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A highly efficient cellobiohydrolase (CBH)-secreting

basidiomycetous fungus, Agaricus arvensis KMJ623, was

isolated and identified based on its morphological features

and sequence analysis of internal transcribed spacer

rDNA. An extracellular CBH was purified to homogeneity

from A. arvencis culture supernatant using sequential

chromatography. The relative molecular mass of A.

arvencis CBH was determined to be 65 kDa by SDSPAGE

and 130 kDa by size-exclusion chromatography,

indicating that the enzyme is a dimer. A. arvencis CBH

showed a catalytic efficiency (k_(cat)/K_m) of 31.8 mM^(-1) s^(-1) for

p-nitrophenyl-β-D-cellobioside, the highest level seen for

CBH-producing microorganisms. Its internal amino acid

sequences showed significant homology with CBHs from

glycoside hydrolase family 7. Although CBHs have been

purified and characterized from other sources, A. arvencis

CBH is distinguished from other CBHs by its high catalytic

efficiency.

권호기사

권호기사 목록 테이블로 기사명, 저자명, 페이지, 원문, 기사목차 순으로 되어있습니다.
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Statistical Optimization of Medium Composition for Bacterial Cellulose Production by Gluconacetobacter hansenii UAC09 Using Coffee Cherry Husk Extract : an Agro-Industry Waste Rani, Mahadevaswamy Usha, Navin K. Rastogi, K. A. Anu Appaiah pp.739-745

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참고문헌 (32건) : 자료제공( 네이버학술정보 )

참고문헌 목록에 대한 테이블로 번호, 참고문헌, 국회도서관 소장유무로 구성되어 있습니다.
번호 참고문헌 국회도서관 소장유무
1 Fatty acid and sugar compositions, and nutritional value of five wild edible mushrooms from Northeast Portugal 네이버 미소장
2 Cellulose, cellulases and cellulosomes. 네이버 미소장
3 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding 네이버 미소장
4 Bukhtojarov, F. E., B. B. Ustinov, T. N. Salanovich, A. I. Antonov, A. V. Gusakov, O. N. Okunev, and A. P. Sinitsyn. 2004. Cellulase complex of the fungus Chrysosporium lucknowense: Isolation and characterization of endoglucanases and cellobiohydrolases. Biochemistry 69: 542-551. 미소장
5 An assay for selective determination of exo-1,4,-β-glucanases in a mixture of cellulolytic enzymes 네이버 미소장
6 Doran-Peterson, J., A. Jangid, S. K. Brandon, E. DeCrescenzo- Henriksen, B. Dien, and L. O. Ingram. 2009. Simultaneous saccharification and fermentation and partial saccharification and co-fermentation of lignocellulosic biomass for ethanol production. Methods Mol. Biol. 581: 263-280. 미소장
7 Edwards, I. P., R. A. Upchurch, and R. Z. Donald. 2008. A classification of glycosyl hydrolases based on amino acid sequence similarity. Appl. Environ. Microbiol. 74. 74: 3481- 3489. 미소장
8 Gusakov, A. V., A. P. Sinitsyn, T. N. Salanovich, F. E. Bukhtojarov, A. V. Markov, B. B. Ustinov, C. Van Zeijl, P. Punt, and R. Burlingame. 2005. Purification, cloning and characterization of two forms of thermostable and highly active cellobiohydrolase I (Cel7A) produced by the industrial strain of Chrysosporium lucknowense. Enzyme Microb. Technol. 36: 57-69. 미소장
9 Haakana, H., A. Miettinen-Oinonen, V. Joutsjoki, A. Mantyla, P. Suominen, and J. Vehmaanpera. 2004. Cloning of cellulose genes from Melanocarpus albomyces and their efficient expression in Trichoderma reesei. Enzyme Microb. Technol. 34: 159-167. 미소장
10 Purification, Characterization and Gene Analysis of Exo-Cellulase II(Ex-2)from the White Rot Basidiomycete Irpex lacteus 네이버 미소장
11 A classification of glycosyl hydrolases based on amino acid sequence similarities. 네이버 미소장
12 Biotechnology: Promethean Science or Obsession? 네이버 미소장
13 Cloning of the cbhI and cbhII genes involved in cellulose utilisation by the straw mushroom Volvariella volvacea. 네이버 미소장
14 The genes encoding glycoside hydrolase family 6 and 7 cellulases from the brown-rot fungus Coniophora puteana 네이버 미소장
15 Cloning, sequencing, and heterologous expression of a cellulase-encoding cDNA (cbhl) from Penicillium janthinellum 네이버 미소장
16 Cleavage of structural proteins during the assembly of the head of bacteriophage T4. 네이버 미소장
17 Biotechnological studies in the Far-Eastern Region of Russia. 네이버 미소장
18 Cloning and characterization of two cellulase genes from Lentinula edodes 네이버 미소장
19 Li, Y. L., D. C. Li, and F. C. Teng. 2006. Purification and characterization of a cellobiohydrolase from the thermophilic fungus Chaetomium thermophilus CT2. Wei Sheng Wu Xue Bao 46: 143-146. 미소장
20 Liete, R. S. R., E. Gomes, and R. Da-Silva. 2007. Characterization of β-glucosidases from a mesophilic Aureobasidium pullulana and thermophilic Thermoascus aurantiacus. Process Biochem. 42: 1101-1106. 미소장
21 Limam, F., S. E. Chaabouni, R. Ghrir, and N. Marzouki. 1995. Two cellobiohydrolases of Penicillium occitanis mutant Pol 6: Purification and properties. Enzyme Microb. Technol. 17: 340- 346. 미소장
22 Purification and biochemical characteristics of �-D-glucosidase from a thermophilic fungus, Thermomyces lanuginosus. SSBP 네이버 미소장
23 Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar 네이버 미소장
24 Biotechnological studies in the Far-Eastern Region of Russia. 네이버 미소장
25 Biotechnology and bioeconomy in China. 네이버 미소장
26 A ‘capillary racetrack’ method for isolation of magnetotactic bacteria 네이버 미소장
27 Purification and properties of two enzymes from Dichomitus squalens which exhibit both cellobiohydrolase and xylanase activity 네이버 미소장
28 Purification and Characterization of Two Exo-cellobiohydrolases from the Brown-Rot Fungus Coniophora puteana (Schum ex Fr) Karst. 네이버 미소장
29 Crystalline cellulose degradation: new insight into the function of cellobiohydrolases 네이버 미소장
30 Homologous domains in Trichoderma reesei cellulolytic enzymes: Gene sequence and expression of cellobiohydrolase II 네이버 미소장
31 Kinetic parameters and mode of action of the cellobiohydrolases produced by Talaromyces emersonii 네이버 미소장
32 White, T. J., T. Bruns, S. Lee, and J. Taylor. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics, pp. 315-322. In M. A. Innis, D. H. Gelfand, J. J. Sninsky, and T. J. White (eds.). PCR Protocols: A Guide to Methods and Applications Academic Press, Inc., New York, 미소장