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In this study, the antibacterial and antioxidative activities of Epimedium koreanum Nakai were investigated for applications as cosmetic ingredients. Minimum inhibitory concentrations (MICs) of fraction-bacterium, that showed high antibacterial activity from disc diffusion assay on human skin pathogens, were tested. The ethyl acetate fraction on Staphylococcus aureus, Bacillus subtilis, Propionibacterium acnes and 50% ethanol extract on S. aureus exhibited higher antibacterial activities than methyl paraben, well known as a preservative. The DPPH (1,1-diphenyl-2-picrylhydrazyl) scavenging activities of 3 fractions of E. koreanum Nakai were lower than (+)-α-tocopherol, known as a typical antioxidant. From the results of the scavenging activities of various ROS generated in Fe3+-EDTA/H2O2 systems (OSC50), 50% ethanol extract (OSC50 = 2.46 ± 0.06 μg/ml) and aglycone fraction (OSC50 = 1.45 ± 0.02 μg/ml) showed high activities similar to L-ascorbic acid (OSC50 = 1.50 ± 0.85 μg/ml), used as reference. The cellular protective effects (τ50) on photohemolysis by 1O2 generated by photosensitization reaction were tested. The cellular protective effect of 50% ethanol extract (τ50 = 37.0 ± 0.3 min) was similar to (+)-α-tocopherol (τ50 = 38.0 ± 1.8 min), used as reference. In particular, the τ50 of aglycone fraction results were 165.9 ± 7.2 min. This is a high cellular protective effect, more than 4 times that of (+)-α-tocopherol. These results indicate that E. koreanum Nakai extract, and its fractions, could be utilized as a cosmetic ingredient possessing antibacterial and antioxidative activities.
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참고문헌 (24건) : 자료제공( 네이버학술정보 )
| 번호 | 참고문헌 | 국회도서관 소장유무 |
|---|---|---|
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| 2 | Labisia pumila extract protects skin cells from photoaging caused by UVB irradiation(CELL AND TISSUE ENGINEERING)  |
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미소장 |
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| 8 | The roles of cytokines in photoaging. |
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| 19 | Seo MS, Kim KY. 2010. Effects of lithospermum erythrorhizon extracts on P. acnes induced cytokin gene expression in human monocytes. Korean J. Oriental Medical Ophthalmology & Otolaryngology & Dermatology Society 23: 57-68. | 미소장 |
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