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Metallic and non-metallic isomerases can be used to produce

commercially important monosaccharides. To determine

which category of isomerase is more suitable as a template

for directed evolution to improve enzymes for galactose

isomerization, L-arabinose isomerase from Escherichia coli

(ECAI; E.C. 5.3.1.4) and tagatose-6-phosphate isomerase

from Staphylococcus aureus (SATI; E.C. 5.3.1.26) were

chosen as models of a metallic and non-metallic isomerase,

respectively. Random mutations were introduced into the

genes encoding ECAI and SATI at the same rate, resulting

in the generation of 515 mutants of each isomerase. The

isomerization activity of each of the mutants toward a

non-natural substrate (galactose) was then measured. With

an average mutation rate of 0.2 mutations/kb, 47.5% of

the mutated ECAIs showed an increase in activity compared

with wild-type ECAI, and the remaining 52.5% showed a

decrease in activity. Among the mutated SATIs, 58.6%

showed an increase in activity, whereas 41.4% showed a

decrease in activity. Mutant clones showing a significant

change in relative activity were sequenced and specific

increases in activity were measured. The maximum increase

in activity achieved by mutation of ECAI was 130%, and

that for SATI was 190%. Based on these results, the

characteristics of the different isomerases are discussed in

terms of their usefulness for directed evolution of nonnatural

substrate isomerization.

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권호기사 목록 테이블로 기사명, 저자명, 페이지, 원문, 기사목차 순으로 되어있습니다.
기사명 저자명 페이지 원문 목차
Comparative Proteomic Analysis of Human Amniotic Fluid Supernatants with Down Syndrome Using Mass Spectrometry 박지숙, 차동현, 정진우, 김영환, 이숙환, 김영준, 김광표 pp.959-967

Improvement of Transformation Efficiency Through In Vitro Methylation and SacII Site Mutation of Plasmid Vector in Bifidobacterium longum MG1 김진용, Yan Wang, 박명수, 지근억 pp.1022-1026

Monitoring of Possible Horizontal Gene Transfer from Transgenic Potatoes to Soil Microorganisms in the Potato Fields and the Emergence of Variants in Phytophthora infestans 김성은, 문재선, 김정규, 유란희, 최원식, 이은나, 이상한, 김성욱 pp.1027-1031

Expression and Characterization of a Novel Deoxyribose 5-Phosphate Aldolase from Paenibacillus sp. EA001 김용모, 최낙식, 김용욱, 손동호, 장영효, 송재준, 김중수 pp.995-1000

Melanin Synthesis Inhibition and Radical Scavenging Activities of Compounds Isolated from the Aerial Part of Lespedeza cyrtobotrya 이미연, 김진희, 최정남, 김지영, 황겸숙, 이충환 pp.988-994

Characterization of Biochemical Properties of Feline Foamy Virus Integrase 이동현, 김지예, 현우속, 신차균 pp.968-973

Adhesive Properties, Extracellular Protein Production, and Metabolism in the Lactobacillus rhamnosus GG Strain when Grown in the Presence of Mucin Sánchez, Borja, Naïma Saad, Jean-Marie Schmitter, Philippe Bressollier, María C. Urdaci pp.978-984

Effect of gcl, glcB, and aceA Disruption on Glyoxylate Conversion by Pseudomonas putida JM37 Li, Xuan Zhong, Bettina Rosche, Janosch Klebensberger pp.1006-1010

Biotransformation of Bioactive (-)-Mellein by a Marine Isolate of Bacterium Stappia sp Feng, Zhile, Viviane N. Nenkep, Keumja Yun, Dahai Zhang, 최홍대, 강정숙, 손병화 pp.985-987

Characterization of Drug-Resistant Salmonella enterica Serotype Typhimurium by Antibiograms, Plasmids, Integrons, Resistance Genes, and PFGE Benacer, Douadi, Kwai Lin Thong, Savithri Devi Puthucheary, Haruo Watanabe pp.1042-1052

