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An extracellular xylanase was purified to homogeneity by

sequential chromatography of Fomitopsis pinicola culture

supernatants on a DEAE-Sepharose column, a gel filtration

column, and then on a MonoQ column with fast protein

liquid chromatography. The relative molecular mass of the

F. pinicola xylanase was determined to be 58 kDa by sodium

dodecyl sulfate polyacrylamide gel electrophoresis and by

size-exclusion chromatography, indicating that the enzyme

is a monomer. The hydrolytic activity of the xylanase had

a pH optimum of 4.5 and a temperature optimum of 70oC.

The enzyme showed a t1/2 value of 33 h at 70oC and catalytic

efficiency (kcat=77.4 s-1, kcat/Km=22.7 mg/ml/s) for oatspelt

xylan. Its internal amino acid sequences showed a significant

homology with hydrolases from glycoside hydrolase (GH)

family 10, indicating that the F. pinicola xylanase is a

member of GH family 10.

권호기사

권호기사 목록 테이블로 기사명, 저자명, 페이지, 원문, 기사목차 순으로 되어있습니다.
기사명 저자명 페이지 원문 목차
Microencapsulation of Live Probiotic Bacteria Islam, Mohammad Ariful, 윤철희, 최윤재, 조종수 pp.1367-1377

Quantitative Detection of Residual E. coli Host Cell DNA by Real-Time PCR 이동혁, 배정은, 이정희, 신정섭, 김인섭 pp.1463-1470

Antimicrobial Susceptibility of Coagulase-Negative Staphylococci Isolated from Bovine Mastitis Between 2003 and 2008 in Korea 남향미, 임숙경, 김재명, 강현미, 문진산, 장검찬, 주위석, 정숙찬, 김종만, 위성환 pp.1446-1449

Acidophilic Tannase from Marine Aspergillus awamori BTMFW032 Beena, P. S., G. Bhat Sarita, M. Chandrasekaran, M. B. Soorej, K. K. Elyas pp.1403-1414

Biodegradation of Di-n-Butyl Phthalate by Rhodococcus sp. JDC-11 and Molecular Detection of 3,4-Phthalate Dioxygenase Gene Jin, De-Cai, Ren-Xing Liang, Qin-Yun Dai, Rui-Yong Zhang, Xue-Ling Wu, Wei-Liang Chao pp.1440-1445

Fungicidal Effect of Prenylated Flavonol, Papyriflavonol A, Isolated from Broussonetia papyrifera(L.) Vent. Against Candida albicans 손호용, 권종숙, 손건호 pp.1397-1402

Chelation of Calcium Ions by Poly(γ-Glutamic Acid) from Bacillus subtilis(Chungkookjang) Tsujimoto, Takashi, Junya Kimura, 박청, 성문희, Yasushi Takeuchi, Hiroshi Uyama pp.1436-1439

Change of Bacillus cereus Flavonoid O-Triglucosyltransferase Into Flavonoid O-Monoglucosyltransferase by Error-Prone Polymerase Chain Reaction 정나리, 조은지, 김봉규, 안병찬, 박준철, 정유훈, 안중훈 pp.1393-1396

Rheinheimera aquatica sp. nov., Antimicrobial Activity-Producing Bacterium Isolated from Freshwater Culture Pond Chen, Wen-Ming, Chiu-Chung Young, Shih-Yi Sheu, Chang-Yi Lin pp.1386-1392

Genetic Characterization of Atypical Shigella flexneri Isolated in Korea 홍사현, 최연화, 추윤애, 최영운, 최선영, 김동욱, 이복권, 박미선 pp.1457-1462

Isolation of a Novel Freshwater Agarolytic Cellvibrio sp. KY-YJ-3 and Characterization of Its Extracellular β-Agarase 이영준, 한초롱, 김원찬, 준도윤, 이인구, 김영호 pp.1378-1385

The Orientation-Dependent Expression of Angiostatin-Endostatin Hybrid Proteins and Their Characterization for the Synergistic Effects of Antiangiogenesis 백순열, 최신건, 김용석 pp.1430-1435

Comparative Analysis of the Multiple Test Methods for the Detection of Pandemic Influenza A/H1N1 2009 Virus 최영진, 전민혁, 김창진, 황보영, 박귀성, 백경아, 박경배, 남해선, 김휘준, 박준수 pp.1450-1456

Synthesis of an Amphiphilic Poly(γ-Glutamic Acid)-Cholesterol Conjugate and Its Application as an Artificial Chaperone Lee, Eun-Hye, Takashi Tsujimoto, 성문희, Yoshiki Kamigaito, Hiroshi Uyama pp.1424-1429

Purification and Characterization of a Thermostable Xylanase from Fomitopsis pinicola 신검, Marimuthu Jeya, 이정걸, 김영숙 pp.1415-1423

참고문헌 (35건) : 자료제공( 네이버학술정보 )

