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Three-dimensional (3D) culture system is useful technique for study of in vivo environment and it was used various experiments. This study was investigated to establish of embryo co-culture system and changes of PAs activity in 3D cultured endometrial cells of pigs. In results, growth of stromal cells into gel matrix were detected only with endometrial and myometrial cells. The most rapid growth of stromal cells were confirmed in 2.5×105 cells/ml and gel matrix containing 15% FBS. Expression of urokinase-PA (uPA) after treatment of hCG (0.5, 1.0, 1.5 and 2.0 IU/ml) were higher than without hCG, but, there are not significant difference among the treatment. On the other hand, expression of uPA after treatment of IL-1β (0.1, 1, 10 and 100 ng/ml) were higher than without IL-1β, but, there are not significant difference. Expression of uPA after treatment of estrogen (0.2, 2, 20 and 200 ng/ml) were not difference, but PA activity was significantly decreased (p<0.05). Blastocyst was producing in PZM-3 medium containing FBS and endometrial cells were grown in PZM-3 medium. When embryos development with cultured endometrial cells, cleavage rates were not significant difference and blastocyst were not produced in co-culture with stromal cells and 3D culture system. 3D culture system had similar activity to in vivo tissue and these features are very useful for study of in vivo physiology. Nevertheless 3D culture system was not proper in embryo co-culture system. Therefore, we suggest that 3D culture system with embryo co-culture need continuous research.

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기사명 저자명 페이지 원문 목차
Comparison of Two Different Serum-free Media for In Vitro Culture of Bovine Embryos Se-woong Kim, Yeon-Gil Jung, Jong-im Park, Sangho Roh pp.229-234

포유류 초기 배아의 효율적인 동결 보존 방법에 관한 연구 김현, 조영무, 고응규, 김성우, 성환후, 야마노우치 케이타로 pp.241-248

OPU 채란계절이 한우의 난자 품질 및 발달 능력에 미치는 영향 김성수, 최병현, 조현태, 진종인, 하아나, 민찬식, 조규완, 공일근 pp.265-271

돼지 자궁 세포의 3차원 배양이 Plasminogen 활성과 수정란 발육에 미치는 영향 이상희, 황보용, 차혜진, 김수지, 김민경, 정희태, 양부근, 박춘근 pp.207-219

OPU 유래 한우 수정란 생산 및 이식 진종인, 최병현, 김성수, 조현태, 선두원, 임현태, 이정규, 민찬식, 공일근 pp.273-281

제주마 집단의 혈연 정보량과 정보 오류가 유전 모수 추정치에 미치는 영향 김남영, 이성수, 양영훈 pp.289-296

Culture Conditions for In Vitro Maturation of Abattoir Derived Oocytes of Native Zebu Cows of Bangladesh S.M. Niyaz Morshed, Mohammad Musharraf Uddin Bhuiyan, Mohammad Moshiur Rahman, Joydev Kumer Singha, Nasrin Sultana Juyena pp.201-206
Influence of Oxygen Consumption on Pregnancy Rates of Hanwoo Calves following Embryo Transfer Hyun Kim, Nan-Hee Bok, Sung-Woo Kim, Yoon-Jung Do, Hwan-Hoo Seong, Jae-Hwan Kim, Dong Hun Kim, Min-Kyu Kim, Yeoung-Gyu Ko pp.257-264

Identification of Niche Conditions Supporting Short-term Culture of Spermatogonial Stem Cells Derived from Porcine Neonatal Testis Min Hee Park, Ji Eun Park, Min Seong Kim, Kwon Young Lee, Jung Im Yun, Jung Hoon Choi, Eunsong Lee, Seung Tae Lee pp.221-228

포유류 초기 배아의 동결 시 생존율에 미치는 Ethylene Glycol(EG)의 영향 김현, 조영무, 고응규, 김성우, 성환후, 야마노우치 케이타로 pp.235-240