Implications of SPION and NBT Nanoparticles upon In Vitro and In Situ Biodegradation of LDPE Film Kapri, Anil, Reeta Goel, M.G.H. Zaidi pp.1032-1041

Purification and Characterization of Chitinase from a New Species Strain, Pseudomonas sp. TKU008 Wang, San-Lang, Chuan-Lu Wang, Je-Ruei Liu, Bo-Shyun Lin, Tzu-Wen Liang, Pei-Chen Wu pp.1001-1005

New Dioscin-Glycosidase Hydrolyzing Multi-Glycosides of Dioscin from Absidia Strain Fu, Yao Yao, Hong Shan Yu, Si Hui Tang, Xiang Chun Hu, Yuan Hao Wang, Bing Liu, Chen Xu Yu, Feng Xie Jin pp.1011-1017

Expression of Yeast Cyclophilin A(Cpr1) Provides Improved Stress Tolerance in Escherichia coli 김일섭, 신선영, 김영생, 김현영, 이동희, 진익렬, 윤호성, 박경목 pp.974-977

Escherichia coli Arabinose Isomerase and Staphylococcus aureus Tagatose- 6-Phosphate Isomerase : Which is a Better Template for Directed Evolution of Non-Natural Substrate Isomerization? 김혜정, 엄태국, 김성보, 김필 pp.1018-1021

참고문헌 (16건) : 자료제공( 네이버학술정보 )

참고문헌 목록에 대한 테이블로 번호, 참고문헌, 국회도서관 소장유무로 구성되어 있습니다.
번호 참고문헌 국회도서관 소장유무
1 Asboth, B. and G. Naray-Szabo. 2000. Mechanism of action of D-xylose isomerase. Curr. Protein Pept. Sci. 1: 237-254. 미소장
2 Molecular and industrial aspects of glucose isomerase. 네이버 미소장
3 Biotechnology Journal 3/2008 네이버 미소장
4 A new spectrophotometric method for the detection and determination of keto sugars and trioses. 네이버 미소장
5 Kim, P. 2004. Current studies on biological tagatose production using L-arabinose isomerase: A review and future perspective. Appl. Microbiol. Biotechnol. 65: 243-249. 미소장
6 Improvement of tagatose conversion rate by genetic evolution of thermostable galactose isomerase. 네이버 미소장
7 Cobalt proteins. 네이버 미소장
8 Distinct metal dependence for catalytic and structural functions in the L-arabinose isomerases from the mesophilic Bacillus halodurans and the thermophilic Geobacillus stearothermophilus. 네이버 미소장
9 Crystal Structure of Escherichia coliL-Arabinose Isomerase (ECAI), The Putative Target of Biological Tagatose Production 네이버 미소장
10 Substrate Variety of a Non-metal Dependent Tagatose-6-phosphate Isomerase from Staphylococcusaureus. 소장
11 Oh, D. K., H. J. Oh, H. J. Kim, J. Cheon, and P. Kim. 2006. Modification of optimal pH in L-arabinose isomerase from Geobacillus stearothermophilus for D-galactose isomerization. J. Mol. Cat. B Enz. 43: 108-112. 미소장
12 Probing the essential catalytic residues and substrate affinity in the thermoactive Bacillus stearothermophilus US100 L-arabinose isomerase by site-directed mutagenesis. 네이버 미소장
13 Bioconversion of D-galactose into D-tagatose by expression of L-arabinose isomerase. 네이버 미소장
14 Phosphoglucose Isomerase: A Ketol Isomerase with Aldol C2-Epimerase Activity 네이버 미소장
15 Molecular determinants of xylose isomerase thermal stability and activity: analysis of thermozymes by site-directed mutagenesis. 네이버 미소장
16 Increasing the thermostability of D-xylose isomerase by introduction of a proline into the turn of a random coil. 네이버 미소장

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