참고문헌 목록에 대한 테이블로 번호, 참고문헌, 국회도서관 소장유무로 구성되어 있습니다.
번호 참고문헌 국회도서관 소장유무
1 Baldrian, P. and V. Valaskova. 2008. Degradation of cellulose by basidiomycetous fungi. FEMS Microbiol. Rev. 32: 501-521. 미소장
2 Baraznenok, V. A., E. G. Becker, N. V. Ankudimova, and N. N. Okunev. 1999. Characterization of neutral xylanases from Chaetomium cellulolyticum and their biobleaching effect on eucalyptus pulp. Enzyme Microb. Technol. 25: 651-659. 미소장
3 Enzymes in farm animal nutrition 네이버 미소장
4 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding 네이버 미소장
5 Thermozymes and their applications: a review of recent literature and patents. 네이버 미소장
6 Application of xylanases in the pulp and paper industry 네이버 미소장
7 Biotechnology in Turkey: an overview. 네이버 미소장
8 Collins, T., C. Gerday, and G. Feller. 2005. Xylanase families and extremophilic xylanases. FEMS Microbiol. Rev. 29: 3-23. 미소장
9 Biotechnology and the Road of Political Change 네이버 미소장
10 Aspergillus enzymes involved in degradation of plant cell wall polysaccharides. 네이버 미소장
11 Biotechnology and bioeconomy in China. 네이버 미소장
12 Purfication and Properties of Three Xylanases from Aspergillus aculeatus 네이버 미소장
13 Crystal structures of decorated xylooligosaccharides bound to a family 10 xylanase from Streptomyces olivaceoviridis E-86. 네이버 미소장
14 Expression of endo-1, 4-beta-xylanase from Trichoderma reesei in Pichia pastoris and functional characterization of the produced enzyme 네이버 미소장
15 Henrissat, B. and A. Bairoch. 1996. Updating the sequencebased classification of glycosyl hydrolases. J. Biochem. 316: 695-696. 미소장
16 Ito, S., A. Kuno, R. Suzuki, S. Kaneko, Y. Kawabana, I. Kusakabe, and T. Hasegawa. 2004. Rational affinity purification of native family 10 xylanase. J. Biotechnol. 110: 137-142. 미소장
17 Chiral Perturbation Theory for Vector Mesons 네이버 미소장
18 Purification and characterization of a thermostable intracellular β-xylosidase from the thermophilic fungus Sporotrichum thermophile 네이버 미소장
19 Biodegradative mechanism of the brown rot basidiomycete Gloeophyllum trabeum: evidence for an extracellular hydroquinone-driven fenton reaction 네이버 미소장
20 Cleavage of structural proteins during the assembly of the head of bacteriophage T4. 네이버 미소장
21 Biological pretreatment of softwood Pinus densiflora by three white rot fungi. 소장
22 Purification and characterization of a thermostable xylanase from the brown-rot fungus Laetiporus sulphureus(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY) 네이버 미소장
23 Lucena-Neto, A. S. and E. X. F. Filho. 2004. Purification and characterization of a new xylanase from Humicola grisea var. thermoidea. Brazilian J. Microbiol. 35: 86-90. 미소장
24 Thermophilic fungi: their physiology and enzymes. 네이버 미소장
25 Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar 네이버 미소장
26 Morris, D. D., M. D. Gibbs, C. W. J. Chin, M. H. Koh, K. K. Y. Wong, R. W. Allison, P. J. Nelson, and P. L. Bergquist. 1998. Cloning of the xynB gene from Dictyoglomus thermophilum Rt46B.1 and action of the gene product on kraft pulp. Appl. Environ. Microbiol. 64: 1759-1765. 미소장
27 Purification and characterization of extracellular xylanase from Streptomyces cyaneus SN32. 네이버 미소장
28 The mechanisms by which family 10 glycoside hydrolases bind decorated substrates. 네이버 미소장
29 Polizeli, M. L., A. C.Rizzatti, R. Monti, H. F. Terenzi, J. A. Jorge, and D. S. Amorim. 2005. Xylanases from fungi: Properties and industrial applications. Minireview. Appl. Microbiol. Biotechnol. 67: 577-591. 미소장
30 Purification and Properties of Family-10 Endo-1,4-β-Xylanase from Penicillium citrinum and Structural Organization of Encoding Gene(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY) 네이버 미소장
31 Biotechnology: What Now? 네이버 미소장
32 Yan, Q. J., L. Wang, Z. Q. Jiang, S. Q. Yang, H. F. Zhu, and L. T. Li. 2009. A xylose-tolerant β-xylosidase from Paecilomyces thermophila: Characterization and its co-action with the endogenous xylanase. Bioresource Technol. 99: 5402-5410. 미소장
33 Yu, E. K. C., L. U. L. Tan, M. K.-H. Chan, L. Deschatelets, and J. N. Saddler. 1987. Production of thermostable xylanase by a thermophilic fungus, Thermoascus aurantiacus. Enzyme Microb. Technol. 9: 16-24. 미소장
34 Cloning of a xylanase gene from Aspergillus usamii and its expression in Escherichia coli. 네이버 미소장
35 Mapping Glycoside Hydrolase Substrate Subsites by Isothermal Titration Calorimetry 네이버 미소장

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