과산화수소와 동결에 의해 유도된 생쥐와 소 수정란의 사멸에 있어서 칼륨 통로의 역할 최창용, 김창운, 강다원, 한재희 pp.249-255
칡소의 모색과 Melanocortin 1 Receptor(MC1R) mRNA : 3'-비번역 부위의 변이 및 발현 이해이, 박재희, 김종국 pp.297-303

과배란 처리에 있어 성감별 정액을 이용한 한우 체내 수정란의 생산 효율 전향아, 염규태, 박해금, 김성우, 김현, 김영신, 성환후, 조영무, 조재현, 고응규 pp.283-287

Comparison of Reproductive Parameters of Sow at the Rural Areas of Rangamati District of Bangladesh Sumen Chakma, Pantu Kumar Roy, Ashit Kumar Paul, M. Golam Shahi Alam, Nasrin Sultana Juyena pp.305-311

성장 단계별 희소 한우(칡소)의 혈액학적 분석 김현, 조영무, 고응규, 김성우, 성환후 pp.313-319

참고문헌 (68건) : 자료제공( 네이버학술정보 )

참고문헌 목록에 대한 테이블로 번호, 참고문헌, 국회도서관 소장유무로 구성되어 있습니다.
번호 참고문헌 국회도서관 소장유무
1 Multifunctional roles of urokinase plasminogen activator (uPA) in cancer stemness and chemoresistance of pancreatic cancer. 네이버 미소장
2 Whole-organ tissue engineering: decellularization and recellularization of three-dimensional matrix scaffolds. 네이버 미소장
3 Reproductive Endocrine and Endometrial Effects of Raloxifene Hydrochloride, a Selective Estrogen Receptor Modulator, in Women with Regular Menstrual Cycles 네이버 미소장
4 Balazi A, Sirotkin AV and Chrenek P. 2012. Activators of protein kinase A and oxytocin affect rabbit reproduction. Cent. Eur. J. Biol. 7:973-979. 미소장
5 Biotechnology Journal 2013 Cover Gallery 네이버 미소장
6 Novel pathways for implantation and establishment and maintenance of pregnancy in mammals. 네이버 미소장
7 The novel effect of hCG administration on luteal function maintenance during the estrous cycle/pregnancy and early embryo development in the pig. 네이버 미소장
8 Brännström M, Lind AK and Dahm-Kähler P. 2010. Ovulation:A molecular view. Reproductive Endocrinology and Infertility. Springer, New York, pp. 119-132. 미소장
9 Effects of co-culture with fibroblasts and oviduct cells on in vitro production of porcine embryos 네이버 미소장
10 Human Early Placental Development: Potential Roles of the Endometrial Glands 네이버 미소장
11 Carriero MV, Franco P, Votta G, Longanesi-Cattani I, Vento MT, Masucci MT, Mancini A, Caputi M, Iaccarino I and Stoppelli MP. 2011. Regulation of cell migration and invasion by specific modules of uPA: Mechanistic insights and specific inhibitors. Curr. Drug Targets 12:1761-1771. 미소장
12 Cha HJ, Lee SH, Cheong HT, Yang BK and Park CK. 2013. Analysis of plasminogen activators activity and three dimensional (3D) culture of endometrial cells in pigs. J. Emb. Trans. 28:273-280. 미소장
13 Microarray analysis of human adipose-derived stem cells in three-dimensional collagen culture: osteogenesis inhibits bone morphogenic protein and Wnt signaling pathways, and cyclic tensile strain causes upregulation of proinflammatory cytokine regulators and angiogenic factors. 네이버 미소장
14 Chen J, Dosier CR, Park JH, De S, Guldberg RE, Boyan BD and Schwartz Z. 2013. Mineralization of three dimensional osteoblast cultures is enhanced by the interaction of 1α, 25dihydroxyvitamin D3 and BMP2 via two specific vitamin D receptors. J. Tissue. Eng. Regen. M. 미소장
15 Viral screening of spent culture media and liquid nitrogen samples of oocytes and embryos from hepatitis B, hepatitis C, and human immunodeficiency virus chronically infected women undergoing in vitro fertilization cycles 네이버 미소장
16 Vasculogenesis and angiogenesis in the endometrium during menstrual cycle and implantation 네이버 미소장
17 Mouse embryonic stem cell culture for generation of three-dimensional retinal and cortical tissues. 네이버 미소장
18 Expression of several components of the plasminogen activator and matrix metalloproteinase systems in endometriosis 네이버 미소장
