitivity (100%) than all the

other primers tested, and a specificity of 96.7%. Thus, the

CER primer was shown to be the most effective for

screening the emetic-toxin-producing B. cereus strains

tested in this study. However, the ability of these PCR

assays to identify emetic-toxin-producing B. cereus should

also be confirmed using other methods.

권호기사

권호기사 목록 테이블로 기사명, 저자명, 페이지, 원문, 기사목차 순으로 되어있습니다.
기사명 저자명 페이지 원문 목차
Improvement in Sensory Characteristics of Campbell Early Wine by Adding Dual Starters of Saccharomyces cerevisiae and Oenococcus oeni 유기선, 한남수, 김지은, 서은영, 최화영, 윤향식, 김명동, 김유진 pp.1121-1127

Isolation, Characterization, and Application of Chitosan-Degrading Fungus from Soil Wei, Xinlin, Wei Chen, Ming Xiao, Jianbo Xiao, Yuanfeng Wang pp.1114-1120

irrE, an Exogenous Gene from Deinococcus radiodurans, Improves the Growth of and Ethanol Production by a Zymomonas mobilis Strain Under Ethanol and Acid Stresses Zhang, Ying, Zhonglin Zhao, Zhengfu Zhou, Wei Zhang, Ming Chen, Wei Lu, Ruiqiang Ma pp.1156-1162

Purification and Characterization of a Laccase from the Edible Wild Mushroom Tricholoma mongolicum Li, Miao, Guoqing Zhang, Hexiang Wang, Tzibun Ng pp.1069-1076

Study of Macrophage Activation and Structural Characteristics of Purified Polysaccharide from the Fruiting Body of Cordyceps militaris 이종석, 권정석, 원동필, 이중현, 이신영, 홍억기, 이권억 pp.1053-1060

Establishment of an Axenic Culture of Microcystin-Producing Microcystis aeruginosa Isolated from a Korean Reservoir 한아원, 오경희, 정원화, 조영철 pp.1152-1155

Chronic Toxicity of the Triazole Fungicide Tebuconazole on a Heterocystous, Nitrogen-Fixing Rice Paddy Field Cyanobacterium, Westiellopsis prolifica Janet Nirmal Kumar, J. I., Anubhuti Bora, Manmeet Kaur Amb pp.1134-1139

Evaluation of Various PCR Assays for Detection of Emetic-Toxin-Producing Bacillus cereus 김정범, 김재명, 박용배, 한정아, 이순호, 곽효순, 황인균, 윤미혜, 이종복, 오덕환 pp.1107-1113

Gellan Gum as Immobilization Matrix for Production of Cyclosporin A Survase, Shrikant A., Uday S. Annapure, Rekha S. Singhal pp.1086-1091

Biochemical and Thermal Stabilization Parameters of Polygalacturonase from Erwinia carotovora subsp. carotovora BR1 Maisuria, V. B., V. A. Patel, A. S. Nerurkar pp.1077-1085

Characterization of Bacterial Structures in a Two-Stage Moving-Bed Biofilm Reactor(MBBR) During Nitrification of the Landfill Leachate Ciesielski, Slawomir, Dorota Kulikowska, Ewelina Kaczowka, Przemysław Kowal pp.1140-1151

Can a Fermentation Gas Mainly Produced by Rumen Isotrichidae Ciliates be a Potential Source of Biohydrogen and a Fuel for a Chemical Fuel Cell? Piela, Piotr, Tadeusz Michałowski, Renata Miltko, Krzysztof W. Szewczyk, Radosław Sikora, Elżbieta Grzesiuk, Anna Sikora pp.1092-1100

Synthesis of Biosurfactant-Based Silver Nanoparticles with Purified Rhamnolipids Isolated from Pseudomonas aeruginosa BS-161R Kumar, C. Ganesh, Suman Kumar Mamidyala, G. Sarala Devi, Biswanath Das, B. Sridhar, Mallampalli SriLakshmi Karuna pp.1061-1068

Accumulation of Selenium and Changes in the Activity of Inulinase and Catalase in the Cells of Kluyveromyces marxianus on Pulsed Electric Field Treatment Pankiewicz, Urszula, Jerzy Jamroz pp.1101-1106

Diversity and Abundance of Ammonia-Oxidizing Bacteria in Activated Sludge Treating Different Types of Wastewater 백경화, 오희목, 김희식, 박철, 윤병대 pp.1128-1133

참고문헌 (30건) : 자료제공( 네이버학술정보 )

참고문헌 목록에 대한 테이블로 번호, 참고문헌, 국회도서관 소장유무로 구성되어 있습니다.
번호 참고문헌 국회도서관 소장유무
1 A novel dodecadepsipeptide, cereulide, isolated fromBacillus cereus causes vacuole formation in HEp-2 cells 네이버 미소장
2 A novel sensitive bioassay for detection of Bacillus cereus emetic toxin and related depsipeptide ionophores. 네이버 미소장
3 Arnesen, L. P. S., A. Fagerlund, and P. E. Granum. 2008. Bacillus cereus and its food poisoning toxins. FEMS Microbiol. Rev. 32: 579-606. 미소장
4 Bartoszewicz, M., B. M. Hansen, and I. Swiecicka. 2008. The members of the Bacillus cereus group are commonly present contaminants of fresh and heat-treated milk. Food Microbiol. 25: 588-596. 미소장
5 A comparison of ELISA and RPLA for detection of Bacillus cereus diarrhoeal enterotoxin. 네이버 미소장
6 Production and characterization of antibodies against each of the three subunits of the Bacillus cereus nonhemolytic enterotoxin complex. 네이버 미소장
7 Duc, L. H., T. C. Ding, N. A. Logan, A. D. Sutherland, J. Taylor, and S. M. Cutting. 2005. Cases of emesis associated with bacterial contamination of an infant breakfast cereal product. Int. J. Food Microbiol. 102: 245-251. 미소장
8 Ehling-Schulz, M., M. Fricker, and S. Scherer. 2004. Identification of emetic toxin producing Bacillus cereus strains by a novel molecular assay. FEMS Microbiol. Lett. 232: 189-195. 미소장
9 Identification and partial characterization of the nonribosomal peptide synthetase gene responsible for cereulide production in emetic Bacillus cereus. 네이버 미소장
10 Granum, P. E. 1990. Clostridium perfringens toxins involved in food poisoning. Int. J. Food Microbiol. 10: 101-111. 미소장
11 How to read a paper: Papers that report diagnostic or screening tests 네이버 미소장
12 Quantitative analysis of cereulide, the emetic toxin of Bacillus cereus, produced under various conditions. 네이버 미소장
13 Evidence for non-ribosomal peptide synthetase production of cereulide (the emetic toxin) in Bacillus cereus. 네이버 미소장
14 Jääskeläinen, E. L., V. Teplova, M. A. Adersson, L. C. Andersson, P. Tammela, M. C. Andersson, et al. 2003. In vitro assay for human toxicity of cereulide, the emetic mitochondrial toxin produced by food poisoning Bacillus cereus. Toxicol. In Vitro 17: 737-744. 미소장
15 Quantitative Analysis of Cereulide, an Emetic Toxin of Bacillus cereus, by Using Rat Liver Mitochondria 네이버 미소장
16 Kim, J. H., E. G. Lim, H. C. Jang, J. Y. Park, S. J. Lee, M. S. Park, G. B. Chli, and B. K. Lee. 2009. A case of emetic toxin producing Bacillus cereus strains isolated from outbreak. Korean J. Clin. Microbiol. 12: 48-52. 미소장
17 Koneman, E., S. D. Allen, W. M. Janda, P. C. Schreckenberger, and W. C. Winn Jr. 1997. The aerobic Gram-positive bacilli, pp. 651-708. In: Color Atlas and Textbook of Diagnostic Microbiology, 5th Ed. Lippincott, New York. 미소장
18 Kramer, J. M. and R. J. Gilbert. 1992. Bacillus cereus gastroenteritis, pp. 119-153. In A. T. Tu (ed.). Food Poisoning - Handbook of Natural Toxins, Vol. 7. Marcel Dekker, Inc., New York. 미소장
19 Kramer, J. M. and R. J. Gilbert. 1989. Bacillus cereus and other Bacillus species, pp. 21-70. In M. P. Doyle (ed.). Foodborne Bacterial Pathogens. Marcel Dekker, New York. 미소장
20 A new cytotoxin from Bacillus cereus that may cause necrotic enteritis. 네이버 미소장
21 McKillip, J. L. 2000. Prevalence and expression of enterotoxins in Bacillus cereus and other Bacillus spp. Antonie van Leeuwenhoek 77: 393-399. 미소장
22 Mikkola, R., N.-E. L. Saris, P. A. Grigoriev, M. A. Andersson, and M. S. Salkinoja-Salonen. 1999. Ionphoretic properties and mitochondrial effect of cereulide. Eur. J. Biochem. 262: 112-117. 미소장
23 A PCR assay based on a sequence-characterized amplified region marker for detection of emetic Bacillus cereus. 네이버 미소장
24 The hemolytic enterotoxin HBL is broadly distributed among species of the Bacillus cereus group. 네이버 미소장
25 Bacillus cereus food poisoning and its toxins. 네이버 미소장
26 Toxin Gene Profiling of Bacillus cereus Food Isolates by PCR 소장
27 Microarray analysis of Bacillus cereus group virulence factors 네이버 미소장
28 Mouse Lethal Activity of a HEp-2 Vacuolation Factor, Cereulide, Produced by Bacillus cereus Isolated from Vomiting-Type Food Poisoning 네이버 미소장
29 Toh, M., M. C. Moffitt, L. Henrichsen, M. Raftery, K. Barrow, J. M. Cox, C. P. Marquis, and B. A. Neilan. 2004. Cereulide, the emetic toxin of Bacillus cereus, is putatively a product of nonribosomal peptide synthesis. J. Appl. Microbiol. 97: 992- 1000. 미소장
30 Exploring the Domain Structure of Modular Nonribosomal Peptide Synthetases 네이버 미소장

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