19 Hypoxia Promotes Progesterone Synthesis During Luteinization in Bovine Granulosa Cells 네이버 미소장
20 Impaired placental vasculogenesis compromises the growth of sheep embryos developed in vitro. 네이버 미소장
21 Gadella BM and Evans JP. 2011. Membrane fusions during mammalian fertilization. Cell Fusion in Health and Disease. Springer, Netherlands, pp. 65-80. 미소장
22 Effect of presynchronization with human chorionic gonadotropin or gonadotropin-releasing hormone 7 days before resynchronization of ovulation on fertility in lactating dairy cows 네이버 미소장
23 How Is Plasminogen/Plasmin System Contributing to Regulate Sperm Entry Into the Oocyte? 네이버 미소장
24 Differential Expression and Regulation of Angiopoietin-2 in the Mouse Ovary During Sexual Maturation and Luteal Development. 네이버 미소장
25 Hansen PJ. 2014. Current and future assisted reproductive technologies for mammalian farm animals. Current and Future Reproductive Technologies and World Food Production. Springer, New York, pp. 1-22. 미소장
26 Haraguchi S, Dang-Nguyen T, Kikuchi K, Tanihara F, Bodo S, Somfai T, Akagi S, Hirao Y, Watanabe S and Nagai T. 2014. 157 Leukemia inhibitory factor improves oocyte maturation and developmental competence in pigs. Reproduction, Fertility and Development 26:192-192. 미소장
27 Endometrial glands as a source of nutrients, growth factors and cytokines during the first trimester of human pregnancy: a morphological and immunohistochemical study. 네이버 미소장
28 Embryo-maternal signalling: how the embryo starts talking to its mother to accomplish implantation 네이버 미소장
29 Selection of In Vitro-Matured Porcine Oocytes Based on Localization Patterns of Lipid Droplets to Evaluate Developmental Competence 네이버 미소장
30 Ip MM and Darcy KM. 1996. Three-dimensional mammary primary culture model systems. J. Mammary. Gland. Biol. 1:91-110. 미소장
31 trans‐10, cis‐12 conjugated linoleic acid enhances in vitro maturation of porcine oocytes 네이버 미소장
32 Changes in Plasminogen Activity in Uterus Tissue during the Estrous Cycle in the Pigs 소장
33 Concentration of plasminogen activators and inhibitor in the human endometrium at different phases of the menstrual cycle. 네이버 미소장
34 Oxidized Phospholipids Are Present on Plasminogen, Affect Fibrinolysis, and Increase Following Acute Myocardial Infarction 네이버 미소장
35 Endogenous Tissue Plasminogen Activator Enhances Fibrinolysis and Limits Thrombus Formation in a Clinical Model of Thrombosis 네이버 미소장
36 Urokinase-type plasminogen activator (uPA) induces pulmonary microvascular endothelial permeability through low density lipoprotein receptor-related protein (LRP)-dependent activation of endothelial nitric-oxide synthase. 네이버 미소장
37 Regulation of proteinases during mouse peri-implantation development: urokinase-type plasminogen activator expression and cross talk with matrix metalloproteinase 9. 네이버 미소장
38 Effect of systemic progesterone concentration on the expression of progesterone-responsive genes in the bovine endometrium during the early luteal phase. 네이버 미소장
39 Merrick D, Chen H-C, Larner D and Smith J. 2010. Adult and embryonic skeletal muscle microexplant culture and isolation of skeletal muscle stem cells. J. Vis. Exp. 43:2051. 미소장
40 Production of Middle White Piglets after Transfer of Embryos Produced In Vitro 네이버 미소장
41 Glucose and glycine synergistically enhance the in vitro development of porcine blastocysts in a chemically defined medium. 네이버 미소장
42 Vascular endothelial growth factor expression in the endometrium during the menstrual cycle, implantation window and early pregnancy. 네이버 미소장
43 Effects of β-glucan polysaccharide revealed by the dominant lethal assay and micronucleus assays, and reproductive performance of male mice exposed to cyclophosphamide. 네이버 미소장
44 Low doses of celecoxib stimulate human endometrium growth in a three-dimensional culture model. 네이버 미소장
45 Effect of eCG on early resumption of ovarian activity in postpartum dairy cows 네이버 미소장
46 Plasminogen Activators in Tissue Remodeling and Invasion: mRNA Localization in Mouse Ovaries and Implanting Embryos 네이버 미소장
47 Sart S, Tsai AC, Li Y and Ma T. 2013. Three-dimensional aggregates of mesenchymal stem cells:cellular mechanisms, biological properties, and applications. Tissue Eng. Part B:Rev. 미소장
48 The osteogenic differentiation of adult bone marrow and perinatal umbilical mesenchymal stem cells and matrix remodelling in three-dimensional collagen scaffolds 네이버 미소장
49 Recruitment of Neutrophils Within the Pregnant Corpus Luteum in Response to Interferon Tau at the Maternal Recognition Period in the Cow. 네이버 미소장
50 Promising system for selecting healthy in vitro-fertilized embryos in cattle. 네이버 미소장
51 Molecular mechanisms of luteinization 소장
52 Effects of amino acid supplements and replacement of polyvinyl alcohol with bovine serum albumin in porcine zygote medium. 네이버 미소장
53 Excessive fibrin deposition in nasal polyps caused by fibrinolytic impairment through reduction of tissue plasminogen activator expression. 네이버 미소장
54 Evaluation of Zona Pellucida Function for Sperm Penetration During In Vitro Fertilization in Pigs 네이버 미소장
55 Cell-type specificity of interleukins 1α and 1β on prostaglandin and plasminogen activator production in bovine endometrial cells 네이버 미소장
56 Selenium and Vitamin E Improve the In Vitro Maturation, Fertilization and Culture to Blastocyst of Porcine Oocytes 네이버 미소장
57 Tissue plasminogen activator and plasminogen activator inhibitor 1 contribute to sonic hedgehog-induced in vitro cerebral angiogenesis. 네이버 미소장
58 In vivo fertilising ability of frozen-thawed boar sperm treated with cholesterol-loaded cyclodextrins prior to cryopreservation 네이버 미소장
59 A three-dimensional co-culture model of the aortic valve using magnetic levitation 네이버 미소장
60 Impaired fertilizing ability of superoxide dismutase 1-deficient mouse sperm during in vitro fertilization. 네이버 미소장
61 Predictive factors in in vitro fertilization (IVF): a systematic review and meta-analysis 네이버 미소장
62 Vidi PA, Bissell MJ and Lelièvre SA. 2013. Three-dimensional culture of human breast epithelial cells:the how and the why. Epithelial Cell Culture Protocols. Springer, Humana Press, pp. 193-219. 미소장
63 Three-dimensional modeling of ovarian cancer 네이버 미소장
64 Effect of the administration of GnRH or hCG on time of ovulation and the onset of estrus-to-ovulation interval in sows in Thailand. 네이버 미소장
65 Tissue plasminogen activator enhances mobilization of endothelial progenitor cells and angiogenesis in murine limb ischemia. 네이버 미소장
66 Development and Application of a Chemically Defined Medium for the In Vitro Production of Porcine Embryos 네이버 미소장
67 Activation of PI3K/mTOR signaling pathway contributes to induction of vascular endothelial growth factor by hCG in bovine developing luteal cells 네이버 미소장
68 Mechanisms for the Establishment of Pregnancy in the Pig 네이버 미소장

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